40 results about "Bovine collagen" patented technology

The invention relates to a collagen temperature-sensitive hydrogel and a preparation method thereof Soft tissue filling materials utilized in the existing plastic and beauty treatment industry have the problems of hardening, production of phyma, too fast absorption speed, and induction of chronic inflammation. American F Dalton approves successively multiple products for soft tissue filling, and main components of the products comprise bovine collagen, hyaluronic acid sodium, polymethyl methacrylate (PMMA) globules and the like. The products have good effects but can still produce cases of redness and allergy of a used position and too fast absorption. In the invention, collagen solution, chitosan acid or hyaluronic acid, and beta-sodium glycerophosphate solution are mixed according to a volume ratio of (1 to 3): (1 to 5): (1 to 2), then are stirred well at a temperature of 0 to 15 DEG C for 10 to 30 minutes and are adjusted to a pH of 7.4 to form temperature-sensitive hydrogel. The temperature-sensitive hydrogel has the advantages of good gel forming property at a temperature of 37 DEG C, gel forming time within 10 minutes, good mechanical strength, long degradation time, low immunological rejection, and favorable safety and effectiveness.

A method of treating volume deficiency in cosmetic surgery and in urological stress incontinence is based upon the transplantation of one or more biologic collagen based tissues into the subcutaneous, deep dermal, or peri urethral space. In the preferred embodiment, mechanically morsellized dehydrated embryonic bovine matrix tissue is used, which is derived from commercially available sheets of lyophilized fetal bovine collagen. The specific morsellization, cubing and hydration techniques described herein allow the percutaneous insertion of the transplanted collagen tissue through a syringe using a needle. Morsellization and percutaneous injection also decreases the insertion requirements of the material transferred, thus allowing the surgeon to insert more material into the desired space with less morbidity. Once in the body, the collagen matrix acts as a scaffold to allow the ingrowth of host human fibroblasts. One or more therapeutic substances may be added, including growth factors, differentiation factors, hydrogels, polymers, antibiotics, anti-inflammatory medications, or immunosuppressive medications. These additional substances may or may not be dehydrated.

Provided are synthetic bone graft substitutes that include bioactive glass and a carrier. Synthetic bone graft substitutes may include bioactive glass, glycerol and polyethylene glycol. Also provided are bone graft substitutes that include collagen and bioactive glass particles. Example bone graft substitutes may include collagen and bioactive glass particles. Other example embodiments may include Type I Bovine Collagen, an angiogenic agent, such as hyaluronic acid, and bioactive glass. Further provided are methods that include administering the present bone graft substitutes to a mammal, e.g., by surgical insertion of the bone graft substitute into the mammal, either alone or in conjunction with one or more implant devices. Further provided are kits that include the present bone grafts.

The invention belongs to the technical field of medical treatment and discloses collagen dressing for promoting would healing. The collagen dressing is prepared from, by weight, 50-70% of bovine collagen oligopeptide, 0.5-5% of a modified chitosan solution, 5-20% of sea cucumber oligopeptide or cecropin B, 1-5% of ginseng oligopeptide, 0-20% of a wetting agent and the balances of solvents. A membrane is formed on the surface of the dressing to achieve antibacterial efficacy, high adhesion force is achieved, and long-acting antibacterial performance is achieved; meanwhile, infection can be isolated, and the novel liquid dressing has the advantages of biocompatibility, safety, antibacterial performance, would healing promotion and the like.

The invention discloses a yak collagen preparation method. The yak collagen preparation method comprises the steps of smashing yak tissue, then carrying out enzymolysis, separating the enzymolytic interstitial fluid and purifying to obtain the collagen solid. Thus, the yak collagen preparation method is simple.

