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35results about How to "Realize the detection purpose" patented technology

Nucleic acid amplification system and freeze-drying protective agent thereof

The invention discloses a nucleic acid amplification system and a freeze-drying protective agent thereof. The nucleic acid amplification system comprises a buffer solution, an enzyme mixing solution, a nucleic acid amplification reaction solution and the low-temperature freeze-drying protective agent. The buffer solution further comprises one or more of dNTP, Mgcl2, Thris-Hcl and KCl. The enzyme mixing solution further comprises one or more of RNA reverse transcriptase, RAN inhibitor and DNA polymerase. A preparation method for the freeze-drying protective agent applied to the nucleic acid amplification system comprises the following step that the components are mixed to be uniform according to the adopted concentration ratio to obtain the freeze-drying protective agent. An RNA / DNA fluorescent quantitation nucleic acid diagnostic reagent system can be detected in a freeze-drying mode, freeze drying can be achieved, the detection purpose can be achieved only through one-step hydration, and the purposes of normal temperature transportation and storage which cannot be achieved by a nucleic acid diagnostic reagent at the present stage can be achieved.
Owner:江苏正大天创生物工程有限公司

Wireless sensor network DOS (disk operating system) attack lightweight detection method based on random forest

The invention provides a wireless sensor network DOS (disk operating system) attack lightweight detection method based on a random forest, and relates to the field of wireless sensor network information security. The method includes the steps: collecting one group of monitoring data by wireless sensor network monitoring nodes every other a fixed time, and transmitting the monitoring data to a basestation; generating detection characteristics (A) and (B) according to perception data attack characteristics of DOS attack on the base station, and standardizing the detection characteristics (A) and (B) into (A*) and (B*); combining a training set with the detection characteristics (A*) by a k-means++ algorithm to obtain normal data and noise data, and adding a certain attack data into the normal data obtained at the stage to train the random forest; firstly judging each sample to be detected is noise or not according to the detection characteristics (A*), and detecting whether DOS attack happens or not by the aid of a random forest and the detection characteristics (B*) if not. The method is implemented on the base station, DOS attack is detected without additional contribution of themonitoring nodes, and node calculation, storage and energy are saved.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Method for measuring antigen protein in serum by chemiluminescence protein chips and kit

The invention relates to a method for measuring antigen protein in serum by chemiluminescence protein chips and a kit, which belongs to the medical detection technology. The method is characterized in that a chemiluminescence technology, a standard curve and a protein chip technology are combined for performing quantitative determination of certain antigen protein substance in serum, the high specialty and sensitivity of the chemiluminescence technology and the standard curve are employed for realizing the quantitative determination; the protein chip technology can realize simultaneous detection and analysis of several samples, so that the detection time consumption of a whole set ofe sample is greatly reduced, and the method has the characteristics of reliability, accuracy and low cost; and the method is suitable for general analysis of common antigen protein level in serum and quantitative analysis of great disease antigen marks, and the method is an medical auxiliary detection method having great meaning. The invention also provides a matched kit based on the method.
Owner:BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV

Freeze-drying protective agent applied to nucleic acid amplification system

The invention discloses a freeze-drying protective agent applied to a nucleic acid amplification system. The freeze-drying protective agent is prepared from one or more of trehalose and water treated through diethylpyrocarbonate. The freeze-drying protective agent applied to the nucleic acid amplification system further comprises cane sugar and / or lactose. A preparing method of the freeze-drying protective agent applied to the nucleic acid amplification system includes the following steps that the components are mixed accordingly the use concentration ratio to be even, and the freeze-drying protective agent is obtained. By means of the freeze-drying protective agent, the fluorescence quantitative nucleic acid diagnostic reagent system of RNA / DNA can be detected in a freeze-drying mode, freeze drying can be achieved, the detecting aim can be achieved only through one-step hydration, and the normal-temperature transportation aim and the normal-temperature storage aim which can not be achieved through a nucleic acid diagnostic reagent at the present stage can be achieved.
Owner:江苏正大天创生物工程有限公司

