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55results about How to "Avoid enzymatic digestion" patented technology

Composition for intestinal delivery

The present invention relates to a new composition, use and method for oral administration to a human or an animal of a physiologically active agent comprising neutralizing agents to increase pH in the digestive system to prevent denaturation, inhibitors of digestive enzymes to substantially prevent enzymatic digestion, and at least uptake increasing agents which increases intestinal absorption of a physiologically active agent, a drug and / or a nutrient.
Owner:PEROS SYST TECH

Method for extracting krill oil from antarctic krill

ActiveCN102492537AResidues in line with national standardsPrevent strong odorFatty-oils/fats productionOrganic solventLiquid ratio
The invention relates to a method for extracting krill oil from antarctic krill. The method comprises: heating the captured antarctic krill for 3-20 minutes at a temperature of 60-100 DEG C; drying the antarctic krill, wherein the drying temperature is 60-89 DEG C; crushing the dried antarctic krill into the krill powder with the particle size of 30-100 meshes; adopting an ethanol solution with the concentration more than 95% to soak the krill powder at the temperature of 4-30 DEG C, wherein a solid / liquid ratio is 1:2-1:10, and the time is 0.5-2 hours; carrying out shear extraction on the soaked suspension liquid by using a shearing emulsifying machine, and carrying out standing or stirring extraction on the resulting suspension liquid after the shear extraction; separating the extracting solution, and carrying out extracting separation on the extracting solution 2-4 times; and evaporating the resulting extracted solution at the temperature of 30-60 DEG C and under the vacuum degree of from -0.05 to -0.1 MPa to remove the organic solvent in the extracted solution to obtain the antarctic krill oil with the phospholipid content of 40-45%. According to the present invention, the operation of the method of the present invention is simple, the phospholipid content of the final product is high, and the method can be used as the most simple and feasible method for extracting the greases, and especially the phospholipid-rich greases.
Owner:辽渔南极磷虾科技发展有限公司

Preparation method of whitening complex protein peptide enzymatic hydrolysate, whitening complex protein peptide beverage and preparation method of whitening complex protein peptide beverage

The invention discloses a preparation method of whitening complex protein peptide enzymatic hydrolysate. The preparation method includes the following steps: (1) preparing raw materials; (2) preparingprotein enzymatic hydrolysate, and performing enzymolysis by using alkaline protease, papain and neutral protease through two steps, wherein the using amount of the alkaline protease is 1-3% of the dry weight of material liquid, the using amount of the papain is 0.5-1.5% of the dry weight of the material liquid and the using amount of the neutral protease is 1-3% of the dry weight of the materialliquid; (3) performing centrifugation; (4) performing decolorization; and (5) performing filtering so as to obtain the whitening complex protein peptide enzymatic hydrolysate. The full enzymolysis ofeach protein can be achieved through the preparation method, and active peptide can be reserved, so that the obtained complex protein peptide can be easily and rapidly absorbed, nutrition can be rapidly supplemented, and the complex protein peptide has the functions of promoting skin whitening. A whitening complex protein peptide beverage containing the whitening complex protein peptide enzymatichydrolysate and a preparation method of the whitening complex protein peptide beverage are also provided. The whitening complex protein peptide beverage has the effects of significantly promoting skin whitening and is tasty.
Owner:RUNKE BIOENG FUJIAN

Preparation method of compound protein peptide enzymatic hydrolysate capable of enhancing immunity, compound protein peptide beverage capable of enhancing immunity, and preparation method of compound protein peptide beverage

The invention discloses a preparation method of compound protein peptide enzymatic hydrolysate capable of enhancing immunity. The preparation method comprises the following steps of (1) preparing rawmaterials; (2) preparing plant protein enzymatic hydrolysate, wherein alkaline protease, flavourzyme and papain are used for enzymolysis in two steps; (3) preparing an animal protein enzymatic hydrolysate, wherein alkaline protease, trypsin and neutral protease are used for enzymolysis in two steps; (4) centrifuging; and (5) decolorizing and filtering to obtain the compound protein peptide enzymatic hydrolysate capable of enhancing immunity. The invention also provides a compound protein peptide beverage capable of enhancing immunity containing the compound protein peptide enzymatic hydrolysate, and a preparation method of the compound protein peptide beverage capable of enhancing immunity. Each protein can be fully subjected to enzymolysis, and the active peptide is reserved, so that theobtained compound protein peptide can be easily and quickly absorbed, and has the effects of quickly supplementing nutrition and improving immunity; the compound protein peptide beverage capable of enhancing immunity has a remarkable immunity-enhancing effect and is relatively delicious.
Owner:RUNKE BIOENG FUJIAN