The invention discloses a water and fertilizer conservation attapulgite vegetable cultivation matrix. The raw material of the matrix comprises, by weight, 60-80 parts of attapulgite clay, 10-20 partsof zeolite powder, 6-14 parts of diatomite, 15-25 parts of sepiolite powder, 4-6 parts of calcined kaolin, 3-5 parts of gangue, 2-5 parts of phosphate rock powder, 3-6 parts of quartz sand, 4-10 partsof waterproof binder, 95-105 parts of organic fertilizer, 15-25 parts of coconut shell charcoal powder and 4-8 parts of corn straw. The waterproof binder is prepared by the following processes: bovine collagen, polysorbate, and water are stirred and freeze-dried, sodium carboxymethyl cellulose and acetic acid aqueous solution are added, heated, stirred and freeze-dried to obtain the waterproof binder. The matrix is comprehensive in nutrients, the matrix is loose, the specific surface area is extremely large, the adsorption strength is high, the nutrients are not easy to lose, the shape structure can be effectively maintained, the phenomenon of collapse does not easily occur, the structure characteristic of water and fertilizer conservation is effectively maintained, meanwhile, environmental pollution can be reduced.

The invention discloses a composite collagen membrane for bone grafting in an alveolar bone defect area and a preparation method of the composite collagen membrane, the composite collagen membrane isof a double-layer structure and is composed of a compact collagen fiber membrane layer and a collagen sponge layer; wherein the collagen fiber membrane layer is made of bovine collagen fibers, and thecollagen sponge layer comprises bovine collagen fibers and hyaluronic acid. The compact layer can prevent non-osteoblasts from growing towards a bone grafting area, but allows fibroblasts to be attached along collagenous fibers; and a three-dimensional frame structure of the collagen sponge layer serving as the inner side is beneficial to osteoblast fusion and bone regeneration supporting. The composite collagen membrane has low antigenicity and excellent biocompatibility and has enough barrier time after being used for a human body, and the membrane can keep a complete structure in a wet state and achieve the effect of being attached to the bone wall; after the composite collagen membrane is used for about 1-2 months, the composite collagen membrane starts to be automatically degraded, and is finally decomposed into components which are non-toxic and harmless to a human body and are absorbed by tissues.

Bovine collagen is freed of non-collagen proteins, glycosaminoglycans and lipids by microbial treatment to yield a product, which is undenatured fibre of high tensile strength. The microbial extraction technique ensures degradation of non-collagenous components with the help of the protease combination produced, leaving the collagenous matter intact due to the absence of collagenase in the secreted enzymes. The resulting atelopeptide collagen has largely monomeric triple helical conformation. This process results in the formation of regularly ordered fibres of collagen possessing a rope-like structure. It is soluble in dilute acidic aqueous solutions. The collagen is rendered non-immunogenic by the removal of certain terminal peptide chains. The non-cytotoxic fibres can be fabricated into various physical forms for biomedical applications.

The invention provides a sequential extraction process for bovine collagen peptides, which comprises the following steps: performing primary extraction by adopting ultrasonic waves; performing solid-liquid separation to obtain filtrate and filter residue; performing primary enzymatic hydrolysis to obtain a primary enzymatic hydrolysate; performing enzyme deactivation, separating undissolved substances, and removing impurities to obtain a primary refined solution; performing concentration treatment; performing sterilization and drying to obtain primary protein peptide powder; performing secondary enzymatic hydrolysis to obtain a secondary enzymatic hydrolysate; performing concentration treatment; performing sterilization and drying to obtain secondary protein peptide powder. According to the sequential extraction process for bovine collagen peptides, the primary extraction is performed in an alkalescence environment with the pH value being 7-8, the extraction efficiency is improved under a 0-0.04 Mpa micro-high-pressure environment through matching of the operation temperature being 100-105 DEG C, the collagen peptide purity and the molecule integrity are ensured, and the protein utilization ratio is relatively high; the extraction efficiency (production efficiency) is improved through the combination of ultrasonic wave extraction, and acids, alkalies and other chemical reagents are avoided from use and emission, so that the process is friendly to the environment and does not pollute the environment.

