38 results about "Cell studies" patented technology

The invention provides a method for preparing hydrogel microparticles based on a paper chip technology. A paper chip consists of patterns which are sprayed and printed on paper by a wax spray printer, and is immersed in a solution containing calcium ions and alginate to form hemispheric alginate hydrogel microparticles with codes; and the formed hemispheric microparticles can be combined under a microscope to form asymmetrical spherical hydrogel microparticles. The asymmetrical microparticles can be applied to mutual effects among cells, the irritation influence of medicines on the cells and the like, and have an excellent application prospect on the study of the cells, the screening of the medicines and the like. The method is simple, easy to operate and low in cost, and the prepared microparticles have the advantages of uniform sizes, controllable sizes and the like.

An open single-cell research chip and its preparation method, the chip includes a packaging structure surrounded by a substrate, a retaining wall and a transparent cover, the substrate has a microhole array structure inside the packaging structure, the A number of microwells in the microwell array structure contain single cells respectively, and droplets of reagents for single cell research are respectively added to the top of each microwell by 3D printing, and the remaining space in the packaging structure is filled with sealing oil, further providing The manufacturing method of the chip. The invention perfectly combines 3D printing technology with single-cell microwell arrays, efficiently and quickly realizes the research on the single-cell level, establishes a single-cell research system with simple structure, wide application range and perfect functions, and realizes high occupation High-rate single-cell capture, multi-step liquid treatment of single cells with diversity, accuracy, and open structure can be applied to a variety of research systems.

The invention discloses a composition for treating esophageal cancer and its application. The technical scheme is that the invention uses a cell model, is guided by activity tracking, and adopts modern extraction and separation technologies such as alcohol extraction, chromatography, and high performance liquid chromatography. Active monomers were extracted from four traditional Chinese medicines with anti-cancer activity: Pangolinus, Zhuyazao, Tubeimu and Chonglou, and the chemical spectrogram method was used to speculate on the molecular composition and determine the molecular structure of each monomer, which was determined by the baseline equal ratio increase and decrease method The dosage ratio of the monomers is combined into a composition for treating esophageal cancer, and then the active monomers and the composition are used to act on various esophageal cancer cells to study their mechanism of action on esophageal cancer. The four active monomers and their compositions provided by the invention are all derived from the extraction of natural medicines, are pure traditional Chinese medicine preparations, have the functions of preventing and treating esophageal cancer, and have great market potential and broad market application prospects.