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A method for producing bovine collagen by utilizing cowhide gelatin tail glue

A technology of bovine collagen and gelatin, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problem that the quality of collagen is greatly affected, the practical application of collagen is limited, and the mechanical properties and stability of collagen are poor. And other issues

Active Publication Date: 2020-07-07
山东恒鑫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction of general collagen mainly adopts methods such as acid dissolution, enzymatic hydrolysis, and salting out. During the enzymatic hydrolysis process, trypsin, papain, pepsin, bromelain, etc. are often used. The quality of the collagen has a great influence. The main problems of the current method are that the extraction efficiency of the collagen is not high, the mechanical properties and stability of the collagen are poor and the purity is low, which will seriously limit the practical application of the collagen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of method utilizing cowhide gelatin tail glue to produce bovine collagen, described method comprises the following steps:

[0028] (1) Dissolve 100 g of the endoglucan prepared by the alkaline process in 250 g of ethanol solution with a mass fraction of 2%, filter and remove the solution after stirring for 1.5 h, cool to 5-10° C., let stand for 1-2 h, filter, Rinse with water until no ethanol exists in the filtrate

[0029] (2) Add water to the endo gum treated in step (1) so that the gelatin mass fraction is 8%, after stirring evenly, adjust the pH of the gelatin solution to be 6.5, add 20000U / g 明胶 trypsin, hydrolyze at 50°C for 0.5h;

[0030] (3) Add acetic acid to adjust the pH value of the solution obtained in step (2) to 4.0, then add 20000U / g 明胶 Pepsin hydrolysis for 1h, then add 20000U / g 明胶 Bromelain hydrolyzed for 0.5h;

[0031] (4) Centrifuge the enzymolyzate obtained in step (3), take the precipitate, dissolve it with 0.1% acetic acid by mass fracti...

Embodiment 2

[0035] A kind of method utilizing cowhide gelatin tail glue to produce bovine collagen, described method comprises the following steps:

[0036] (1) Dissolve 100 g of endodontic gum prepared by the alkaline process in 300 g of ethanol solution with a mass fraction of 3%, stir for 1.5 h and filter to remove the solution, cool to 5-10 ° C, let stand for 1-2 h, filter, Rinse with water until no ethanol exists in the filtrate

[0037] (2) Add water to the endo gum treated in step (1) so that the gelatin mass fraction is 6%, after stirring evenly, adjust the pH of the gelatin solution to be 7.0, add 20000U / g 明胶 trypsin, hydrolyze at 45°C for 2.0h;

[0038] (3) Add acetic acid to adjust the pH value of the solution obtained in step (2) to 3.0, then add 15000U / g 明胶 Pepsin hydrolysis for 2h, then add 20000U / g 明胶 Bromelain hydrolyzed for 1.0h;

[0039] (4) Centrifuge the enzymatic hydrolyzate obtained in step (3), take the precipitate, dissolve it with 0.12% acetic acid by mass fra...

Embodiment 3

[0043] A kind of method utilizing cowhide gelatin tail glue to produce bovine collagen, described method comprises the following steps:

[0044] (1) Dissolve 100 g of endodontic gum prepared by the alkaline process into 400 g of ethanol solution with a mass fraction of 4%, filter and remove the solution after stirring for 1.5 h, cool to 5-10° C., let stand for 1-2 h, filter, Rinse with water until no ethanol exists in the filtrate

[0045](2) Add water to the endo gum treated in step (1) so that the gelatin mass fraction is 8%, after stirring evenly, adjust the pH of the gelatin solution to be 6.5, add 30000U / g 明胶 trypsin, hydrolyze at 40°C for 2h;

[0046] (3) Add acetic acid to adjust the pH value of the solution obtained in step (2) to 4.0, then add 10000U / g 明胶 pepsin hydrolysis for 2.5h, then add 10000U / g 明胶 Bromelain hydrolyzed for 2h;

[0047] (4) Centrifuge the enzymolyzate obtained in step (3), take the precipitate, dissolve it with 0.1% acetic acid by mass fractio...

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Abstract

The invention discloses a method for producing ox collagen by means of tail-end gelatin of oxhide gelatin, and belongs to the field of oxhide processing. According to the method, through steps of ethyl alcohol pre-treatment, enzymolysis, enzyme removal and the like, the collagen is extracted, through ethyl alcohol, pre-treatment can be conducted on the tail-end gelatin easily, impurities in the tail-end gelatin are removed, the purity and the quality of the collagen are improved, enzymolysis time is shortened, by adjusting an enzymolysis sequence, the molecular weight of the collagen is controlled, collagen products with molecular weight between 3 KDa to 8 KDa can be prepared, and the molecular weight of the prepared collagen is centralized, and can account for more than 70% of total molecular weight of collagen; a whole preparation process can be completed in 15-20 h, so that compared with the prior art, the method for producing the ox collagen by means of the tail-end gelatin of theoxhide gelatin has the advantage that the time cost is greatly saved.

Description

technical field [0001] The invention relates to a method for producing bovine collagen by utilizing bovine hide gelatin tail gum, and belongs to the field of bovine hide processing. Background technique [0002] Collagen is an extracellular protein consisting of three peptide chains twisted into a helical fibrous protein. Collagen is the most abundant protein in the human body, accounting for more than 30% of the total protein in the body. Collagen is rich in amino acids, of which essential amino acids account for 15.78% of the total amino acids, and protein has high nutritional value. Collagen products have been widely used in food, health food, cosmetics, medicine and other fields, and the market demand has increased sharply, so the extraction process of collagen has attracted more and more attention. [0003] At present, the extraction of general collagen mainly adopts methods such as acid dissolution, enzymatic hydrolysis, and salting out. Trypsin, papain, pepsin, brom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/30
CPCC07K14/78C12P21/06
Inventor 陈庆成
Owner 山东恒鑫生物科技有限公司
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