The invention discloses yak collagen peptide and an application thereof. The collagen peptide is high-quality Qinghai yak peptide of 3000 Da or lower, has good anti-aging and anti-oxidation effects, can be directly absorbed by a human body and can achieve good beauty and health care effect, and a beauty and health care product prepared from the collagen peptide has good anti-aging effect.

The invention discloses a production method of a complete peptide chain bovine collagen. The production method is characterized in that the operations are conducted under a low temperature condition after a bovine achilles tendon is pretreated, and the method comprises the following steps: firstly processing by using a non-collagen removing solution, then dissolving by using a collagen dissolvingsolution, supplementing the collagen dissolving solution to continue to dissolve the collagen after fully homogenating, then adding collagen enzymolysis liquid to implement enzymolysis, collecting supernate after centrifuging, then adding a collagen precipitation solution to precipitate the collagen, collecting a precipitate after centrifuging, repeating the processing steps of dissolving the collagen by using the collagen dissolving solution, centrifuging to collect the supernate, precipitating the collagen by using the collagen precipitation solution, centrifuging to collect the precipitate,dissolving by using the collagen dissolving solution and centrifuging to collect the supernate, finally implementing dialysis on the supernate to obtain the complete peptide chain bovine collagen, and storing after freeze-drying. The complete peptide chain bovine collagen can be applied to the preparation of tissue engineering carrier supports, surgical sutures, wound-protecting dressings and thelike as a medical biological material.

The invention discloses an application of a hydro-acupuncture liquid medicine in preparation of a medicine for treating lymphatic obstruction area. The hydro-acupuncture liquid medicine comprise liquid medicine, wherein the liquid medicine comprises the following raw material components in parts by weight: 1 to 3 parts of a medical collagen filling agent, 2 to 6 parts of a Radix Astragali injection, 2 to 6 parts of a compound Chinese angelica injection and 100 to 300 parts of normal saline, wherein the medical collagen filling agent is a normal saline suspension of 3.5% of bovine collagen and0.3% of lidocaine hydrochloride. The liquid medicine is injected into acupuncture points in a lymphatic obstruction area, so that qi and blood circulation is increased, channels and collaterals and lymph are dredged, and stasis toxin is eliminated.

The invention provides a formula and a preparation method of bone peptide composite food, and relates to the technical field of sports food. The formula of the bone peptide composite food comprises the following raw materials in parts by mass: 75 to 100 parts of polydextrose, 68 to 88 parts of bovine collagen peptide powder, 30 to 50 parts of creatine, 30 to 40 parts of milk mineral salt, 0.08 to 0.4 part of tricalcium phosphate, 3 to 8 parts of lindera aggregata leaf powder, 0.1 to 0.8 part of fructo-oligosaccharide, 0.09 to 0.45 part of saussurea involucrata culture powder, 5 to 12 parts of casein phosphopeptide powder, 0.09 to 0.45 part of chitosan oligosaccharide, 0.06 to 0.2 part of magnesium oxide, 0.09 to 0.1 part of 1, 6-fructose diphosphate trisodium salt, 1 to 4 parts of ginseng powder, 2 to 7 parts of rhizoma polygonati powder, 3 to 10 parts of fructus lycii powder, 1 to 8 parts of rhizoma curcumae longae powder, 2 to 6 parts of fructus hippophae powder, 3 to 8 parts of bovine bone peptide powder, 0.08 to 0.12 part of sodium hyaluronate, 0.02 to 0.08 part of vitamin C, 0.04 to 0.06 part of ferric pyrophosphate, 0.02 to 0.05 part of vitamin B6, 0.02 to 0.05 part of vitamin B12, 0.03 to 0.06 part of vitamin D, 0.09 to 0.5 part of citric acid and 0.09 to 0.45 part of stevioside. The bone peptide composite food is high in nutrient and good in eating effect through matching of various substances.

