82 results about "Cell enrichment" patented technology

Methods and devices for isolating and diagnosing disease with a cell adhesion matrix system, mimicking a metastatic, cardiovascular or placental environment, are disclosed. The cell adhesion matrix facilitates the enrichment of target cells such as metastatic tumor cells, fetal cells and endothelial progenitor cells from a fluid sample such as blood for diagnostic and therapeutic applications in treating patients afflicted with disease, such as cancerous, cardiovascular and fetal diseases, as well as for research applications in molecular analysis of metastatic, and cardiovascular and fetal diseases. Blood test prototypes and methods for the cell enrichment and detection of circulating tumor and endothelial cells using multiplex molecular analysis are described herein. In addition, methods and compositions for determining host immunity to tumor in subjects with risk of cancer progression and methods for isolating an enriched fraction of fetal cells from pregnant females for prenatal diagnosis are also described herein.

Disclosed herein are methods and systems for the concentration of cells from a cell suspension into unprocessed tissue, such as adipose tissue. Also disclosed herein are systems for optimizing hydration of tissue and cell enriched grafts.

The invention discloses a method and an automatic instrument device for enriching and extracting target cells and separating single cells by using a positive magnetic bead method and performing immunity and molecular biology identification and analysis on single cells. By the method and the instrument device, the on / off of a capture magnet and a release magnet is controlled by various methods to complete target cell searching, capturing, cleaning and releasing operation once or for multiple times, so that the target cell detection sensitivity and stability are improved. When the capture magnet searches and captures the target cells, the search line is circular, square, comb-shaped, S-shaped or U-shaped. In addition, the captured substances are filtered, most free micro magnetic beads are removed, the purity of the product is further improved and the product can be used as a good experimental material. By combining a special filter and the adsorption of the capture magnet, more than 95 percent of free micro magnetic beads can be effectively removed. Meanwhile, the types of the single cells are identified and biological characteristics of the single cells are analyzed by an immunofluorescence staining method and a method in molecular biology, and effective biological indexes are provided for clinical diagnosis and treatment of cancers.

A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for lymphoid lineages, but lacking the potential to differentiate into myeloid and erythroid lineages. Methods are provided for the isolation and culture of this common lymphoid progenitor cell (CLP). The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor α); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. The murine cells are also characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are predominantly cycling, blast cells. These cells give rise to B cells, T cells and natural killer cells, as evidenced by their growth and differentiation in vitro and in vivo.

The present invention provides a cell enrichment / purification device having a function of continuously enriching cells, a function of locating the cells in a particular area of a flow path in a continuous array after the cell enrichment, a function of recognizing the shape of the cells and fluorescence emission from the cells at the same time in units of one cell based on an image, and a function of recognizing the cells based on the information on the shape and fluorescence emission to separate / purify the cells.

A substantially enriched mammalian hematopoietic cell population is provided, which is characterized as a progenitor cell committed to the megakaryocyte lineage. Methods are provided for the isolation and culture of these cells. The cell enrichment methods employ reagents that specifically recognize CD9 and CD41, in conjunction with other markers expressed on lineage committed progenitor cells. These cells give rise exclusively to megakaryocytes and platelets, as evidenced by their growth and differentiation in vitro and in vivo.

The present invention relates to a method and a system for automatically recognizing rare cells. The method comprises: injecting a cell enrichment liquid carrying rare cells into a prepared coating-slice device, staining the cells according to a standard immunostaining process, and carrying out multi-fluorescence channel shooting through a fluorescence microscope to obtain a fluorescence image; treating the fluorescence image into a clean image strictly having a background gray value of zero and retaining the true fluorescence value in the cell contour, and carrying out cell contour extraction on the clean image; and carrying out statistics on the average fluorescence intensity value of various extracted contour channels corresponding to the multi-fluorescence channel, and determining the rare cells according to the statistics results. According to the present invention, the obtained fluorescence image is treated to strictly achieve the background gray value of zero while the true fluorescence value in the cell contour is retained, such that the influence of the background noise on the cell contour extraction is minimized; and for the fluorescence image obtained through the multi-fluorescence channel, the cells are confirmed and recognized from more parameters so as to improve the staining recognizing of the rare cells.

