The invention discloses a trivalent recombinant
adenovirus vaccine for foot-and-mouth
disease and a construction method of the trivalent recombinant
adenovirus vaccine. The trivalent recombinant
adenovirus vaccine is obtained by constructing a recombinant adenovirus vector co-expressed by three
antigen genes of a foot-and-mouth
disease virus and packaging the recombinant adenovirus vector by AY293-6015 cells, and the trivalent recombinant adenovirus vaccine can be used for simultaneously preventing three
serotype foot-and-mouth diseases. E1, E3 and E4 genes of an adenovirus vector are knocked out through
CRISPR, and shuttle plasmids in E1 and E4 regions are constructed and are separately used for expressing A-P12A, OBY-P12A and OXJ-P12A-3C-
mutant genes; and three foot-and-mouth
disease virus structural proteins share one 3C
protease to form a VLP, compared with a first-generation adenovirus vector, the vector capacity is increased by about 3kb, a recombinant adenovirus with
high titer can be obtained, and the vaccine capacity can be greatly improved when the recombinant adenovirus vector is used for preparing a recombinant adenovirus vaccine for the foot-and-mouth disease. Due to a mode of simultaneously expressing independent-complete-structure
protein antigens of three foot-and-mouth disease epidemic strains on one adenovirus vector, foot-and-mouth disease
virus A type, O type BY strains and O type XJ strains can be simultaneously prevented, and the specific immune response to the foot-and-mouth disease virus can be enhanced.