Provided are synthetic bone graft substitutes that include bioactive glass and a carrier. Synthetic bone graft substitutes may include bioactive glass, glycerol and polyethylene glycol. Also provided are bone graft substitutes that include collagen and bioactive glass particles. Example bone graft substitutes may include collagen and bioactive glass particles. Other example embodiments may include Type I Bovine Collagen, an angiogenic agent, such as hyaluronic acid, and bioactive glass. Further provided are methods that include administering the present bone graft substitutes to a mammal, e.g., by surgical insertion of the bone graft substitute into the mammal, either alone or in conjunction with one or more implant devices. Further provided are kits that include the present bone grafts.

The invention discloses a method for excising immunogenicity terminal peptide through pepsin, and belongs to the technical field of peptide treatment. According to the method for excising the immunogenicity terminal peptide through the pepsin, in suspension liquid manufactured by using cowhide as a raw material, terminal amidogen and carboxyl are excised through the pepsin. The method includes the steps that the pepsin is dissolved in acetic acid of 0.5 M to be made into a pepsin solution of 10 mg / ml, then the pepsin solution is transferred into tissue homogenate till the concentration is 0.1 mg / ml, namely 1 ml of the pepsin solution is added into every 100 ml of the homogenate, the mixture is evenly mixed, and digestion is conducted for 7-10 days at the indoor temperature. The method is simple in technology and easy to operate. Through the process, a spherical area containing bovine collagen molecule amidogen and carboxyl terminal (terminal peptide) in the cowhide tissue suspension liquid can be effectively excised, the natural spatial helical segment structure and biological activity of collagen are maintained, and therefore the potential immunogenicity of the bovine collagen is removed.

The invention belongs to the technical field of enzymatic hydrolysis of bovine collagen peptides, and in particular relates to a production process of bovine collagen peptides, including S1 cleaning, S2 denaturation, S3 enzymatic hydrolysis, S4 centrifugation, S5 enzyme inactivation, S6 impurity removal, and S7 finished product. The present invention has the following advantages and effects: the application uses ultrasonic technology, denaturation technology and enzymatic hydrolysis technology to work together, which can be conveniently adapted to cowhide from various origins; and the collagen peptide product produced by the technology provided by the application has good dissolution properties and dispersibility, good sensory quality, high yield, and a large amount of active polypeptides in the product make it have strong antioxidant capacity; the method of this application has relatively mild reaction conditions, low production energy consumption, and far superior process conditions and process effects in existing technical solutions.

The invention discloses a beef tendon rinsing and roasting slice processing method. By adoption of traditional food materials and an advanced enzymatic reaction technique, intrinsic collagen and protein of the raw materials are subjected to cross-linking reaction with substances such as external cattle collagen, then modern slicing equipment is adopted for accurate shaping and slicing, and high collagen content is realized. In addition, beef tendon rinsing and roasting slices processed according to the method is chewy in taste, rich in nutrition, convenient to consume and suitable for cold dishes, rinsing, roasting and the like.

The invention discloses a special composite fertilizer with comprehensive nutrients for lotus. The special composite fertilizer is prepared from the following raw materials in parts by weight: 100 to120 parts of a large amount of element fertilizer, 32 to 45 parts of fermented soybean meal, 10 to 18 parts of decomposed pig manure, 20 to 30 parts of peanut shell powder, 4 to 12 parts of EM (effective microorganism) bacterial agent, 0.1 to 0.4 part of naprosyl sodium, 1 to 3 parts of collagen complex, 0.1 to 0.4 part of microcrystalline cellulose, 2 to 4 parts of zeolite powder, and 2 to 6 parts of trace element additive. The collagen complex is prepared by the following technology with steps of stirring kaolin, a silane coupling agent and anhydrous ethanol, adjusting the pH (potential of hydrogen) value of the system to 5.2 to 5.8, heating and stirring, filtering, washing by the anhydrous ethanol, drying, adding bovine collagen, continuing to stir, removing the anhydrous ethanol, adding sodium carboxymethyl cellulose and acetic acid solution, stirring, and freeze-drying, so as to obtain the collagen complex.