Devices for fluid control and biological particle manipulation (e.g., cell enrichment and blood sampling) are disclosed. The devices a based on the ability to control the flow of fluids through the use of microfluidic valves. The valves are characterized, for example, by microstructures disposed on a mobile diaphragm.

A method of generating an isolated population of cells comprising anti-third party cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance-inducing cells and / or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) contacting peripheral blood mononuclear cells (PBMC) with a third party antigen or antigens in the presence of IL-21 so as to allow enrichment of antigen reactive cells; and (b) culturing the cells resulting from step (a) in the presence of IL-21, IL-15 and IL-7 in an antigen free environment so as to allow proliferation of cells comprising the central memory T-lymphocyte (Tcm) phenotype.

The invention discloses joint application of three types of monoclonal antibody-coupled immunomagnetic beads to sorting tumor cells and a method for sorting breast cancer tumor cells. The three types of monoclonal antibody-coupled immunomagnetic beads include anti-HLA-G (human leukocyte antigen-G) monoclonal antibody-coupled immunomagnetic beads, anti-EpCAM (epithelial cell adhesion molecule) monoclonal antibody-coupled immunomagnetic beads and anti-CK8 / 18 (casein kinase 8 / 18) monoclonal antibody-coupled immunomagnetic beads. The joint application and the method have the advantages that the three types of jointly applied antibody-coupled immunomagnetic beads are high in breast cancer tumor cell enrichment ratio and good in sensitivity and repeatability, and the relevant tumor cells can be detected; the three types of immunomagnetic beads (the anti-EpCAM immunomagnetic beads, the anti-HLA-G immunomagnetic beads and the anti-CK8 / 18 immunomagnetic beads) are jointly applied to enriching the tumor cells, so that the circulating tumor cell coverage rate can be increased to the greatest extent, the enrichment ratio can reach 80%, and a technology is international pioneer; the tumor cell enrichment specificity and sensitivity can be particularly guaranteed owing to usage of the anti-HLA-G monoclonal antibody-coupled immunomagnetic beads.

The invention discloses a method for high efficiency enrichment culture of microalgae. According to the invention, an airlift bioreactor and an illuminated airlift bioreactor are in series connection; the airlift bioreactor contains microalgae and a microalgae culture medium, air is introduced to realize airlift and aerobic culture, and a microalgae cell enrichment apparatus is employed in a liquid phase loop; the illuminated airlift bioreactor contains microalgae enriched from the airlift bioreactor and a microalgae culture medium, gas containing CO2 is introduced to realize airlift, supply of CO2 and desorption of O2, and the introduced gas containing CO2 is industrial CO2-containing tail gas which passes through a conveying pretreatment apparatus and then enters into the illuminated airlift bioreactor. Compared with the prior art, the method provided by the invention has the following advantages: high efficiency enrichment of microalgae cells and accumulation of high-content lipid of the microalgae cells are realized, a utilization rate of CO2 is increased, O2 desorption capability is enhanced, culture apparatuses are reduced, etc.

A method for automated cell enrichment preparation where biological cells are injected into a first subchamber and flowed through the first subchamber, through a large pore filter and into a second subchamber so that the large pore filter retains particles large particles in the first subchamber, while the biological cells pass through the first filter into the second subchamber and are retained by a small pore filter. After clearing, the biological cells are stained. A solvent exchange leaves the biological cells in solvent and they are released into a concentration module where a portion of the solvent is removed to form an enriched concentrated cell suspension.

A method of generating an isolated population of cells comprising anti-third party cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance-inducing cells and / or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) contacting peripheral blood mononuclear cells (PBMC) with a third party antigen or antigens in the presence of IL-21 so as to allow enrichment of antigen reactive cells; and (b) culturing the cells resulting from step (a) in the presence of IL-21, IL-15 and IL-7 in an antigen free environment so as to allow proliferation of cells comprising the central memory T-lymphocyte (Tcm) phenotype.