Method for preparing cordyceps soy sauce

The invention discloses a method for preparing cordyceps soy sauce. The method comprises the following steps of: 1) mixing a cordyceps militaris culture residue and fermented extract oil in a mass ratio of 1:(5-10), boiling at the temperature of between 90 and 100 DEG C for 1 to 2 hours, soaking for 1 to 7 days, filtering, and extracting filtrate to obtain a cordyceps soy sauce stock solution; and 2) blending the cordyceps soy sauce stock solution, and sterilizing to obtain cordyceps soy sauce. According to the method, a starter propagation process of the cordyceps culture residue is eliminated, so that the problem of difficulty in starter propagation through ventilation due to high moisture content and high viscosity of the culture residue is solved; and the enzymolysis of nutrient substances in a culture medium can be prevented, so that active ingredients in the nutrient substances are better reserved. The cordyceps soy sauce prepared by the method is rich in multiple active ingredients such as cordycepin, cordycepose, cordyceps glycosides and the like which are beneficial to human bodies, rich in soy fragrance and more suitable for meeting the demands of consumers.
Owner:关卓愿

Method for constructing cDNA library

The invention discloses a method for building a cDNA library which is characterized in that the method is simple, quick and does not need endonuclease. The method essentially comprises the following steps: (1) a first cDNA chain is synthesized with general RNA (or mRNA) as the template through reverse transcription with the pair of primers. (2) a second cDNA chain is synthesized with the pair of primers through PCR catalyzed by Tap DAN synthetase. (3) the PCR product obtained in step (2) is directly connected with a T-carrier through DNA ligase, and then colon bacillus competence is converted to obtain the cDNA library. The method has very low cost in building the cDNA library, needs a very small number of RNA templates, and is applicable to biological sample RNA of any source.
Owner:HAINAN UNIVERSITY

Zolmitriptan tongue tablet

The invention provides a zolmitriptan sublingual tablet, which is characterized in that it contains the main drug zolmitriptan, a disintegrating agent and a filler. In every 1000 sublingual tablets, the content of zolmitriptan is 1 -50g, the content of disintegrating agent is 2-20g, the content of filler is 40-200g; it also contains 0-20g of corrective agent, 10-60g of binder and 0.5-5g of lubricant. The present invention utilizes the characteristics of non-keratinized sublingual mucosa, rich capillaries, and fast blood flow, aiming at the absorption of zolmitriptan ordinary tablets through the gastrointestinal tract, slow onset of action, first-pass effect, and low bioavailability. The deficiencies of advanced prior art, zolmitriptan is made into sublingual tablet, can make it absorb through sublingual mucosa, directly enter blood circulation through jugular vein and superior vena cava, take effect rapidly; Avoid oral administration The first-pass effect improves bioavailability and ensures curative effect. Moreover, water is not needed when taking the medicine, and it is placed under the tongue to contain it, so it is convenient to take and has a good taste. With the characteristics of rapid onset of action, convenient administration and high bioavailability, it can provide patients with a new treatment option and fill the gap in the market.
Owner:重庆医科大学医药研究所

Method for preparing Chinese medicinal composition for treating respiratory disease of livestock and application thereof

The invention provides a method for preparing Chinese medicinal composition for respiratory disease of livestock and application thereof and relates to extraction of a Chinese medicinal composition. The Chinese medicinal composition comprises indigowoad root, baical skullcap root, liquorice root, vietnamese sophora root, ephedra and platycodon root; the composition is prepared by the following steps: (1) obtaining an ethanol extract of the indigowoad root; slicing the scutellaria and soaking the sliced scutellaria in hot water; mixing the scutellaria with a hot leachate, the liquorice, the bushy sophora, the ephedra, the platycodon and indigowoad root medicinal residue obtained after the ethanol is extracted; slowly adding the ethanol into the obtained condensate with stirring till the alcohol content reaches 60 percent, reclaiming the ethanol at reduced pressure from a supernatant liquid till alcohol taste disappears to obtain a water extract, and making the water extract into oral liquid or particles in an amount of 100 kilograms, or 100,000 pills by tabletting, or injection by sterilization. The composition has the advantages of good treatment effect on preventing and curing the respiratory disease of livestock, wide sources of medicinal materials, low cost, simple preparation method, no medicament residue and safe clinical application.
Owner:LUOYANG HUIZHONG ANIMAL MEDICINE