The invention discloses a method for screening characteristic fragments of yak collagen through high-resolution mass spectrometry. The method is characterized by comprising the following steps: (1) sample collection; (2) pretreatment; (3) performing enzymolysis; (4) high performance liquid chromatography separation: separating the enzymatic hydrolysate by adopting a high performance liquid chromatography system EasynLC with a nano-liter flow velocity; (5) mass spectrum identification: carrying out mass spectrum identification by using a Q-Exactive mass spectrometer after carrying out chromatographic separation on the enzymatic hydrolysate; and (6) characteristic fragment screening: retrieving a UniProt protein database in an original file of a mass spectrum identification result by using Mascot2.2 software, and screening to obtain the characteristic fragments of the yak collagen. According to the method, yak collagen subjected to enzymolysis is separated and identified through a high performance liquid chromatography-tandem mass spectrometry technology, data are compared and analyzed through Mascot2.2 software and a UniProt protein database, and yak collagen characteristic fragments which can be detected in an actual sample are screened out. The identified characteristic fragments can be used for detecting the authenticity and reliability of the yak-derived collagen, and an effective technical support is provided for the aspect of yak collagen component supervision.

The invention discloses a method for producing ox collagen by means of tail-end gelatin of oxhide gelatin, and belongs to the field of oxhide processing. According to the method, through steps of ethyl alcohol pre-treatment, enzymolysis, enzyme removal and the like, the collagen is extracted, through ethyl alcohol, pre-treatment can be conducted on the tail-end gelatin easily, impurities in the tail-end gelatin are removed, the purity and the quality of the collagen are improved, enzymolysis time is shortened, by adjusting an enzymolysis sequence, the molecular weight of the collagen is controlled, collagen products with molecular weight between 3 KDa to 8 KDa can be prepared, and the molecular weight of the prepared collagen is centralized, and can account for more than 70% of total molecular weight of collagen; a whole preparation process can be completed in 15-20 h, so that compared with the prior art, the method for producing the ox collagen by means of the tail-end gelatin of theoxhide gelatin has the advantage that the time cost is greatly saved.

The invention discloses a method for producing ox collagen by means of tail-end gelatin of oxhide gelatin, and belongs to the field of oxhide processing. According to the method, through steps of ethyl alcohol pre-treatment, enzymolysis, enzyme removal and the like, the collagen is extracted, through ethyl alcohol, pre-treatment can be conducted on the tail-end gelatin easily, impurities in the tail-end gelatin are removed, the purity and the quality of the collagen are improved, enzymolysis time is shortened, by adjusting an enzymolysis sequence, the molecular weight of the collagen is controlled, collagen products with molecular weight between 3 KDa to 8 KDa can be prepared, and the molecular weight of the prepared collagen is centralized, and can account for more than 70% of total molecular weight of collagen; a whole preparation process can be completed in 15-20 h, so that compared with the prior art, the method for producing the ox collagen by means of the tail-end gelatin of theoxhide gelatin has the advantage that the time cost is greatly saved.

The invention relates to the technical field of animal food processing, in particular to a bovine collagen peptide capable of relieving fatigue and improving bone density and a preparation method thereof. The preparation method of the bovine bone collagen peptide of the present invention comprises the following steps: using bovine bone as a raw material, enzymatically hydrolyzing the pretreated raw material to obtain an enzymatic hydrolyzate; the compound protease is neutral protease, papain and flavor Protease. The bovine bone collagen peptide prepared by the method has a high content of small molecular peptides; it has good water solubility, is easily absorbed by the human body, and has no bad smell; it has good effects of relieving fatigue and improving bone density; and the preparation method is simple and convenient to operate. Industrial production.

