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Preparation method of ginkgo leaf lipoid components having antibacterial activities

A technology of antibacterial activity and ginkgo biloba leaves is applied in the field of preparation of lipid components of ginkgo biloba leaves, and can solve the problems of few reports on the bacteriostatic activity of lipid components of fat-soluble components and the like.

Active Publication Date: 2013-05-08
邳州恒翔生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The reported antibacterial active components of Ginkgo biloba are mainly flavonoids, phenolic acids and other components, that is, mainly water-soluble and alcohol-soluble chemical parts, and there are few reports on the antibacterial activity of fat-soluble components, especially lipid components.

Method used

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  • Preparation method of ginkgo leaf lipoid components having antibacterial activities
  • Preparation method of ginkgo leaf lipoid components having antibacterial activities
  • Preparation method of ginkgo leaf lipoid components having antibacterial activities

Examples

Experimental program
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Effect test

Embodiment 1

[0010] The extraction separation method of embodiment 1 ginkgo leaf lipid component

[0011] The preparation method of the ginkgo biloba lipid component with antibacterial activity consists of the following steps:

[0012] The first step, preparation of ginkgo leaf lipid ointment: take 10 kg of dried and crushed ginkgo leaf powder (less than 0.5 mm in diameter), add 30 L of petroleum ether (60-90 ° C), heat and reflux at 60 ° C for 4 hours, and repeat the extraction 3 times Combine the extracts and reclaim the solvent to obtain 500g of ginkgo biloba fat-soluble component ointment; add 6L of 5% NaOH methanol solution, stir at 65°C under reflux, the stirring speed is 60r / min, and the time is 2h to obtain saponified solution; add sherwood oil ( 60~90 ℃) 30L extraction 3 times, combine extraction solution, recover solvent, thereby obtain ginkgo biloba lipid total unsaponifiable matter 350g.

[0013] The second step, the molecular distillation and separation method of ginkgo leaf ...

Embodiment 2

[0015] The antibacterial activity investigation method of embodiment 2 Ginkgo biloba lipid components

[0016] 1. Experimental strains and raw materials

[0017] 1.1 Bacteria to be tested

[0018] Salmonella ATCC14028, Staphylococcus aureus ATCC25923 and Aspergillus niger ATCC16404

[0019] 1.2 Samples to be tested

[0020] Samples such as compound 1~8 and polyprenol obtained in embodiment 1

[0021] 2 Experimental methods

[0022] 2.1 Determination of antibacterial activity of samples by filter paper method

[0023] (1) Preparation of culture medium

[0024] Heat to dissolve, after cooling, adjust the pH to 7.0-7.2, this is a liquid medium, add 2.0g agar powder to it, this is a solid medium, sterilize at 121°C for 20min.

[0025] (2) Liquid medium inoculation and activation of bacteria

[0026] Turn on the ultraviolet sterilizing lamp of the purification workbench. After half an hour of sterilization, turn on the operation switch of the purification station, take a rin...

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Abstract

The invention discloses a preparation method of ginkgo leaf lipoid components having antibacterial activities. Ginkgo leaf which is a raw material is extracted with a non-polar solvent, and undergoes molecular distillation separation, column chromatography, re-crystallization and purification to obtain eight compounds (sequentially comprising isophytol, nerolidol, linalool, beta-sitosterol acetate, beta-sitosterol, stigmasterol, ergosterol and daucosterol), polypentenol, ginkgo leaf lipoid total unsaponifiable matters, ginkgo leaf lipoid crystals, a ginkgo leaf lipoid light fraction and a ginkgo leaf lipoid heavy friction. The eight compounds, polypentenol, the ginkgo leaf lipoid total unsaponifiable matters, the ginkgo leaf lipoid crystals, the ginkgo leaf lipoid light fraction and the ginkgo leaf lipoid heavy friction have inhibition effects on salmonellas, Staphylococcus aureus and Aspergillus niger.

Description

technical field [0001] The invention relates to a preparation method of ginkgo biloba lipid components with antibacterial activity, belonging to the field of biomedicine. technical background [0002] Ginkgo biloba is the leaf of Ginkgo biloba L. in the Ginkgo family. In recent years, scholars at home and abroad have conducted extensive research on the chemical components of flavonoids, terpene lactones, polysaccharides, polyprenol, volatile oils and sterols in Ginkgo leaves. Research. Ginkgo biloba lipid components mainly include hydrocarbons, polypentenols, terpene alcohols, sterols and other compounds, which are similar in polarity and difficult to separate; Ginkgo biloba lipid components mainly exist in the form of oily substances. There are usually white crystals precipitated, which are mainly sterols according to reports. Ginkgo biloba sterols are mainly reported on the respective chemical structures of their mixtures by gas chromatography-mass spectrometry (GC-MS). ...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61P31/04A61P31/10C07C33/03C07C33/02C07C29/76C07C29/78C07J9/00C07J17/00A61K127/00
CPCY02A50/30
Inventor 王成章陶冉
Owner 邳州恒翔生物制品有限公司
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