The invention discloses an integrated
chip system for high-
throughput sorting and counting detection of
biological particles, and an application. The
chip system comprises a main
microfluidic chip, a
micropipe, a sample liquid
micropump, an exchange liquid
micropump, a first waste liquid collecting device, a second waste liquid collecting device, a third waste liquid collecting device,
laser emitters,
photoelectric conversion devices, optical fibers and a computer, wherein the main
microfluidic chip comprises an asymmetric curved flow path, a first
branch channel, a second
branch channel, a third
branch channel, a main flow path, a branch flow path, aligning marks, etc. The
system utilizes the asymmetric curved flow path to realize pre-focusing and sorting for the particles, utilizes a liquid changing flow channel to realize change of a carrier liquid of to-be-tested particles and particle cleaning, and utilizes a viscoelastic effect and an
inertial effect of a
viscoelastic fluid to realize focus of single equilibrium position of section centers of the particles. The system does not need a sheath liquid, complex pre-cleaning of the particles, and
optical alignment, has advantages of high speed, high precision, integration,
miniaturization,
automation, low cost, simple production process, easy
batch production, etc.