Method for high efficiency enrichment culture of microalgae

Abstract

The invention discloses a method for high efficiency enrichment culture of microalgae. According to the invention, an airlift bioreactor and an illuminated airlift bioreactor are in series connection; the airlift bioreactor contains microalgae and a microalgae culture medium, air is introduced to realize airlift and aerobic culture, and a microalgae cell enrichment apparatus is employed in a liquid phase loop; the illuminated airlift bioreactor contains microalgae enriched from the airlift bioreactor and a microalgae culture medium, gas containing CO2 is introduced to realize airlift, supply of CO2 and desorption of O2, and the introduced gas containing CO2 is industrial CO2-containing tail gas which passes through a conveying pretreatment apparatus and then enters into the illuminated airlift bioreactor. Compared with the prior art, the method provided by the invention has the following advantages: high efficiency enrichment of microalgae cells and accumulation of high-content lipid of the microalgae cells are realized, a utilization rate of CO2 is increased, O2 desorption capability is enhanced, culture apparatuses are reduced, etc.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and relates to a method for efficiently enriching algae cells and utilizing CO in the process of cultivating microalgae in a bioreactor 2 A method of achieving grease accumulation. Background technique [0002] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food, medicine and energy; they can accumulate fatty acids in large quantities, and the fatty acid content of some microalgae can account for 30% to 60% of dry weight. The use of microalgae to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. Not only has strong market potential, but also has extraordinary social value. [0003] However, most wild microalgae do not grow fast and have limited ability to fix carbon dioxide. To achieve large-scale production, it will consu...

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Problems solved by technology

[0005] The second is the heterotrophic culture method, which is to provide artificial carbon sources during the culture process, such as glucose. Obviously, glucose is a heterotrophic carbon source, which is a quick-acting carbon source and can increase the growth rate of microalgae, but its oil accumulation efficiency is not high. There is no corresponding improvement, and at the same time, this type of carbon source will inevitably increase the cost of cultivation, which will be greatly limited by raw materials

Its oil accumulation is mainly completed in the heterotrophic stage, and the respiration also consumes organic carbon sources during oil accumulation, and the accumulated oil is also provided by organic carbon sources, so more organic carbon sources are consumed, and the production cost is high. Play the function of microalgae cells to fix carbon dioxide

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