The invention provides a mite-killing water emulsion, which is prepared by using daucosterol as main raw material and adding a solvent, a solubilizer, an emulsifier and other auxiliary materials to the daucosterol. The mite-killing water emulsion is an environment-friendly acceptable pesticide, which is safe to animals and human beings, and has a profound significance for the long-term development of the ecological environment, the health of the human beings and the comprehensive treatment of the diseases. Compared with the traditional pesticide, the mite-killing water emulsion has the following characteristics and advantages: 1, the daucosterol is abundant in nature, the content is higher especially in plants, the toxicity of the daucosterol is low, and the daucosterol is easily degradable and is safe for the ecological environment; 2, the extracting method of the daucosterol is simpler, and the daucosterol can be extracted by techniques of a liquid phase chromatographic separation method and the like; 3, the daucosterol also can be synthesized artificially, has low cost, is easily used for industrial large-scale production; 4, due to physical and chemical properties, the daucosterol can be dissolved in a plurality of solvents, is easily blended with other mite-killing components for preparing pesticide, and can play a role in improving mite-killing effect; and 5, with soybean oil as solvent, the daucosterol plant miticide water emulsion provided by the invention is non-toxic and environment friendly, can be used for increasing the solubility of the daucosterol in the water and also plays a role in promoting the effect that the pesticide permeates into the plant skin.