The invention discloses a high-throughput detection method for metal ions and metal nanoparticles based on single cell enrichment and single cell mass spectrometry. The invention provides a method fordetecting the metal ions and metal nanoparticles. The method comprises the following steps: absorbing and enriching the metal ions and the metal nanoparticles by utilizing cells or unicellular organisms; marking the treated cells or single-cell organisms by using a metal isotope A, wherein the metal isotope A is other metal isotopes except a to-be-detected metal element and can be used for marking survival and dead cells or single-cell organisms at the same time; and then carrying out single-cell mass spectrometry flow detection to obtain an intracellular signal intensity value of the to-be-detected metal element, and further calculating to obtain the content of the to-be-detected metal element in a to-be-detected sample. The sample pretreatment is simple, and no harmful reagent is introduced; the demand on samples is very small; the flux is high; the sensitivity is high; cell-related metal signals may be differentiated.

The invention belongs to the field of biological sample analysis and detection, and particularly relates to an integrated system for cell enrichment, separation, dyeing and flaking. The integrated system for cell enrichment, separation, dyeing and faking comprises an automatic control module, and a mechanical control device, a cell separation device and a specimen slide preparation device which can be controlled by the automatic control module in a linkage manner. According to the system disclosed by the invention, CTCs tumor cells are extracted by using the cell separation device in a filtering manner and then transferred to the specimen preparation device for dyeing treatment, and the whole operation process has relatively high automation degree and relatively high flux. All modules ofthe system are reasonable in layout and compact in structure; various functions of sample transferring, sample fixing, sample filtering, cell staining and sample sealing are integrated into one systemfor operation, the whole system is extremely high in integration degree, has the advantages of automation and high flux, can meet the requirement of individualized medical treatment, and is applied to various medical institution occasions, timely diagnosis and the like.

A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte / erythroid progenitor cells (MEP) and granulocyte / monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid / megakaryocytic or myelomonocytic lineages. The cell enrichment methods employ reagents that specifically recognize Thy-1; and IL-7Rα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

The invention provides a cell enrichment and separation method which comprises the following steps: 1) combining a first kind of magnetic beads with a biological sample, wherein the biological sample comprises a first kind of cells and a second kind of cells, the first kind of magnetic beads are combined with a first kind of antibody, and the first kind of cells are combined with the first kind of magnetic beads; 2) putting a capturing magnetic head in the biological sample combined with the first kind of magnetic beads, and adsorbing the first kind of cells on a capturing magnet device; 3) releasing the first kind of cells from the capturing magnetic head device, and eliminating the first kind of cells in the biological sample; 4) adding a second kind of magnetic beads into the biological sample without the first kind of cells, and combining the second kind of cells with the second kind of magnetic beads; 5) putting the capturing magnetic head in the biological sample combined with the second kind of magnetic beads, and adsorbing the second kind of cells on the capturing magnet device; 6) releasing the second kind of cells from the capturing magnetic head device. The method provided by the invention has high detection sensitivity and purity, and can be used for analyzing multiple kinds of gene markers of single cell or a small amount of cells.

In accordance with an embodiment, a microfluidic chip including a first cells enrichment system and a second cells enrichment system is provided. Channel layouts of the first and the second cells enrichment systems are symmetric with respect to a reflection plane vertical to the microfluidic chip. Each of the first the second cells enrichment systems includes a first fluid channel, a second fluid channel, a sample channel, an inlet channel, and a filtration chamber. The sample channel of the first cells enrichment system has a first inner wall and a first outer wall. The sample channel of the second cells enrichment system has a second inner wall and a second outer wall. The first outer wall and the second outer wall are both far from the reflection plane. A distance between the first outer wall and the second outer wall is in the range from about 10 μm to about 1 cm.

The present disclosure relates to an integrated chip, which includes a cell enrichment region, a cell separation region and a cell capture region, wherein one end of the cell enrichment region is provided with an inlet, and the other end of the cell enrichment region is provided with a waste liquid outlet and an enriched liquid outlet; one end of the cell separation region is provided with a buffer solution inlet and an enriched liquid inlet , and the other end of the cell separation region is provided with an outlet; one end of the cell capture region is provided with an inlet, and the other end of the cell capture region is provided with a separated liquid outlet. Compared with the traditional technology, the chip can separate a target cell from a to-be-treated cell solution with a high efficiency, and capture the target cell in situ in a chip.