Anti-tumor angiogenesis polypeptide mPEG-Mal-Cys-AS16

The invention belongs to the technical field of biological medicine, and particularly relates to anti-tumor angiogenesis polypeptide mPEG-Mal-Cys-AS16. The peptide comprises 17 amino acids, and the specific amino acid sequence is mPEG-Mal-CATWLPPRAANLLMAAS. AS16 is subjected to proper chemical modification through adopting polyethylene glycol, so that the purpose that the half-life period of the modified AS16 in an organism is prolonged is achieved. Experiments prove that compared with the original polypeptide AS16, the polypeptide mPEG-Mal-Cys-AS16 subjected to chemical modification provided by the invention has the advantages that the biological activity is well maintained, the polypeptide is prevented from being subjected to enzymolysis of a plurality of enzymes in the organism or being filtered by the glomerulus, and the half-life period of the polypeptide is prolonged well, so that better application value is achieved when the polypeptide is applied to tumor inhibition.
Owner:ZHENGZHOU UNIV

Preparation method of Maca active extracts

The invention provides a preparation method of Maca active extracts. According to the method, firstly, fresh Maca roots or dry Maca roots are pre-treated, then, percolation is carried out for twice after the treatment by a pre-heated mixed solvent, freeze drying is carried out after additives are added into concentrated liquid, and the Maca active extracts are obtained. The method provided by the invention is simple, the operation is easy, the nutrition ingredients and active substances of the Maca are sufficiently and fully integrated, the active ingredient enzymolysis is prevented, and in addition, the loss and the damage of nutrition ingredients and active ingredients can be effectively prevented. The obtained product can realize the instant eating effect, can also be used as raw materials to be made into health care foods in tablets, capsules and the like, or can be added into various kinds of food as additives to realize the application.
Owner:KUNMING UNIV OF SCI & TECH

Composition for modulating a physiological reaction or inducing an immune response

The present invention relates to a new composition, uses and method for modulating a physiological reaction or inducing an immune response after oral administration to a human or an animal of a physiologically active agent comprising (at least one) neutralizing agents to increase pH in the digestive system to prevent denaturation, (one) inhibitors of digestive enzymes to substantially prevent enzymatic digestion, and at least (one) uptake-increasing agents which increases intestinal absorption of a physiologically active agent, a drug and / or a nutrient.
Owner:PEROS SYST TECH

Hybrid peptide expressed via Bacillus subtilis, and preparation method and application thereof

The objective of the invention is to provide a hybrid peptide expressed via Bacillus subtilis, and a preparation method and application thereof. The sequence of the hybrid peptide is as shown in SEQ ID No. 1 in a sequence table. The preparation method comprises the following steps: artificially designing a fusion gene of SP<AmyQ>-SUMO-PR-FO and SP<SacB>-SUMO-PR-FO; inserting EcoRI and BamHI enzyme sites at two terminals; linking a target gene with an expression vector pGJ148; transferring a plasmid into the competent cell of Bacillus subtilis by using a chemical approach and inducing fermentation via maltose so as to produce a fusion expression product; and carrying SUMO digestion so as to obtain the hybrid peptide PR-FO. The antibacterial peptide PR-FO is obtained by subjecting PRW4 and alpha-helical antibacterial peptide fowlicidin-2 to heterozygosis and presents strong bactericidal activity and high stability and cell selectivity compared with the original peptide PRW4.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Antibody preservation solution and preparation method thereof