Glass bottle defect detection apparatus and image splitting device

The invention discloses a glass bottle defect detection apparatus and an image splitting device. The glass bottle defect detection apparatus comprises a plane light source, a polarizer, a diaphragm, the image splitting device, a lens and a camera, wherein the polarizer is arranged in front of the plane light source, the diaphragm is arranged in front of the polarizer, a to-be-detected glass bottle is located between the polarizer and the diaphragm, the image splitting device, the lens and the camera are coaxially arranged in front of the diaphragm and are increasingly far away from the diaphragm, the image splitting device comprises a Fresnel biprism, two obliquely intersecting incident planes of the Fresnel biprism face the diaphragm and are arranged to be corresponding to to-be-detected positions, an intersecting prism ridge of the two incident planes is perpendicular to a horizontal plane, a left or right half part of an emergent plane of the Fresnel biprism is plated with a polarizing film, and the polarization direction of the polarizing film is perpendicular to the polarization direction of the polarizer. According to the invention, the detection apparatus can realize detection of conventional and uneven stress defects of a glass bottle only by using one camera, is capable of measuring boundary dimension of the glass bottle and has the advantages of low detection cost, a small occupation space, high accuracy and a low false drop rate.
Owner:潍坊天恒检测科技有限公司

Cooking utensil, detection method and device of inner pot of cooking utensil, storage medium and processor

ActiveCN109717753AImprove securitySolve the technical problem of high detection costWarming devicesProcess engineeringCurrent time
The invention discloses a cooking utensil, a detection method and device of an inner pot of the cooking utensil, a storage medium and a processor. The detection method of the inner pot of the cookingutensil comprises the steps that the cooking utensil is controlled to perform heating according to a first preset heating mode, and a first temperature of the top of the inner pot of the cooking utensil and a first cooking time of the cooking utensil are obtained, wherein the first cooking time is from the time that the cooking utensil is controlled to start heating according to a first preset power to the current time; when it is judged that the first temperature is greater than or equal to a first preset temperature, or the first cooking time is greater than or equal to a first preset time,a second temperature of the bottom of the inner pot of the cooking utensil is obtained; whether or not the second temperature is less than a second preset temperature is judged; under the situation that the second temperature is less than the second preset temperature, it is determined that the type of the inner pot is the first type. The cooking utensil, the detection method and device of the inner pot of the cooking utensil, the storage medium and the processor solve the technical problem that the detection cost for detecting the inner pot of the cooking utensil is high in the prior art.
Owner:ZHEJIANG SHAOXING SUPOR DOMESTIC ELECTRICAL APPLIANCE CO LTD

Solid electrochemical luminescence sensor for detecting mercury ions and preparation method and application of solid electrochemical luminescence sensor for detecting mercury ions

The invention discloses a solid electrochemical luminescence sensor for detecting mercury ions and a preparation method and application of the solid electrochemical luminescence sensor for detecting the mercury ions. The solid electrochemical luminescence sensor is characterized by being a glassy carbon electrode, wherein the surface of the glassy carbon electrode is immobilized with electrically polymerized ABEI, and then the glassy carbon electrode is assembled with glutaraldehyde, DNA1, T-Hg<2+>-T mismatched DNA2 and biotin. The preparation method comprises the steps: preparing the glassy carbon electrode immobilized with the DNA1 and assembling the solid electrochemical luminescence sensor. The solid electrochemical luminescence sensor has the advantages of high sensitivity, high stability, high selectivity, high repetitiveness, easiness in operation and the like, and reagents can be saved.
Owner:乐陵市金山电器有限公司

Lung adenocarcinoma exosome specific miRNAs, and target gene and applications thereof