The invention discloses a cell culture container capable of improving the enrichment efficiency of lung cancer stem cells. The cell culture container comprises a culture container main body, and an enveloping layer is arranged on the inner wall of the culture container main body and is made of gama-polyglutamic acid. The culture container with the gama-polyglutamic acid containing enveloping layer can obviously improve the formation efficiency of suspended grown cell spheres, ideal suspended growing cell spheres can be formed within 24 hours, the lung cancer stem cells can be highly enriched in the suspended growing cell spheres, and the cell culture container has higher efficiency and more convenience compared with the existing serum culture medium-free cell growth factor method.

The invention discloses an application of a difference agency technique to C. T base substitution cell enrichment. A difference agency technique carrier disclosed by the invention comprises the following reagents: sgRNA, a C. T base substitution system and a functional incapacitation screening agent resistant gene, wherein the sgRNA consists of esgRNA targeting a target point sequence of an objective gene and sgRNA targeting a target point sequence of the functional incapacitation screening agent resistant gene; the C.T base substitution system can perform C. T base substitution on the targetpoint sequence of the functional incapacitation screening agent resistant gene under guidance of the sgRNA for targeting the target point sequence of the functional incapacitation screening agent resistant gene so that the functions of the functional incapacitation screening agent resistant gene are recovered. According to the application disclosed by the invention, C.T base substitution cell enrichment on cell level can be realized, and the C.T base substitution efficiency is greatly improved.

In accordance with an embodiment, a microfluidic chip including a first cells enrichment system and a second cells enrichment system is provided. Channel layouts of the first and the second cells enrichment systems are symmetric with respect to a reflection plane vertical to the microfluidic chip. Each of the first the second cells enrichment systems includes a first fluid channel, a second fluidchannel, a sample channel, an inlet channel, and a filtration chamber. The sample channel of the first cells enrichment system has a first inner wall and a first outer wall. The sample channel of thesecond cells enrichment system has a second inner wall and a second outer wall. The first outer wall and the second outer wall are both far from the reflection plane. A distance between the first outer wall and the second outer wall is in the range from about 10[mu]m to about 1cm.

The invention relates to the technical field of cell culture, in particular to a preparation method of high-activity tumor infiltrating lymphocytes which comprises the following steps: step 1, performing density-gradient centrifugation on tumor infiltrating lymphocyte digestive solution treated by collagenase, DNA enzyme and hyaluronidase; seeding the primary tumor infiltrating lymphocytes into a complete RPMI culture medium for proliferation; and seeding the tumor infiltrating lymphocytes at the first passage into a spinner bottle for continuous culture to obtain the high-activity tumor infiltrating lymphocytes. The invention solves the problems of complicated preparation methods and low yield of high-activity tumor infiltrating lymphocytes in the prior art. According to the preparation method, in the preparation process of the tumor infiltrating lymphocyte digestive solution, a comprehensive enzyme digestion method is adopted to digest tissue or cell enrichment, and comprehensive enzyme digestion can ensure the optimal acquisition rate of single cells. The preparation method can be used for preparing clinical tumor infiltrating lymphocytes, and enough lymphocytes can be obtained.

A substantially enriched mammalian hematopoietic cell population is provided, which is characterized as a progenitor cell committed to the megakaryocyte lineage. Methods are provided for the isolation and culture of these cells. The cell enrichment methods employ reagents that specifically recognize CD9 and CD41, in conjunction with other markers expressed on lineage committed progenitor cells. These cells give rise exclusively to megakaryocytes and platelets, as evidenced by their growth and differentiation in vitro and in vivo.