ActiveCN106800598AHigh activityPrevent proteolysisPeptide preparation methodsImmunoglobulinsPro-Gly-ProChemistry
The invention discloses an antibody preservation solution. The antibody preservation solution is prepared from a buffer solution, 4-6% of trehalose, 0.005-0.01% of sodium azide, 30-50% of glycerol, 0.002-0.0025% of first polypeptide and 0.002-0.0025% of second polypeptide, wherein the buffer solution is 40-100mmol / L Tris-HCl buffer solution with a pH value of 7.0-7.5; the concentrations of the trehalose, the sodium azide, the glycerol, the first polypeptide and the second polypeptide are respectively the mass ratio; the amino acid sequence of the first polypeptide is Val-Pro-Thr-Gly-Ala-Pro; the amino acid sequence of the second polypeptide is Ile-Pro-Gly-Pro-Val-Gly. The invention also discloses a preparation method of the antibody preservation solution. The antibody preservation solution provided by the invention can be stored at room temperature for a long time, and can enable an antibody to maintain higher activity.
Owner:广州桂雨生物科技有限公司

Processing method for particle-type small leaf Kuding tea

The invention discloses a processing method for particle-type small leaf Kuding tea, and belongs to the technical field of tea processing. The processing method includes the following steps that fresh leaf picking, fresh leaf tedding, thermal rinsing, dehydrating, hammer cutting, rolling cutting, primary drying, stirring frying shaping, enough drying and fragrance raising are carried, leaves of small leaf Kuding tea growing in karst landform and at the altitude of 600 m or above are selected as fresh tea leaves, edible salt and camellia are added during thermal rinsing, dehydration is carried out after green scalding, hammer cutting and rolling cutting are carried out, broken tea is obtained, stirring frying shaping is carried out after primary drying, tea obtained after primary drying is put into a twisting machine at the temperature of 60 DEG C to 80 DEG C, stirred, fried and shaped for 30 min to 50 min, and tea is crimped into particle-type bases; the fried particle-type bases are put into a frying drying machine and rolled for 1 h to 1.5 h at the temperature of 80 DEG C, the appearance is oleosus and smooth, stable particle shapes are formed, enough drying and fragrance raising are carried out, and the particle-type small leaf Kuding tea is obtained. The particle-type small leaf Kuding tea processed with the method is high in integrity, the amount of smashed tea is small, the appearance and the color are raven and oleosus, particles are round and compact, the fragrance is high, the taste is rich and long, and the flowery flavor is brought.
Owner:YUQING GOUPITAN TEA IND CO LTD

System for preparing sizing agent

The invention discloses a system for preparing a sizing agent. The system comprises a supplying device, a grading device, a first conveying device, a sand sediment trap, a second conveying device, an impurity removing device, a smashing device, a third conveying device and a pulping device, wherein the impurity removing device is arranged in a conveying line of the second conveying device; the third conveying device is connected with the smashing device and the pulping device; the pulping device is connected with sand sediment trap; the sand sediment trap comprises a trap body, an inlet and an outlet; a small-particle-size raw material or slurry formed by the small-particle-size raw material and water enters the trap body through the inlet; the trap body comprises a side wall and a bottom; the outlet is formed on the side wall; and the bottom is provided with at least one bump and / or at least one recess. According to the system for preparing the sizing agent, fine impurities are cleared by using the sand sediment trap; and light impurities are cleared by using the impurity removing device, so that clearing of the light impurities is realized.
Owner:COFCO GROUP +1

Soybean oligopeptide powder and preparation method thereof

The invention relates to soybean oligopeptide powder. The soybean oligopeptide powder comprises the following raw materials in parts by mass: 88-95% of soybean oligopeptides, 4-10% of stachyose, 0.7-1.4% of glycine, and 0.3-0.6% of aspartame. A preparation method of the soybean oligopeptide powder comprises the following steps: step 1, dispersing and hydrolyzing soybean protein isolate powder, namely blending the soybean protein isolate powder with deionized water so as to obtain protein pulp, and carrying out shearing so as to have the protein pulp dispersed and hydrated; step 2, carrying outultra-filtration while performing enzymatic hydrolysis, namely regulating the pH value of the hydrolyzed protein pulp to 8-9, adding compound protease of which the mass is equivalent to 1-4% of the mass of the soybean protein isolate powder, and carrying out enzymatic hydrolysis by performing agitation at 50-55 DEG C for 3-4 h, wherein the whole enzymatic hydrolysis process is carried in an agitation-ultrafiltration device; and step 3, obtaining a filtrate subjected to the agitation-ultrafiltration after completion of enzymatic hydrolysis, adding the stachyose, the glycine and the aspartame into the filtrate, carrying out uniform stirring, and carrying out concentrating, sterilizing and spray-drying so as to obtain the soybean oligopeptide powder. The soybean oligopeptide powder preparedby the preparation method has higher physiological activity.
Owner:江苏天美健大自然生物工程有限公司