The invention provides lung adenocarcinoma exosome specific miRNAs, and a target gene and applications thereof. High flux sequencing technology is combined with systematic bioinformatics analysis methods, the specific high-content exosome miRNAs related with lung adenocarcinoma is obtained through screening; the RNA sequences of the lung adenocarcinoma exosome specific miRNAs are represented by SEQ ID No.1-3. It is found that the common target gene of the miRNAs is FOXN3. The target gene is capable of inhibiting fibroblast migration capability, reduction of the expression amount of the targetgene is capable of promoting entering of fibroblast into tumor tissues, and promoting tumor growth. The lung adenocarcinoma exosome specific miRNAs and the target gene thereof can be taken as lung adenocarcinoma diagnosis markers and can be used for preparation of diagnostic kits. An inhibitor of the miRNAs or an expression promoter of the target gene can be used for preparing medicines used for treating lung adenocarcinoma, or medication guidance in treatment of lung adenocarcinoma; and the clinical application value is relatively high, and the application prospect is promising.
Owner:BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION

Method for detecting arc extinguishing performance of circuit breaker

InactiveCN110346715AJudging the arc extinguishing performanceRealize the detection purposeCircuit interrupters testingPower gridElectric power
The invention belongs to the technical field of electrical equipment detection, and particularly relates to a method for detecting arc extinguishing performance of a circuit breaker. A large number ofcircuit breakers are in a power grid, but the arc extinguishing methods of different types of circuit breakers are different, and a general method for detecting the arc extinguishing performance of the circuit breakers is needed. The invention provides a method for detecting arc extinguishing performance of a circuit breaker. The arc extinguishing performance of the circuit breaker is judged according to the electromagnetic wave signal and the arc duration time by detecting the electromagnetic wave signal radiated by a switch arc generated in the switching-on and switching-off process of thecircuit breaker. The method for detecting the arc extinguishing performance of the circuit breaker is wide in application range, and can achieve the detection purpose for various types of circuit breakers; and the arc extinguishing performance of the circuit breaker is judged by acquiring electromagnetic wave signals of the circuit breaker in the states of putting into operation, different operation years and reaching the service life, and the actual production requirement in the power industry is met.
Owner:YUNNAN POWER GRID CO LTD ELECTRIC POWER RES INST

Unsupervised video anomaly detection method and device based on mask auto-encoder

The invention relates to an unsupervised video anomaly detection method and device based on a mask auto-encoder, and the method comprises the steps: carrying out the target detection of each frame of obtained monitoring video data, and extracting the image blocks of the corresponding positions of a detected foreground target in a current frame and a plurality of adjacent frames; constructing cubes according to the image blocks of each frame, and performing interval mask operation on each cube in a time domain; according to the space-time cubes and the optical flow cubes after the interval mask operation, prediction blocks of masked parts in the space-time cubes and the optical flow cubes are obtained through visual network prediction; respectively calculating the prediction loss of each space-time cube and each optical flow cube at the pixel level; and according to the prediction loss of each space-time cube and each optical flow cube, calculating to obtain an abnormal value score of each frame in the monitoring video data, and obtaining an abnormal event detection result of the video frame to which the abnormal value score belongs. And high-performance end-to-end video abnormal event detection is realized.
Owner:NAT UNIV OF DEFENSE TECH

Method for acquiring pathological data, pathology identification method and computer equipment

The invention provides a method for acquiring pathological data, a pathology identification method and computer equipment. The method for acquiring pathological data comprises the steps of acquiring apathological image sample of a to-be-detected part; according to a standard pathological report, searching a pathological position in the pathological image sample; and performing reverse tracing onthe pathological part in an electronic endoscope according to the pathological characteristic which corresponds with the pathological position. The invention further relates to a pathology identification method which comprises the steps of acquiring pathological data, performing training according to the pathological data by means of an artificial intelligent deep learning network, and obtaining astandard pathology identification model; and identifying the pathological data of the new to-be-detected part, thereby determining a pathology condition. The invention further relates to computer equipment which comprises a processor, a memory and a computer application which is stored in the memory and can operate in the processor. Through the method, the pathology identification method and thecomputer equipment, the pathological part can be accurately searched in the electronic endoscope, thereby greatly improving pathology detection probability in advance.
Owner:BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV

Detection apparatus based on time domain analysis and detection method thereof

The invention relates to the detection technology field of a signal acquisition device and especially relates to a detection apparatus based on time domain analysis. The apparatus comprises a standard microphone, a microphone to be detected, a comparison unit, a calculating unit and an AD conversion unit. A standard time domain waveform signal and an acquisition time domain waveform signal are acquired respectively. The comparison unit carries out subtraction processing on the standard time domain waveform signal and the acquisition time domain waveform signal so as to acquire a comparison time domain result. The comparison time domain result is formed by a variable and a constant. The calculating unit calculates the comparison time domain result, the constant is removed, the variable is maintained and statistics is performed on a variable portion so that a distortion degree of the variable is acquired. The distortion degree of the variable is a distortion degree of the microphone to be detected. In the invention, collected time domain signals are directly compared, the signals do not need to be converted into frequency domain signals, operand is small and detection efficiency is high. Simultaneously, a position of the microphone to be detected does not need to be arranged deliberately and a detection purpose can be realized under any positions.
Owner:ZILLTEK TECH SHANGHAI +1

Microelectrode, probe containing microelectrode, and use thereof in measurement of oxygen content in brain

The invention provides a microelectrode, a probe containing the microelectrode, and a use thereof in the measurement of an oxygen content in the brain. The microelectrode comprises: a carbon fiber electrode; a hydrophobic modification layer, wherein the hydrophobic modification layer is formed on an outer surface of the carbon fiber electrode; and a catalyst modification layer, wherein the catalyst modification layer is formed on the outer surface of the hydrophobic modification layer, and the catalyst modification layer is formed ISAs-Co / HNCs. The microelectrode provided by the invention caneffectively detect the oxygen content, especially realizes the in-situ detection of oxygen in the brain, realizes a direct four-electron oxygen reduction process, is not affected by the hydrogen peroxide reduction current at the same time, avoids the generation of excessive cytotoxic hydrogen peroxide, has a controllable and stable electrode interface, strong electrode detection stability and goodrepeatability, and has a wide application prospect.
Owner:INST OF CHEM CHINESE ACAD OF SCI

One-dimensional raster scanning measuring head

The invention provides a one-dimensional raster scanning measuring head. The one-dimensional raster scanning measuring head comprises a lower substrate, a measuring pin installation turning wheel, a tensioning turning wheel, a measuring pin, a flexible steel belt, a tensioning spring, a raster ruler and a raster ruler reading head; the measuring pin drives the measuring pin installation turning wheel to appropriately rotate, the flexible steel belt and the raster ruler on the flexible steel belt are accordingly driven to move relative to the raster ruler reading head, the position variable quantity of the measuring pin is accordingly converted into the reading variable quantity of the raster ruler, and the detection aim of the measuring head is achieved. According to the one-dimensional raster scanning measuring head, continuous and sensitive motions of the measuring pin are achieved through the traction capacity and the deformation recovery capacity of the tensioning spring; the one-dimensional raster scanning measuring head competes detection depending on the mechanical transmission principle and the raster optical principle rather than the self-inductance and mutual-inductance detection principle, the detection sensitivity is high, the anti-interference capacity is high, the structure is simple, the one-dimensional raster scanning measuring head is easy to implement, and the position variable quantity of the measuring head in a large scope can be measured.
Owner:XI AN JIAOTONG UNIV

Method for detecting pole piece folding and platform for detecting pole piece folding

The invention provides a method for detecting pole piece folding and a platform for detecting pole piece folding. According to the method for detecting pole piece folding, a pole piece is arranged between an X-ray source and a detection device, rays irradiated by the X-ray source on the pole piece and the periphery of the pole piece are projected on the detection device and converted into visible light through the detection device, and then a detection image of the pole piece is generated. According to the method for detecting pole piece folding, the X-ray source and the detection device are adopted, the pole piece is irradiated through the X-ray source, rays are converted into visible light through the detection device, the image is generated, different states can be displayed on the folding area on the pole piece and the generated image, and thus the folding condition of the pole piece can be detected and analyzed.
Owner:SVOLT ENERGY TECHNOLOGY CO LTD

