Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell enrichment and separation method

A separation method and cell technology, applied in the field of cell separation, can solve the problems of target cell purity limitation, low detection sensitivity target cell purity, and detection sensitivity subsequent gene analysis limitations, etc. reduced effect

Active Publication Date: 2017-11-24
GD TECH INC
View PDF11 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Circulating tumor cells (CTC) are important markers for the study of the mechanism of tumor spread and the efficacy of early detection, prevention and clinical treatment of tumors, because they exist in a small number in the blood and require high detection technology, while the current market The technology cannot meet the requirements of the market as a routine detection item in terms of detection sensitivity and reasonable price
Most of the more mature detection technologies on the market use positive magnetic bead enrichment method or negative magnetic bead enrichment method alone, which are limited in detection sensitivity and subsequent gene analysis of cells. For example, a single positive magnetic bead enrichment method The detection sensitivity of the collection method is limited and the purity of the captured target cells is not high, and the single negative magnetic bead enrichment method is also very limited in terms of the purity of the target cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell enrichment and separation method
  • Cell enrichment and separation method
  • Cell enrichment and separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Such as Figure 1a-1b As shown, the cell enrichment and separation method of Example 1 of the present invention includes the following steps:

[0043] Add negative magnetic beads into the biological sample solution tank, the negative magnetic beads are bound to antibodies, the biological samples include tumor target cells and non-target cells to be captured, the antibodies bind to non-target cells; the biological samples are raw blood samples , the above-mentioned antibody is CD45, and the above-mentioned non-target cells include red blood cells and white blood cells;

[0044] Place the capture magnetic head in the biological sample combined with the above-mentioned negative magnetic beads, through the micro-speed scanning movement of the magnetic head in the sample solution, the non-target cells in the sample solution are adsorbed to the magnetic head, and transferred to the collection container; thus , the non-target cells in the above biological sample are separated ...

Embodiment 2

[0049] Such as figure 2 As shown, the cell enrichment and separation method in Example 2 of the present invention includes the following steps:

[0050] Add positive magnetic beads into the biological sample solution tank, the positive magnetic beads are bound to antibodies, the biological samples include tumor target cells and non-target cells to be captured, the antibodies bind to tumor target cells; the biological samples are raw blood samples , the above-mentioned antibody is Muc1 and the above-mentioned non-target cells include red blood cells and white blood cells;

[0051] Place the capture magnetic head in the biological sample combined with the above-mentioned positive magnetic beads, through the micro-speed scanning movement of the magnetic head in the sample solution, the tumor target cells in the sample solution are adsorbed to the magnetic head, and transferred to the collection container; Target cells are collected for counting and cellular and genetic analysis...

Embodiment 3

[0054] This example is similar to Example 1, except that the biological sample is a blood sample lysed from red blood cells; the antibody bound to negative magnetic beads is red blood cell antibody, and the antibody bound to positive magnetic beads is keratin antibody.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a cell enrichment and separation method which comprises the following steps: 1) combining a first kind of magnetic beads with a biological sample, wherein the biological sample comprises a first kind of cells and a second kind of cells, the first kind of magnetic beads are combined with a first kind of antibody, and the first kind of cells are combined with the first kind of magnetic beads; 2) putting a capturing magnetic head in the biological sample combined with the first kind of magnetic beads, and adsorbing the first kind of cells on a capturing magnet device; 3) releasing the first kind of cells from the capturing magnetic head device, and eliminating the first kind of cells in the biological sample; 4) adding a second kind of magnetic beads into the biological sample without the first kind of cells, and combining the second kind of cells with the second kind of magnetic beads; 5) putting the capturing magnetic head in the biological sample combined with the second kind of magnetic beads, and adsorbing the second kind of cells on the capturing magnet device; 6) releasing the second kind of cells from the capturing magnetic head device. The method provided by the invention has high detection sensitivity and purity, and can be used for analyzing multiple kinds of gene markers of single cell or a small amount of cells.

Description

technical field [0001] The invention belongs to the technical field of cell separation, and in particular relates to a cell enrichment and separation method. Background technique [0002] Circulating tumor cells (CTCs) are important markers for the study of the mechanism of tumor spread and the efficacy of early detection, prevention and clinical treatment of tumors, because they exist in a small number in the blood and have high requirements for detection technology, while the current market The technology cannot meet the requirements of routine testing items on the market in terms of detection sensitivity and reasonable price. Most of the more mature detection technologies on the market use positive magnetic bead enrichment method or negative magnetic bead enrichment method alone, which are limited in detection sensitivity and subsequent gene analysis of cells. For example, a single positive magnetic bead enrichment method The detection sensitivity of the collection metho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 邓亚光
Owner GD TECH INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com