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A kind of production process of bovine collagen peptide

A production process and collagen peptide technology, which is applied in the field of protein peptide preparation and can solve the problems of easy precipitation of solutions and poor water solubility of solid collagen.

Active Publication Date: 2021-07-06
GUANGZHOU TIANQI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this preparation method, a chromatographic purification method is used to separate the collagen liquid, and the obtained solid collagen has poor water solubility, and the solution is prone to precipitation after being dissolved in water.

Method used

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  • A kind of production process of bovine collagen peptide
  • A kind of production process of bovine collagen peptide
  • A kind of production process of bovine collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 A kind of production technology of bovine collagen peptide

[0032] The production technology of described bovine collagen peptide, comprises the steps:

[0033] S1. Pretreatment of raw materials: select freshly washed bovine bones, add 10 times the mass of sodium hydroxide solution with a concentration of 0.05 mol / L and soak for 3 hours, then use a bone crusher to crush the bones to 1 cm in size, and put them into steaming Add water that is 8 times the weight of beef bones into the tank, and cook at 120°C for 1.5 hours to obtain a cooking liquid;

[0034] S2. Separation of oil and water: performing oil-water separation on the cooking liquid described in step S1 to remove grease to obtain a crude collagen solution;

[0035] S3, enzymatic hydrolysis: add compound protease to the crude collagen solution obtained in step S2 and carry out an enzymolysis at a temperature of 55° C., the enzymolysis time is 1 h, and the compound protease is bromelain and pepsin i...

Embodiment 2

[0040] Embodiment 2 A kind of production technology of bovine bone collagen peptide

[0041] The production technology of described bovine collagen peptide, comprises the steps:

[0042] S1. Raw material pretreatment: select freshly washed bovine bones, add 15 times the mass of sodium hydroxide solution with a concentration of 0.01mol / L and soak for 4 hours, then crush them to 1.5cm bone pieces with a bone crusher, put them in Add water 10 times the weight of beef bones into the cooking tank, and cook at 130°C for 2.5 hours to obtain a cooking liquid;

[0043] S2. Separation of oil and water: performing oil-water separation on the cooking liquid described in step S1 to remove grease to obtain a crude collagen solution;

[0044] S3. Enzymolysis: add a compound protease to the crude collagen solution obtained in step S2 to perform an enzymolysis at a temperature of 58° C., and the enzymolysis time is 1.5 h. The compound protease is subtilisin, pepsin and trypsin According to t...

Embodiment 3

[0049] Embodiment 3 A kind of production technology of bovine bone collagen peptide

[0050] The production technology of described bovine collagen peptide, comprises the steps:

[0051] S1. Raw material pretreatment: select freshly washed bovine bones, add 20 times the mass of sodium hydroxide solution with a concentration of 0.15 mol / L and soak for 6 hours, then use a bone crusher to crush the bones to 2 cm, and put them into steaming Add water 12 times the weight of beef bones into the tank, and cook at 135°C for 3 hours to obtain a cooking liquid;

[0052] S2. Separation of oil and water: performing oil-water separation on the cooking liquid described in step S1 to remove grease to obtain a crude collagen solution;

[0053] S3. Enzymolysis: add a compound protease to the crude collagen solution obtained in step S2 to carry out an enzymolysis at a temperature of 60° C., and the enzymolysis time is 2 hours. The compound protease is subtilisin, bromelain, pepsin, One or mor...

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PUM

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Abstract

The invention belongs to the technical field of protein peptide preparation, and in particular relates to a production process of bovine bone collagen peptide. The production process of the bovine collagen peptide provided by the present invention includes steps such as S1, raw material pretreatment; S2, oil-water separation; S3, enzymatic hydrolysis; S4, microwave enzyme inactivation; S5, purification; The bovine collagen peptide powder prepared by the bovine collagen peptide production process provided by the present invention has the advantages of small molecular weight, concentrated distribution, fast absorption, low fat content, etc., and has good water solubility, and is not easy to produce precipitation when dissolved in water. High utilization, long-term use can effectively improve human immunity, relieve physical fatigue, and can be widely used in fields such as health food, formula food and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of protein peptide preparation, and in particular relates to a production process of bovine bone collagen peptide. Background technique [0002] Collagen is an extracellular protein, which is a fibrous protein twisted into a helical shape by three peptide chains. Collagen is the most abundant protein in the human body, accounting for more than 30% of the total protein in the body. Collagen is rich in amino acids such as glycine, proline, and hydroxyproline that the human body needs. Collagen is the most important component of the extracellular matrix. There are about 3 kilograms of collagen in the body of an adult, which mainly exists in the skin, bones, eyes, teeth, tendons, heart, stomach, intestines, blood vessels and other parts of the human body. Its function is to maintain the shape and structure of skin, tissues and organs , is also an important raw material for repairing various damaged tissues. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K14/78C12P21/06
Inventor 谢涛王中振谢骞刘家生
Owner GUANGZHOU TIANQI BIO TECH
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