Antibody preservation solution and preparation method thereof

Abstract

The invention discloses an antibody preservation solution. The antibody preservation solution is prepared from a buffer solution, 4-6% of trehalose, 0.005-0.01% of sodium azide, 30-50% of glycerol, 0.002-0.0025% of first polypeptide and 0.002-0.0025% of second polypeptide, wherein the buffer solution is 40-100mmol / L Tris-HCl buffer solution with a pH value of 7.0-7.5; the concentrations of the trehalose, the sodium azide, the glycerol, the first polypeptide and the second polypeptide are respectively the mass ratio; the amino acid sequence of the first polypeptide is Val-Pro-Thr-Gly-Ala-Pro; the amino acid sequence of the second polypeptide is Ile-Pro-Gly-Pro-Val-Gly. The invention also discloses a preparation method of the antibody preservation solution. The antibody preservation solution provided by the invention can be stored at room temperature for a long time, and can enable an antibody to maintain higher activity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antibody preservation solution and a preparation method thereof. Background technique [0002] Antibody refers to the immunoglobulin produced by plasma cells differentiated from B lymphocytes by the body's immune system under the stimulation of antigens, and can specifically bind to corresponding antigens. Because antibodies can specifically and stably bind specific antigens, they are widely used. In the process of antibody utilization, whether it is properly preserved or not directly affects the activity and use effect of the antibody. For most antibodies, it is usually stored at low temperature. Using this method of storage, repeated freezing and thawing of the antibody will lead to denaturation of the antibody, multiple Aggregate production, thereby reducing antibody activity. [0003] How to provide an antibody preservation solution that can be stored for a long time at room...

AI Technical Summary

Problems solved by technology

In the process of antibody utilization, whether it is properly preserved or not directly affects the activity and use effect of the antibody. For most antibodies, it is usually stored at low temperature. Using this method of storage, repeated freezing and thawing of the antibody will lead to denaturation of the antibody, multiple Polymerization, thereby reducing antibody activity