30 results about "Hormaomycin" patented technology

An abscisic acid (1) is a plant hormone widely existing in a plant and has important physiological function, however, the application range is limited by the high-speed photo-isomerization characteristics. A bioisosteres cis 2, 3-cyclopropanated abscisic acid analogue of the abscisic acid is compounded through 2, 3-cyclopropanation, and the photostability is four times as that of the abscisic acid.

The invention provides a method for screening a I type 17 beta-hydroxysteroid dehydrogenase inhibitor through an immobilized enzyme, and belongs to the technical field of enzymology and enzyme engineering. I type 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD1) has the important function in treating hormone-dependent diseases. At present, a substrate radiolabelling method is mainly adopted in studying the activity of 17 beta-HSD1, due to the fact that the free enzyme of 17 beta-HSD1 is likely to be inactivated, 17 beta-HSD1 is hard to prepare, a fresh placenta is needed for preparing an enzyme source in a new medicine screening experiment of every time, raw materials are hard to obtain, the price is high, and difficulty is brought to the screening work of new medicine. According to the method, an amino-modified silicon ball is adopted as a carrier, a glutaraldehyde crosslinking method is utilized, 17 beta-HSD1 extracted from the placenta is fixed, an external immobilization 17 beta-HSD1 enzyme model is built, androstenedione is adopted as a substrate, a high performance liquid chromatography method is used for detecting products, and the potential 17 beta-HSD1 inhibitor is screened. According to the method, instability of the free enzyme is overcome, operation is easy, the manufacturing cost is low, and repeated using is achieved.

The invention relates to a naphthlamide derivative used as a protein kinase inhibitor and a histone deacetylase inhibitor. The structure of the naphthlamide derivative is as shown as a formula I, wherein a position 7 is provided with substituent groups R which include F, Cl, Br, I, OCH3 and other substituent groups as shown in the accompanying drawings, and the names of the substituent groups arefluorine, chlorine, bromine, iodine, methoxyl, 4-morpholinyl ethyoxyl, 4-morpholinyl methyl phenyl, acryloyl amino and 2-beuteamide. The naphthlamide derivative used as the protein kinase inhibitor and the histone deacetylase inhibitor can be used for curing cardiovascular diseases, metabolic diseases, allergy, cancers and hormone-related diseases. The invention additionally provides a synthesis method of the compound. The method has the advantages that the raw materials are easy to obtain, the method is simple to operate and the method is suitable for large-scale industrial production.

The invention provides a preparation method of natamycin produced by fermentation regulation of Streptomyces gilvosporeus and relates to the technical field of bioengineering. The method comprises steps as follows: strain activation, primary seed culture and shake flask fermentation, wherein the strain is Streptomyces gilvosporeus, ATCC 13326. The influence of three different plant hormones including IAA, GA and 6-BA, as well as precursor substance sodium propionate added in a fermentation process in a compounding manner on Streptomyces gilvosporeus cell growth and natamycin synthesis are investigated in order to understand the regulating effect of the plant hormones and the precursor substance on Streptomyces gilvosporeus cell growth and natamycin synthesis, and experimental evidence is provided for improving the efficiency of Streptomyces gilvosporeus fermentation based natamycin production by the plant hormones and the precursor substance.

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

Non-peptide GnRH agents capable of inhibiting the effect of gonadotropin-releasing hormone are described. Such compounds and their pharmaceutically acceptable salts, prodrugs, and active metabolites are suitable for treating mammalian reproductive disorders and steroid hormone-dependent tumors as well as for regulating fertility, where suppression of gonadotropin release is indicated. Methods for synthesizing the compounds and intermediates useful in their preparation are also described.

Novel Saccharomyces Cerevisiae mutant strains are provided for producing human parathyroid hormone. The novel strains acre genetically disrupted in at least one of the genes encoding the yapsin family of proteases Yapsin 1, Yapsin 2 and Yapsin 3 and harbor a human parathyroid hormone gene in their genomes. Culturing the novel strains results in the secretion of intact hPTH into culture media at high yield.