Variant cathelicidin-BF15 of cathelicidin-BF and use thereof
A kind of antimicrobial peptide and variant technology of golden krait, which is applied in the field of biomedicine, can solve the problems of unreported antimicrobial peptide research, and achieve the effect of artificial synthesis of broad-spectrum antibacterial, strong killing effect and strong bactericidal effect
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[0016] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.
[0017] Preparation of cathelicidin-BF15:
[0018] I. Preparation method of cathelicidin-BF15: According to the amino acid sequence of cathelicidin-BF, an antimicrobial peptide of B. kraitus obtained through separation and purification, a mutant cathelicidin-BF15 of cathelicidin-BF was designed by proteomics method. The full sequence was synthesized with an automatic peptide synthesizer (433A, Applied Biosystems), and purified by desalting and HPLC reverse-phase column chromatography.
[0019] II. Molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF).
[0020] III. The purity of the purified cathelicidin-BF15 is identified by high-performance liquid chromatography (HPLC), the molecular weight is determined by matrix-assisted laser desorption ioniza...
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