30 results about "Balance salt" patented technology

The invention provides a poliomyelitis inactivated vaccine and a production method thereof. The poliomyelitis inactivated vaccine is produced by applying a basket-type bioreactor and adopting Vero cells as a matrix. The production method comprises the following specific steps of adding an M199 culture medium into the basket type reactor, inoculating the Vero cells for culture, and after a compact monolayer is grown, washing and changing with Earle's balance salt solution; after the washing and the changing are finished, injecting M199 culture medium solution, inoculating poliomyelitis virus with virus inoculation MOI being 0.01-0.3, further culturing with the temperature being 33+ / -0.5 DEG C, dissolving oxygen by 40-80%, controlling a pH value of a system at the virus culture stage to 7.4-7.6; and after inoculation, culturing for 42-72 hours, thereby obtaining virus solution. The poliomyelitis inactivated vaccine and the production method provided by the invention have the advantages that the proper culture medium is selected and the conditions of proper culture temperature, pH value, inoculating proportion and dissolved oxygen and the like are controlled, so that the virus titer is high, the antigen content is high, the height for differences between batches can be controlled and the mass is uniform.