Wool fabric anti-felting finishing method with high-strength retention rate

The invention relates to the technical field of fabric modification, and discloses a wool fabric anti-felting finishing method with high-strength retention rate. The method comprises the following steps of 1) putting the wool fabric into a hydrogen peroxide treatment liquid for oxidation treatment to obtain a wool fabric a; 2) adding the wool fabric a into an epoxy propyl dodecyl diethyl ammoniumchloride solution for surface modification to obtain the wool fabric b; 3) preparing composite protease microspheres; and 4) enabling the wool fabric b to be soaked into a finishing liquid comprisingthe composite protease microspheres for soaking finishing. The finishing liquid comprising the composite protease microspheres is adopted for finishing the wool fabric, strong damage to the wool fibers caused by the fact that the protease falls into the wool fibers from the space between scale layers on the surface of the wool fibers is avoided, and the wool fiber obtained through finishing has excellent anti-felting performance and high strength retention rate, thereby obviously improving the wearing property of the wool fabric.
Owner:胡明华

Sea cucumber composite element fine powder capsule and preparation method thereof

The invention relates to the technical field of sea cucumber products, and in particular, relates to a sea cucumber composite element fine powder capsule and a preparation method thereof. The capsulepowder includes the raw materials of dried sea cucumber, pueraria root powder, herba epimedii, rhizoma polygonati powder, rhizoma alismatis, apricot kernel, carotene powder, dendrobium nobile, purslane, hydroxypropyl starch and a flavor endowing agent. Provided are the sea cucumber composite element fine powder capsule and the preparation method thereof. The capsule powder has wide sources of rawmaterials and reasonable formula. Through the synergistic cooperation of various traditional Chinese medicines and sea cucumber powder ingredients, the capsule can effectively play roles in nourishingqi and blood, alleviating fatigue and improving human resistance. Capsule powder granules subjected to secondary refinement treatment are more easily digested and absorbed by a human body, and make the capsule have better and more effective health-care effect. The taste of the capsule powder is improved by the flavor endowing agent composed of xylitol, ethyl maltol and citric acid.
Owner:福建冠丰生物科技有限公司

Microscopic observation method of plant tissues

The invention discloses a microscopic observation method of plant tissues. According to the microscopic observation method of the plant tissues, disclosed by the invention, after fixing treatment is carried out, potassium hydroxide is used for digesting; transparent treatment is carried out; enzymolysis or other operations are avoided so that the cost is saved; the transparent effect is good and the compatibility with fluorescent staining and marking operation is good: an inner structure of plant deep-layer tissues can be clearly observed through the fluorescent staining under a fluorescence microscope. The microscopic observation method of the plant deep-layer tissues, which combines digestion treatment and a transparent technology, lays a foundation for phenotypic identification of advanced plants and deep researches on a gene expression mode, especially plays an important role in researches of observing the structure of the plant deep-layer tissues and has a high actual application value.
Owner:INST OF BOTANY CHINESE ACAD OF SCI

Maca coffee producing device and method

InactiveCN103976112APrevent enzymatic degradationProduct particles are uniformCoffee extractionWater circulationWater storage tank
The invention discloses a Maca coffee producing device and method. The Maca coffee producing device comprises a water storage tank, a booster pump, a preheater, an extraction tank, a cooling tank, an extraction liquid receiving tank, a separation and drying device and a product gathering tank, which are sequentially connected through pipelines, wherein a feed hole and a discharge hole of the extraction tank are communicated with each other through a circulation pump. The Maca coffee producing method comprises the followings steps: firstly carrying out overheating water circulation extraction on ground Maca coffee, baked coffee bean flour and vanilla planifolia bean flour, then cooling, carrying out centrifugal filtration, adding auxiliary materials, stirring for dissolving, and finally carrying out microfiltration and spray drying, thus obtaining a product. The Maca coffee producing device and method have the advantages that mixed extraction is adopted, no organic solvent is used, the process is simple and safe, the product has good effects, and the continuous large-scale production is facilitated.
Owner:LIJIANG BAISUIFANG BIOTECH

