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Adding liquid for storing platelets under liquid low temperature condition and application thereof

A technology of platelets and additive solution, which is applied in the fields of application, preservation of human or animal body, animal husbandry, etc., can solve the problems such as the advent of platelet refrigeration additive solution

Active Publication Date: 2013-10-09
SHANGHAI BLOOD CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of in vitro experiments prove that it can improve the viability of platelets stored in cold storage at 4°C, but it has not been able to further evaluate the phenomenon of rapid phagocytosis and clearance of platelets caused by cold storage after reinfusion into the body
Moreover, if these antiplatelet drugs are directly infused into patients without washing, certain side effects will inevitably occur.
Therefore, so far there is no platelet refrigerated supplement that can be used for clinical transfusion to come out

Method used

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  • Adding liquid for storing platelets under liquid low temperature condition and application thereof
  • Adding liquid for storing platelets under liquid low temperature condition and application thereof
  • Adding liquid for storing platelets under liquid low temperature condition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Prepare platelet additive solution according to the formula in Table 1.

[0036] The preparation method is as follows: taking blood anticoagulant, platelet metabolism substrate, balance salt, and cold storage protection agent according to the formula, adding ion water, fully stirring and dissolving, and then filter sterilization or high-pressure steam sterilization.

[0037] Table 1 (unit mmol / L)

[0038] composition

[0039]

Embodiment 2

[0041] Under aseptic conditions, each unit of apheresis platelet sample was divided into two parts, which were placed in special platelet storage bags, centrifuged (1000xg, 15min), and 35% of plasma was retained, and the refrigerated supplement solution 1 ( Contains trehalose and mannitol, the formula is shown in Table 2) and PAS-IIIM (SSP+, Macopharma Inc. has been clinically used for platelet storage at 22°C in Europe) platelet supplement solution, and stored at 4°C for 7 days.

[0042] Table 2.

[0043] composition

Concentration (mmoL / L)

Sodium chloride

69

sodium citrate

10

Sodium dihydrogen phosphate

26

Disodium phosphate

26

potassium chloride

5

magnesium chloride

1.5

sodium acetate

30

Trehalose

50

Mannitol

80

[0044] 1. Platelets were stored for 7 days, and samples were taken, and the platelet count and average platelet volume were m...

Embodiment 3

[0051] Under aseptic conditions, each unit of apheresis platelet sample was divided into two parts, which were placed in special platelet storage bags, centrifuged (1000xg, 15min), and 35% of plasma was retained, and the refrigerated supplement solution 2 ( Contains DMSO and mannitol, the formula is shown in Table 4) and PAS-IIIM (SSP+, Macopharma Inc. has been clinically used for platelet preservation at 22°C in Europe) platelet supplement solution, and stored at 4°C for 7 days.

[0052] Table 4.

[0053] composition

Prescription amount (mmoL / L)

Sodium chloride

69

sodium citrate

10

Sodium dihydrogen phosphate

26

Disodium phosphate

26

potassium chloride

5

magnesium chloride

1.5

sodium acetate

30

DMSO

5％

Mannitol

80

[0054] Platelets were stored for 7 days and samples were taken, and the platelet count and mean platelet volume were measure...

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PUM

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Abstract

The invention discloses an adding liquid for refrigerating platelets, comprising a blood anticoagulant, platelets metabolic substrates, balancing salt and a platelets refrigerating protection agent. The adding liquid for refrigerating the platelets in the invention can reduce refrigeration injury of the platelets under low temperature condition, keep activity of the platelets, meet requirements of intravenous infusion, and can be applied for mass refrigeration of a blood bank.

Description

technical field [0001] The invention relates to a preparation for preserving platelets, in particular to an additive solution for preserving platelets under liquid low temperature conditions. Background technique [0002] Due to the significant risk of contamination by bacteria and other pathogens in the routine storage method of clinical platelets (shaking storage at 22°C), the current storage period of platelets is limited to 5 days. In addition, this condition also leads to the production of various cytokines, such as TGF-β, RANTES, IL-1β, and IL-8, by residual white blood cells in platelet products, which can cause non-hemolytic transfusion reactions and other transfusion side effects . At present, the methods to solve this problem mainly include contamination detection of platelet products, freezing or refrigerated storage, etc. However, previous studies have shown. Low-temperature refrigeration can lead to the activation and damage of platelets, and the platelets wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 谢如锋钱开诚杨洁任亚娜范华骅张晰
Owner SHANGHAI BLOOD CENT
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