Intelligent human-body acid-base detector and method for detecting parameters of physical and psychological health

The invention discloses an intelligent human-body acid-base detector and a method for detecting parameters of physical and psychological health. The detector comprises a pH sensor, a high-impedance amplifier and an adder. The detector is characterized in that the detector also comprises a MSP430F149 single chip microcomputer in which a basic detection program and a special detection program are written; the single chip microcomputer has a 12-bit A / D converter which can convert 0-2,500 mv analog signals about saliva acid-base state, which are acquired by the pH sensor, subjected to high-impedance operational amplification and processed by the adder, into digital signals; mv-A / D-PH conversion is completed by the single chip microcomputer; corresponding technical analysis is completed according to the special detection program; meanwhile, instructions are sent out to a FLASH speech chip and a display driver chip; and detection results are reported in characters and voice through an LCD and a loudspeaker. The invention is used for detecting and judging the pH value of human body fluid and the fatigue states, physical ability reserve and emotional stress of drivers.
Owner:徐加彬

Botnet detection system and method for IoT environment, and storage medium

The invention discloses a botnet detection system and method for an IoT environment, and belongs to the technical field of intrusion detection. The method comprises the following steps: a flow collector monitoring the flow of monitored IoT equipment, and obtaining a flow data packet conforming to a to-be-detected rule based on a white list mechanism; the storage unit storing a flow data packet acquired by the flow collector; the data packet analyzer analyzing the content in the traffic data packet stored in the storage unit and obtaining IP information after analysis, and the IP information comprising time, a source IP, a destination IP, a source address and a destination address; the feature extractor extracting all statistical features of the IP information and performing feature dimension reduction processing in feature engineering to obtain three statistical features ranked in the front; and the sample detector carrying out malicious behavior judgment detection on the obtained three statistical characteristics based on a detection model constructed by a decision tree algorithm. The botnet detection method and device are used for botnet detection of the IoT environment.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA

Fluorescent resonance probe and application and kit thereof

The invention provides a fluorescent resonance probe and an application and a kit thereof, and belongs to the field of biological chemistry. The fluorescent resonance probe is suitable for detection of a target nucleic acid segment and comprises a first fluorescence probe and a second fluorescence probe which can form a T-shaped structure together with the target nucleic acid segment. According to the T-shaped structure, fluorescent resonance energy transfer of a first fluorescence group on the first fluorescence probe and a second fluorescence group on the second fluorescence probe can be ensured. Qualitative or quantitative detection of the target nucleic acid segment is achieved through detecting the fluorescent resonance energy transfer efficiency. The fluorescent resonance probe has relatively high specificity and sensitivity, and the base sequence difference degree can be reduced to the target nucleic acid segment of one base for detection.
Owner:SHENZHEN INST OF ADVANCED TECH

Lesion data collection method, lesion identification method and computer device

The invention relates to a lesion data collection method, a lesion identification method and a computer device. The lesion data collection method includes methods that a lesion image sample of a to-be-detected part is obtained; according to a standard pathological report, the lesion position in the lesion image sample is found; according to lesion characteristics corresponding to the lesion position, the lesion part under an electronic endoscope is back-tracked. The lesion identification method is further disclosed, the lesion identification method comprises steps that lesion data is collected, the artificial intelligence deep learning network is utilized for training according to the lesion data to obtain a standard lesion identification model; the model is utilized to identify lesion data of a new to-be-detected part, and the lesion condition is determined. The computer device is further disclosed and includes a processor, a memory and a computer program which is stored on the memoryand operable on the processor. The lesion data collection method, the lesion identification method and the computer device are advantaged in that the lesion position can be accurately found through the electronic endoscope, and the probability of detecting the lesion in advance can be greatly improved.
Owner:BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV

Glass bottle defect detection device and image splitting device

The invention discloses a glass bottle defect detection apparatus and an image splitting device. The glass bottle defect detection apparatus comprises a plane light source, a polarizer, a diaphragm, the image splitting device, a lens and a camera, wherein the polarizer is arranged in front of the plane light source, the diaphragm is arranged in front of the polarizer, a to-be-detected glass bottle is located between the polarizer and the diaphragm, the image splitting device, the lens and the camera are coaxially arranged in front of the diaphragm and are increasingly far away from the diaphragm, the image splitting device comprises a Fresnel biprism, two obliquely intersecting incident planes of the Fresnel biprism face the diaphragm and are arranged to be corresponding to to-be-detected positions, an intersecting prism ridge of the two incident planes is perpendicular to a horizontal plane, a left or right half part of an emergent plane of the Fresnel biprism is plated with a polarizing film, and the polarization direction of the polarizing film is perpendicular to the polarization direction of the polarizer. According to the invention, the detection apparatus can realize detection of conventional and uneven stress defects of a glass bottle only by using one camera, is capable of measuring boundary dimension of the glass bottle and has the advantages of low detection cost, a small occupation space, high accuracy and a low false drop rate.
Owner:潍坊天恒检测科技有限公司

A one-dimensional raster scanning probe

The invention provides a one-dimensional raster scanning measuring head. The one-dimensional raster scanning measuring head comprises a lower substrate, a measuring pin installation turning wheel, a tensioning turning wheel, a measuring pin, a flexible steel belt, a tensioning spring, a raster ruler and a raster ruler reading head; the measuring pin drives the measuring pin installation turning wheel to appropriately rotate, the flexible steel belt and the raster ruler on the flexible steel belt are accordingly driven to move relative to the raster ruler reading head, the position variable quantity of the measuring pin is accordingly converted into the reading variable quantity of the raster ruler, and the detection aim of the measuring head is achieved. According to the one-dimensional raster scanning measuring head, continuous and sensitive motions of the measuring pin are achieved through the traction capacity and the deformation recovery capacity of the tensioning spring; the one-dimensional raster scanning measuring head competes detection depending on the mechanical transmission principle and the raster optical principle rather than the self-inductance and mutual-inductance detection principle, the detection sensitivity is high, the anti-interference capacity is high, the structure is simple, the one-dimensional raster scanning measuring head is easy to implement, and the position variable quantity of the measuring head in a large scope can be measured.
Owner:XI AN JIAOTONG UNIV

Microelectrodes and probes containing microelectrodes and their use for determining oxygen content in the brain

The present invention proposes a microelectrode and a probe containing the microelectrode and its application in measuring oxygen content in the brain, the microelectrode comprises: a carbon fiber electrode; a hydrophobic modification layer, the hydrophobic modification layer is formed on the carbon fiber electrode an outer surface; and a catalyst modification layer, the catalyst modification layer is formed on the outer surface of the hydrophobic modification layer, and the catalyst modification layer is formed by ISAs-Co / HNCS. The microelectrode of the present invention can effectively detect the oxygen content, especially the in-situ detection of oxygen in the brain, realizes the direct four-electron oxygen reduction process, and at the same time is not disturbed by the hydrogen peroxide reduction current, avoiding the generation of excessive cytotoxic hydrogen peroxide, With a controllable and stable electrode interface, the electrode detection has strong stability and good repeatability, and has wide application prospects.
Owner:INST OF CHEM CHINESE ACAD OF SCI

