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Virus delivery culture medium, and preparation method and application thereof

A culture medium and virus technology, applied in the field of culture liquid, can solve the problems of high price, uneven performance, etc., and achieve the effects of low production cost, maintaining integrity, and simple preparation method.

Active Publication Date: 2019-11-12
GUANGZHOU KINGMED DIAGNOSTICS CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to address the problems of high price and uneven performance of the existing virus delivery medium, and provide a virus delivery medium with the advantages of broad-spectrum antibacterial, slowing down virus degradation, and maintaining virus activity

Method used

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  • Virus delivery culture medium, and preparation method and application thereof
  • Virus delivery culture medium, and preparation method and application thereof
  • Virus delivery culture medium, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A virus transport culture medium is prepared by the following method:

[0036] 1) Prepare 4× Hank’s balanced salt solution according to the following formula:

[0037]

[0038] 2) Prepare the virus delivery medium according to the table below:

[0039] Table 1. The virus delivery medium preparation table of embodiment 1

[0040] component name stock solution concentration Dosage Final concentration Hank's Balanced Salt Solution 4× 125mL 0.5× gelatin 5％ 20mL 1g / L BSA 10％ 25mL 2.5g / L sucrose 5％ 20mL 1g / L L-cysteine 0.015mmol / mL 6.6mL 0.1mM / L L-glutamic acid 0.015mmol / mL 6.6mL 0.1mM / L Hepes 1M / L 5mL 5mM / L Amphotericin B 2.5mg / mL 4mL 10mg / L Polymyxin B 2mg / mL 5mL 10mg / L Gentamicin 10mg / mL 1mL 10mg / L water / Set volume to 1L /

[0041] Among them, 4 × Hank's balanced salt solution, sucrose, BSA solution, L-cysteine, L-glutamic acid, Hepes,...

Embodiment 2

[0043] A virus delivery medium, the difference from Example 1 is that the components and proportioning ratio are shown in the following table:

[0044] Table 2. The virus delivery medium preparation table of embodiment 2

[0045] component name stock solution concentration Dosage (Preparation 1L) Final concentration Hank's Balanced Salt Solution 4× 250mL 1× gelatin 5％ 50mL 2.5g / L BSA 10％ 50mL 5g / L sucrose 5％ 50mL 2.5g / L L-cysteine 0.015mmol / mL 10mL 0.150mM / L L-glutamic acid 0.015mmol / mL 10mL 0.150mM / L Hepes 1M 10mL 10mM / L Amphotericin B 2.5mg / mL 8mL 20mg / L Polymyxin B 2mg / mL 10mL 20mg / L Gentamicin 10mg / mL 2mL 20mg / L water / Set volume to 1L /

Embodiment 3

[0047] A virus delivery medium, the difference from Example 1 is that the components and proportioning ratio are shown in the following table:

[0048] Table 3. Example 3 virus delivery medium preparation table

[0049] component name stock solution concentration Dosage (Preparation 1L) Final concentration Hank's Balanced Salt Solution 4× 500mL 2× gelatin 5％ 100mL 5g / L BSA 10％ 100mL 10g / L sucrose 5％ 100mL 5g / L L-cysteine 0.015mmol / mL 20mL 0.3mM / L L-glutamic acid 0.015mmol / mL 20mL 0.3mM / L Hepes 1M 20mL 20mM / L Amphotericin B 2.5mg / mL 16mL 40mg / L Polymyxin B 2mg / mL 20mL 40mg / L Gentamicin 10mg / mL 4mL 40mg / L water / Set volume to 1L /

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Abstract

The invention provides a virus delivery culture medium, and a preparation method and application thereof, and relates to the field of culture solutions. The virus delivery culture medium is prepared from the following raw materials: a Hank's balance salt, hydrophilic glue, serum albumin, disaccharide, an amino acid, a biological buffer agent and an antibiotic. According to the virus delivery culture medium, and the preparation method and application thereof, tea products have the advantages of broad-spectrum antisepsis, virus degradation slowing down, virus activity maintenance and low preparation costs.

Description

technical field [0001] The invention relates to the field of culture fluid, in particular to a virus delivery medium and its preparation method and application. Background technique [0002] Changes in factors such as the environment may cause respiratory viruses to become active, posing a greater threat to human health. At present, people have realized the importance of etiological detection in the diagnosis and treatment of sudden infectious diseases, the demand for clinical virus detection is increasing, and the demand for virus delivery medium is also growing rapidly. [0003] Viruses are composed of nucleic acid molecules and proteins or only proteins. Viruses are small and simple in structure. The virus cannot replicate itself, but invades the host cell with genes, and replicates new viruses with the help of the host cell's replication system. Virus samples need to be collected before virus detection. After collection, the virus samples are stored and transported in ...

Claims

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Application Information

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IPC IPC(8): C12N7/00
CPCC12N7/00
Inventor 刘勇陈建波杨子峰韩琦伍时冠吴秋保
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT
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