The invention provides a MyD88 gene L265P mutation detection kit based on ARMS-PCR. The MyD88 gene L265P mutation detection kit comprises (1) a peripheral blood mononuclear cell enrichment reagent, (2) protease K, (3) a DNA extraction column, (4) a DNA extraction sleeve, (5) cleaning liquid A, (6) cleaning liquid B, (7) a DNA eluent, (8) an ARMS-PCR specific primer Fw, (9) an ARMS-PCR specific primer Fm, (10) an ARMS-PCR specific primer R0, and (11) an ARMS-PCR reaction system reagent. A mismatch is artificially introduced to the 3'end of the specific primers to improve the specificity of thespecific primers. The concentrations of a DNA template and the primers in the reaction are controlled at a low level to ensure the specificity of the reaction.

A microfluidic device comprises at least one inlet for receiving a sample comprising target cells and non-target cells; a first spiral channel portion having an upstream end in a central region and a downstream end in a peripheral region, the upstream end being coupled to the inlet, the first spiral channel portion being configured such that the target cells and the non-target cells occupy different streams at the downstream end; a first waste outlet arranged to couple with streams of non-target cells at the downstream end of the first spiral channel portion; a link channel portion arranged to couple with streams of target cells at the downstream end of the first spiral channel portion; a second spiral channel portion having an upstream end in a peripheral region and a downstream end in a central region, the upstream end of the second channel portion being coupled to the link channel portion, the second spiral channel portion being configured such that the target cells and the non-target cells occupy different streams at the downstream end; a second waste outlet arranged to couple with streams of non-target cells at the downstream end of the second spiral channel portion; and a sample outlet arranged to couple with streams of target cells at the downstream end of the second spiral channel portion.

The invention discloses a DC (Dendritic Cell) targeting peptide and application of the targeting peptide. An amino acid sequence of the DC targeting peptide is as shown in SQE ID No:1 or SQE ID No:2 or SQE ID No:3. The DC targeting peptide disclosed by the invention has targeting property and DC enrichment capability; and a tumor specific antigen fused with the DC targeting peptide can be accurately guided and enriched onto the DC surface.

The invention discloses a liquid-based thin-layer cell sheet making device. The liquid-based thin-layer cell sheet making device comprises a sample cup; a detachable filtering head and a detachable sheet making head are arranged on the sample cup sequentially; the sheet making head comprises a sheet making cylinder and a sheet making platform arranged in the sheet making cylinder; a cell precipitating sheet, which is protruded out of the end face of the sheet making platform and is opposite to the filtering head, is arranged on the sheet making platform; an outlet pipe communicated with the filtering head is arranged at the outer end of the sheet making platform; a slide clamping groove positioned between the filtering head and the sheet making platform is arranged on the sheet making cylinder; the cell precipitating sheet is protruded out of the end face, adjacent to the cell precipitating sheet, of the slide clamping groove and can abut against a slide inserted into the slide clamping groove. The liquid-based thin-layer cell sheet making device integrates sampling, filtering and separation, precipitation cell enrichment and cell tissue transfer, the sample cup does not need to bechanged in the process, the sample does not need to be transferred, and liquid-based cell sheet making can be completed only by manually inserting the slide, and the liquid-based thin-layer cell sheet making device is simple in structure, convenient to use and suitable for popularization.

The present invention discloses a circulating tumor cell isolation and enrichment device based on a closed loop. The circulating tumor cell isolation and enrichment device based on the closed loop includes a top plate; two ends of the bottom of the top plate are respectively fixedly connected with a left bracket and a right bracket located at the right side of the left bracket; the left side of the right bracket is fixedly provided with a device main body which is of a hollow structure; the bottom of the device main body is provided with a sample outlet where a liquid collecting bottle is disposed, and a filter membrane is further disposed between a top bottleneck of the liquid collecting bottle and the sample outlet; the inside of the device main body is movably provided with an oscillation plate; and the top of the oscillation plate is fixedly provided with a lifting bar and a toothed bar located at the right side of the lifting bar. In the circulating tumor cell isolation and enrichment device based on the closed loop, the activity of a sample liquid in the device main body may be accelerated, the disadvantage that an original liquid is relatively still and relatively low in flowability and activity is changed, and the isolation of the sample liquid and the filter membrane in a cell enrichment device may be further accelerated, so that the tumor cell isolation and enrichmentefficiencies are greatly increased, and the use demands of people are met.