Integrated system for cell enrichment, separation, dyeing and flaking

Abstract

The invention belongs to the field of biological sample analysis and detection, and particularly relates to an integrated system for cell enrichment, separation, dyeing and flaking. The integrated system for cell enrichment, separation, dyeing and faking comprises an automatic control module, and a mechanical control device, a cell separation device and a specimen slide preparation device which can be controlled by the automatic control module in a linkage manner. According to the system disclosed by the invention, CTCs tumor cells are extracted by using the cell separation device in a filtering manner and then transferred to the specimen preparation device for dyeing treatment, and the whole operation process has relatively high automation degree and relatively high flux. All modules ofthe system are reasonable in layout and compact in structure; various functions of sample transferring, sample fixing, sample filtering, cell staining and sample sealing are integrated into one systemfor operation, the whole system is extremely high in integration degree, has the advantages of automation and high flux, can meet the requirement of individualized medical treatment, and is applied to various medical institution occasions, timely diagnosis and the like.

Description

technical field [0001] The invention belongs to the field of analysis and detection of biological samples, and in particular relates to an integrated system for cell enrichment, separation, staining and film production. Background technique [0002] Circulating tumor cells (CTCs) refer to tumor cells that are released from solid tumors or metastases into the peripheral blood circulation spontaneously or due to diagnostic and therapeutic procedures, and are an important cause of postoperative recurrence and distant metastasis in patients with malignant tumors. In recent years, with the continuous improvement of detection technology, CTCs detection, as a new type of non-invasive diagnostic tool, has become the focus of clinical research for its application value in early detection of postoperative recurrence and distant metastasis, evaluation of curative effect and prognosis, etc. hot spot. However, because the number of CTCs in peripheral blood is very small, it is usually n...

AI Technical Summary

Problems solved by technology

[0004] However, in the actual CTCs isolation and enrichment method, in the process of cell separation and staining, the separation efficiency is not high enough due to the slow separation speed of cells, and the sedimentation of cells in the sample can easily lead to filter clogging; in addition, the actual operation found that after cell separation, many non-tumor cells are likely to remain on the back of the filter membrane, which will cause great interference to the reading process after staining; moreover, in the staining process after CTCs enrichment, it is necessary to manually filter The process of drying, sealing and re-drying membrane samples makes the entire CTCs detection process relatively cumbersome and affects work efficiency

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