Method for extracting krill oil from antarctic krill

ActiveCN102492537BResidues in line with national standardsPrevent strong odorFatty-oils/fats productionOrganic solventLiquid ratio
The invention relates to a method for extracting krill oil from antarctic krill. The method comprises: heating the captured antarctic krill for 3-20 minutes at a temperature of 60-100 DEG C; drying the antarctic krill, wherein the drying temperature is 60-89 DEG C; crushing the dried antarctic krill into the krill powder with the particle size of 30-100 meshes; adopting an ethanol solution with the concentration more than 95% to soak the krill powder at the temperature of 4-30 DEG C, wherein a solid / liquid ratio is 1:2-1:10, and the time is 0.5-2 hours; carrying out shear extraction on the soaked suspension liquid by using a shearing emulsifying machine, and carrying out standing or stirring extraction on the resulting suspension liquid after the shear extraction; separating the extracting solution, and carrying out extracting separation on the extracting solution 2-4 times; and evaporating the resulting extracted solution at the temperature of 30-60 DEG C and under the vacuum degree of from -0.05 to -0.1 MPa to remove the organic solvent in the extracted solution to obtain the antarctic krill oil with the phospholipid content of 40-45%. According to the present invention, the operation of the method of the present invention is simple, the phospholipid content of the final product is high, and the method can be used as the most simple and feasible method for extracting the greases, and especially the phospholipid-rich greases.
Owner:辽渔南极磷虾科技发展有限公司

System for preparing ethanol

The invention provides a system for preparing ethanol. The ethanol preparation system comprises a potato crushing unit, a slurry preparation device and a fermentation unit. The slurry preparation device utilizes crushed products provided by the potato crushing unit to prepare slurry, and the slurry is provided for the fermentation unit. The potato crushing unit comprises a crushing device and a sedimentation tank. The crushing device and the sedimentation tank are connected to the slurry preparation device for preparing slurry. The fermentation unit comprises a communicating device communicating with an interface of a flash tower. According to the fermentation unit provided by the invention, with the potato crushing unit, continuous sand removing and impurity removing can be carried out when the potatoes are crushed. With the fermentation unit, a pipeline obstruction problem is avoided, and enzymolysis efficiency and fermentation efficiency are substantially improved. Therefore, continuous production of ethanol can be ensured.
Owner:COFCO GROUP +1

Capillary Electrophoresis Method for Fine Structural Analysis of Enoxaparin Sodium

A capillary electrophoresis method for quantitatively analyzing characteristic oligosaccharide present in enoxaparin sodium is provided in this invention. The method may be used for quantitatively determining the contents of disaccharides, trisaccharides, tetrasaccharides and in particular oligosaccharides having a 1,6-anhydro ring, which are unique compounds for enoxaparin sodium, within an exhaustively digested enoxaparin sodium sample with a mixture of heparinase I, II, and III, so as to quantitatively determine the molar percentage of oligosaccharides having 1,6-anhydro ring in enoxaparin sodium. The method may be used for the pharmaceutical quality control of enoxaparin sodium during the manufacturing process.
Owner:HANGZHOU JIUYUAN GENE ENG +1

Method for simultaneously extracting polysaccharide and alkaloid in dendrobium officinale

The invention discloses a method for simultaneously extracting polysaccharide and alkaloid in dendrobium officinale. The method comprises six steps of pretreatment, surfactant infiltration, steam explosion, microwave treatment, flocculation, membrane filtration separation and the like. According to the invention, the combined extraction method mainly adopting a surfactant and steam explosion is creatively adopted, and microwave, flocculation, membrane separation and other processes are combined, so that polysaccharide and alkaloid in dendrobium officinale can be fully extracted at the same time, the extraction rate is high, the defects of solvent residue, material structure damage and the like caused by extraction, enzymolysis and other treatments of a large amount of organic solvents are overcome, and the effect is obvious. The extraction rate of alkaloid exceeds 0.03%, the extraction rate of polysaccharide exceeds 27%, the purity of alkaloid and polysaccharide is high, and the application prospect is wide.
Owner:大冶康之堂农业发展有限公司
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