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Solid electrochemical luminescence sensor for detecting mercury ions and preparation method and application of solid electrochemical luminescence sensor for detecting mercury ions

An electrochemical and mercury ion technology, applied in the field of heavy metal detection, can solve the problems of not being disclosed, and achieve the effect of stable signal, strong selectivity and good reproducibility

Active Publication Date: 2014-11-19
乐陵市金山电器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are no reports on the use of N-(4-aminobutyl)-N-ethylisoluminol (ABEI) to detect mercury ion content in water using solid-state electrochemiluminescence.

Method used

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  • Solid electrochemical luminescence sensor for detecting mercury ions and preparation method and application of solid electrochemical luminescence sensor for detecting mercury ions
  • Solid electrochemical luminescence sensor for detecting mercury ions and preparation method and application of solid electrochemical luminescence sensor for detecting mercury ions
  • Solid electrochemical luminescence sensor for detecting mercury ions and preparation method and application of solid electrochemical luminescence sensor for detecting mercury ions

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0042] A solid-state electrochemical luminescence sensor for detecting mercury ions. The sensor is surface-mounted electropolymerized ABEI, and then sequentially assembled with glutaraldehyde, DNA1, and T-Hg 2+ -T mismatch binding DNA2 and biotin glassy carbon electrode, the specific preparation steps are as follows:

[0043] (1) Preparation of glassy carbon electrode immobilized with DNA1

[0044] a. Glassy carbon electrodes with a diameter of 3-5 mm were polished with 1.0 μm, 0.3 μm, and 0.05 μm Al2O3 in sequence, then ultrasonically cleaned with ethanol and water in sequence, rinsed with water, and dried with nitrogen for later use;

[0045] b. Place the glassy carbon electrode obtained in step (a) in H containing N-(4-aminobutyl)-N-ethylisoluminol (ABEI). 2 SO 4 In the solution, carry out cyclic voltammetry scanning, the voltage range is 0.2 ~ 1.5 V, scan 20 times at a scanning speed of 0.05 V / s, so that ABEI is electropolymerized on the surface of the glassy carbon elec...

specific Embodiment 2

[0062] The method for detecting mercury ions using the solid-state electrochemiluminescence sensor prepared in the above specific embodiment two, the principle of detecting mercury ions is as follows figure 1 As shown, the specific steps are as follows:

[0063] a. After washing the glassy carbon electrode immobilized with DNA1 prepared in the above specific example 2 with 0.01-0.1 M PBS buffer solution, start the process in 0.05-0.2 M carbonate buffer solution with pH = 9-10 Electrochemical reaction, test the intensity of electrochemiluminescence I 0 ;

[0064] b. Put the solid-state electrochemiluminescence sensor for mercury ion detection prepared in the above specific example 2 into 0.05-0.2 M carbonate buffer solution with pH = 9-10, start the electrochemical reaction, and measure the intensity of electrochemiluminescence , to obtain a series of different concentrations of Hg 2+ The corresponding electrochemiluminescence intensity value I of the solution 1 , to calcul...

specific Embodiment 3

[0072] Highly selective and sensitive detection assay

[0073] High sensitivity is embodied by specific embodiment two, because the change value ΔI=I of electrochemiluminescence intensity 0 ?I 1 , has a linear relationship with the logarithm of the concentration of mercury ions in the concentration range of 0.01 to 10 nM. Therefore, the solid-state electrochemiluminescence sensor for detecting mercury ions prepared in the specific embodiment 1 is sensitive to Hg 2+ The detection sensitivity reaches 0.01 nM.

[0074] High selectivity: under the same experimental conditions as in the above-mentioned specific example 2, detect common interfering ions of 10 μM: Pb 2+ , Mn 2+ , Co 2+ , Ni 2+ , Cu 2+ , Zn 2+ , Cd 2+ , Mg 2+ , the result is as Figure 4 shown.

[0075] The results showed: 0.01 nM Hg 2+ The ΔI of the sensor is about 800, while the common interfering ions of 10 μM cause the ΔI of the sensor to be less than 200, which means that 1000000 times of common inter...

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Abstract

The invention discloses a solid electrochemical luminescence sensor for detecting mercury ions and a preparation method and application of the solid electrochemical luminescence sensor for detecting the mercury ions. The solid electrochemical luminescence sensor is characterized by being a glassy carbon electrode, wherein the surface of the glassy carbon electrode is immobilized with electrically polymerized ABEI, and then the glassy carbon electrode is assembled with glutaraldehyde, DNA1, T-Hg<2+>-T mismatched DNA2 and biotin. The preparation method comprises the steps: preparing the glassy carbon electrode immobilized with the DNA1 and assembling the solid electrochemical luminescence sensor. The solid electrochemical luminescence sensor has the advantages of high sensitivity, high stability, high selectivity, high repetitiveness, easiness in operation and the like, and reagents can be saved.

Description

technical field [0001] The invention relates to the technical field of heavy metal detection, in particular to a solid-state electrochemical luminescence sensor for detecting mercury ions, a preparation method and application thereof. Background technique [0002] Heavy metal pollution is one of the important components of environmental pollution. Mercury (Hg), the only liquid metal at room temperature, is the number one killer of heavy metal pollution. In recent years, the mining, smelting, processing and commercial manufacturing activities of heavy metal mercury have been increasing, and a large amount of mercury has entered the atmosphere, water and soil for retention, accumulation and migration. Mercury in the environment finally enters the body of animals and humans in the form of food chain, causing great damage to the vital functions of organisms, and causing diseases such as neuropsychiatric symptoms, tremors, stomatitis, and toxic nephropathy. Mercury ions (Hg 2+...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
Inventor 郭智勇陈贝贝沙玉红徐成银郝婷婷
Owner 乐陵市金山电器有限公司
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