A system, method and storage medium for botnet detection in iot environment

The invention discloses a system and method for botnet detection in IoT environment, belonging to the technical field of intrusion detection. The present invention includes a flow collector monitoring the flow of the monitored IoT device, and obtaining flow data packets that conform to the rules to be detected based on a whitelist mechanism; a storage unit stores the flow data packets acquired by the flow collector; The content in the existing traffic data packets is parsed. After parsing, IP information is obtained. The IP information includes time, source IP, destination IP, source address and destination address; the feature extractor extracts all statistical features of the IP information and performs feature engineering. The feature dimensionality reduction processing is performed to obtain the top three statistical features; the sample detector based on the detection model constructed by the decision tree algorithm performs malicious behavior judgment and detection on the obtained three statistical features. The present invention is used for botnet detection in IoT environment.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA

A trityl-based luminescent free radical material and its preparation method and application

The invention provides a trityl-based light-emitting free radical material and a preparation method and application thereof, belonging to the technical field of organic light-emitting materials. In the trityl radical luminescent material provided by the present invention, the common feature is the trityl radical connected to the nitro group with a strong electron-withdrawing effect; the central trityl radical not only acts as a The luminescent construction group also serves as an activation group to make trityl radicals more likely to be nucleophilicly substituted by biothiols. The synergistic effect of the two units (trityl radical and nitro group) enables the material to realize the detection purpose of multiple responses to biological thiols; to realize the detection of cysteine ​​three channels (fluorescence, ultraviolet and EPR signals). According to the above strategies, the purpose of identifying biothiols in RGB patterning, simulated clinical assays and fluorescence imaging was achieved.
Owner:JILIN UNIV

A kind of fluorescence resonance probe and its application and kit

ActiveCN106754902BGuaranteed fluorescence resonance energy transferReduce distanceMicrobiological testing/measurementDNA/RNA fragmentationEnergy transferResonance
The invention provides a fluorescent resonance probe and an application and a kit thereof, and belongs to the field of biological chemistry. The fluorescent resonance probe is suitable for detection of a target nucleic acid segment and comprises a first fluorescence probe and a second fluorescence probe which can form a T-shaped structure together with the target nucleic acid segment. According to the T-shaped structure, fluorescent resonance energy transfer of a first fluorescence group on the first fluorescence probe and a second fluorescence group on the second fluorescence probe can be ensured. Qualitative or quantitative detection of the target nucleic acid segment is achieved through detecting the fluorescent resonance energy transfer efficiency. The fluorescent resonance probe has relatively high specificity and sensitivity, and the base sequence difference degree can be reduced to the target nucleic acid segment of one base for detection.
Owner:SHENZHEN INST OF ADVANCED TECH

Detection device for fluorescent probe

The invention discloses a detection device for a fluorescent probe, and relates to the technical field of fluorescent probes. The detection device comprises a detection assembly and a detection box, wherein the detection assembly is arranged in the detection box; a jacking structure is arranged in the detection box; and the jacking structure mainly comprises a connecting frame, a pair of sliding rails, a pair of sliding bodies, an external driving box, a pair of shaft bodies, a pair of wheel bodies, a traction rope and a motor. According to the detection device for the fluorescent probe, the fluorescent probe is detected on the detection assembly, a high-density diameter hole is formed in the detection assembly, and the purpose of detection is achieved through the goodness of fit of the diameter of the hole where the probe is placed.
Owner:TIANCHUANG ZHIKU CULTURE PROPAGATION SUZHOU CO LTD

TA2 pure titanium color metallographic corrosion method convenient to identify

The invention relates to a convenient-to-identify color metallographic corrosion method for TA2 pure titanium, which comprises the following steps: aiming at the metal structure characteristics of the TA2 pure titanium, selecting an ultrahigh-concentration ammonium bifluoride solution with the mass concentration of about 15-18% as a corrosive agent, and carrying out corrosion treatment on the metal surface in a wiping manner. The method is easy to operate and high in success rate, and the metallographic structure of the TA2 pure titanium can be clearly and accurately presented in a color metallographic mode. The method has the advantages of simple operation, good color contrast, no over-corrosion of the grain boundary and clear, visual and vivid grain combination characteristics, and can better realize the purpose of detecting the TA2 pure titanium color metallographic phase, thereby improving the efficiency and accuracy of metallographic phase detection.
Owner:725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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