The invention relates to the technical field of health care, in particular to beriberi-removing targeted foot soaking liquid for foot care, which comprises a cell repairing solution and a beriberi-removing solution, wherein the cell repairing solution is prepared by mixing 1-3% by weight of ocean deep ion magnesium, 1-3% by weight of fish collagen peptide, 1-3% by weight of bovine collagen peptide with white and 1-3% by weight of whey peptide, and the balance of small molecular group active water is supplemented to dissolve the solutes to form a solution; and the beriberi-removing solution is prepared by mixing the following components in percentage by weight: 0.5-5% of salicylic acid, 0.5-5% of benzoic acid, 0.5-5% of boric acid, 0.15-0.5% of tannic acid, 0.1-0.5% of resorcinol and 0.05-0.15% of furacilin, and the balance of small molecular group active water is supplemented to dissolve the solutes to form the solution. Under the synergist effect of the microcular active water, compounds, peptides and magnesium ions, the beriberi-removing targeted foot soaking liquid can remove various types of beriberi and foot odors thoroughly, remove cutin, inhibit bacteria, protect normal skin tissues of feet, solve the problem of fungal infection fundamentally within a short time, and achieve the purpose of foot health.

The invention belongs to the field of collagen peptide, and discloses bovine bone collagen peptide and a production method thereof. The production method of the bovine bone collagen peptide comprisesthe steps of impurity removal, smashing and cooking on bovine bone, oil-water separation for cooking liquid, high-efficiency compounding of enzymic preparations, enzymolysis on the condition that thepH value is not adjusted and the temperature condition is not controlled, filtering of an inorganic nanofiltration ceramic membrane to remove components which are easily dissolved and components whicheasily absorb moisture, high-temperature and high-pressure treatment, concentration and drying for powder preparation. According to the method, the enzymolysis pH value does not need to be adjusted,the enzymolysis temperature does not need to be controlled, the enzymolysis time is short, the enzyme addition amount is small, and the enzymatic products are low in molecular weight and are centralized; meanwhile, the prepared bovine bone collagen peptide powder is excellent in water re-dissolubility, and does not easily absorb moisture or get caked in the storage process.

The invention discloses a lotus root fertilizer with a high fertilizer utilization rate, containing the following materials in parts by weight: 60-100 parts of ammonium nitrate, 32-55 parts of triplesuperphosphate, 24-35 parts of potash magnesium sulphate fertilizer, 1-3 parts of a microelement additive, 45-66 parts of chicken dung, 20-54 parts of radish leaf, 6-12 parts of lucerne meal, 0.1-0.3parts of composite bacteria liquid, 14-20 parts of a controlled release formulations, 1-2 parts of quartz sand, and 1-3 parts of xanthan gum. The composite bacteria liquid contains nitrogen-fixing bacteria, rhizobium, phosphorus release agent, potassium bacteria, bacillus and actinomycetes, wherein the content of living bacteria is 1-1.8*10<8>CFU / ml. The controlled release formulations include a collagen compound, medical stone powder and shell carbon. The collagen compound is prepared by adopting the following process: blending bovine collagen, rosin and water, performing freeze drying, adding sodium carboxymethyl cellulose and an acetic acid aqueous solution and blending, and performing freeze drying to obtain the collagen compound.

In the present invention, the porcine decellularized dermal matrix is ​​prepared by placing the dermal matrix in a 0.5Mol / L sodium hydroxide solution prepared with triple-distilled water and continuously shaking it, then rinsing it with triple-distilled water and phosphoric acid balanced saline, and freeze-drying , packed and sterilized; pig acellular dermal matrix is ​​used as a scaffold for in vitro culture of epidermal cells to prepare tissue-engineered composite skin. After the dermal matrix is ​​coated with type I bovine collagen dissolved in acetic acid, the pH is adjusted to 7.0-7.4, and then placed successively Soak in phosphate balanced salt solution and serum-free medium, take out the inoculated and passaged epidermal cells, and then place them in CO 2 Serum-free culture in the incubator; application of porcine acellular dermal matrix, involving the construction of tissue-engineered composite skin with porcine acellular dermal matrix as a scaffold and the preparation of tissue-engineered composite skin as a substitute for human skin.