The invention discloses a preparation method of Bi-2212 high-temperature superconductivity wire, comprising the following steps: firstly, filling superconductivity powder into a pipe; secondly, drawing to process and prepare a secondary compound; thirdly, after drawing the secondary compound, combining the secondary compound with a pure silver rod and a silver alloy rod, and then packing the compound into an AgMn alloy pipe so as to obtain a third compound; fourthly, drawing and processing the third compound into a wire; fifthly, carrying out semi-fused treatment on the wire, and obtaining the Bi-2212 high-temperature superconductivity wire after cooling a furnace. As for the preparation method, the process is simple, the design is reasonable, the manufacturing cost is low, and the application range is wide, and the method can be effectively applied to the preparation process of other relevant superconductivity wires. In the invention, one silver or silver alloy core wire is additionally arranged at the central part of a multi-core wire; therefore, the core wire can be prevented from being broken at the central part of multi-core Bi-2212 wire, the core breakage rate of the wire is obviously reduced, the processing uniformity of the wire is improved, the final uniformity of the wire is improved, and simultaneously the stretching performance of the wire is also improved; and the preparation method is beneficial to the winding of magnet and other applications of superconductivity wire.