Purpose of hydrogen gas to preparation of renal fibrosis treatment preparation

A technology for renal fibrosis and fibrosis, which is applied in the field of preparation of preparations for treating renal fibrosis, and can solve the problems of unsatisfactory effect and the like

Inactive Publication Date: 2018-02-02
FUDAN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on renal fibrosis in the prior art focuses on preventing these three stages of renal fibrosis formation, and correcting related factors, such as infection, hypertrophy of residual nephrons, urinary protein, hypertension, hyperglycemia, hypernitrogenous blood disease and metabolic acid

Method used

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  • Purpose of hydrogen gas to preparation of renal fibrosis treatment preparation
  • Purpose of hydrogen gas to preparation of renal fibrosis treatment preparation
  • Purpose of hydrogen gas to preparation of renal fibrosis treatment preparation

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Embodiment 1

[0045] Materials and methods: methanol (Sinopharm Chemical Reagent Co., Ltd.), paraformaldehyde (Sinopharm Chemical Reagent Co., Ltd.), ethanol (Sinopharm Chemical Reagent Co., Ltd.), cell culture medium: RPMI 1640 (Gibco), fetal bovine serum ( Hyclone), PBS phosphate buffered saline, trypsin (Shanghai Boguang Biotechnology Co., Ltd.), TGF-beta1 (R&D), DMSO (Sigma), PageRulerTM Prestmned Protein Ladder (Fermentas) 11.Mousemonoclonal to alpha muscle Actin (Abcam), E-cadhefin rabbit monoclonal antibody(Epitomics), PAl-1rabbit monoclonal antibody(Epitomics), HRP-labeled goat anti-rabbit IgG(H+L), HRP-labeled goat anti-mouse IgG(H+L)(Mingrui Biology), beta -Actin antibody (Sigma), BSA (Sigma), hydrogen peroxide (Sinopharm Chemical Reagent Co., Ltd.), hematoxylin stain (Wuhan Google Biotechnology Co., Ltd.), hydrochloric acid, ammonia (Sinopharm Chemical Reagent Co., Ltd.), neutral gum ( Google Bio), . Histochemical Kit DAB Chromogenic Reagent (DAKO);

[0046] Main instruments and...

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Abstract

The invention belongs to the field of biopharmacology, and relates to a purpose of hydrogen gas to preparation of renal fibrosis treatment medicine, in particular to a purpose of hydrogen gas to preparation of medicine for treating renal fibrosis caused by chronic nephrosis. A built animal model is subjected to biochemical index detection, isolated cell study, western blotting experiments, immunohistochemistry and data analysis after hydrogen gas intervening; the result shows that the hydrogen gas can realize the fibrosis inhibition on the pathological environment induction through an sirt1 path; the harm to the renal function unit in the fibrosis process is reduced through improving the sirt1; and the hydrogen gas can be further used for preparing a medicine preparation for treating renalfibrosis, particularly the renal fibrosis caused by chronic nephrosis.

Description

technical field [0001] The invention belongs to the field of biopharmacology, and relates to the new use of hydrogen in pharmacy, in particular to the use of hydrogen in the preparation of preparations for treating renal fibrosis, especially the use of hydrogen in the preparation and treatment of various chronic kidney diseases in long-term diseases. Use in preparations of tubulointerstitial fibrosis produced during the process. Background technique [0002] Studies have shown that renal fibrosis (including glomerular fibrosis, renal interstitial fibrosis, and renal vascular fibrosis) is a common channel and common pathological change for almost all renal diseases to progress to end-stage renal failure, and it is also a common pathological change in the end-stage chronic kidney disease. One of the important reasons for the complete loss of renal function in patients; while the current intervention solution is only regular dialysis and kidney transplantation; practice shows t...

Claims

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Application Information

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IPC IPC(8): A61K33/00A61P13/12
CPCA61K33/00
Inventor 范敏张雪梅邢兆宇潘万马陶杰蓝珍
Owner FUDAN UNIV
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