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5797 results about "Subtilis bacillus" patented technology
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Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants and humans. A member of the genus Bacillus, B. subtilis is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions.
Treatment of irritable bowel syndrome using probiotic composition
Owner:COBB & ASSOCS
Site-specific serine recombinases and methods of their use
InactiveUS20080020465A1Antibacterial agentsHydrolasesStreptococcus pyogenesSite-specific recombination
The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis phage recombinase, provided that when the first recombination attachment site is attB, the second recombination attachment site is attP and when the first recombination attachment site is attP, the second recombination attachment site is attB. The invention also describes compositions, vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors and methods of the present invention are also useful in gene therapy applications.
Owner:PADIDAM MALLA
Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof
ActiveCN101401921ALow costImmunity exceedsFungiAnthropod material medical ingredientsHouttuyniaLactobacillus acidophilus
The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.
Owner:河南省龙腾高科实业有限公司
Ferment for tobacco fermentation and use thereof
ActiveCN101416778AIncrease aromaReduce mistTobacco preparationFungiNicotiana tabacumBacillus aryabhattai
The invention discloses a hametz used for fermenting tobacco and applications thereof. The hametz is characterized by comprising three strains of Bacillus subtilis, lactobacillus and microzyme, the viable count ratio of which is 10 to 50: 2 to 30: 2 to 30. The hametz can ferment waste material of the tobacco or the flavor used for producing natural cigarettes by using the tobacco and can be used for tobacco fermentation. The flavor used for the natural cigarettes prepared by the hametz has a velvet fragrance and can remarkably increase the fragrance, reduce the unpurified gas and reduce the thrill as well when the flavor is used in the cigarettes, thus leading the smoke gas to be lingering and smooth. The hametz used for fermenting tobacco leaves can remarkably shorten the alcoholizing time and improve the quality of the tobacco leaves.
Owner:SOUTH CHINA UNIV OF TECH
Aquifer amendment containing multiple active microorganisms and method of preparing the same
The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.
Owner:SHANGHAI SIJI BIOLOGICAL TECH
Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof
The invention relates to a composite microbial fertilizer for controlling crop diseases and insect pests, which contains composite microbial fungicide and is prepared by the following raw materials based on parts by weight: 10-35 parts of composite microbial fertilizer fungicide, 3-15 parts of composite microbial pesticide fungicide, 1-5 parts of insecticidal synergist, 1-5 parts of plant growth regulator, 20-60 parts of grass carbon, 5-15 parts of expanded chicken manure, 5-50 parts of inorganic fertilizer and 1-3 parts of binding agent, wherein the composite microbial fertilizer fungicide is prepared by bacillus megaterium and jelly-like bacillus; and the composite microbial pesticide fungicide is prepared by mixing bacillus thuringiensis and bacillus subtilis, adding sodium percarbonate type solid state oxygen-increasing agent for mixing and fermenting and finally absorbing by the grass carbon. The composite microbial fertilizer adopts strains with good suitability, and the strains are compounded according to a certain proportion to obtain the composite fungicide which has durable activity and stable performance; and the four beneficial strains are compatible with each other and performs the own functions, so that the multiple aims of resisting germs as well as diseases and insect pests, and increasing the crop yield can be achieved.
Owner:BEIJING SHUANGLONG AMMS TECH +1
Treatment of disease states and adverse physiological conditions utilizing anti-fungal compositions
InactiveUS20060177424A1Effectively ameliorateEffective regulationBiocideBacteria material medical ingredientsSinusitisVaginal Yeast Infections
A method for treatment or prophylaxis of a disease state or other physiological condition, e.g., autism, delayed development, acid reflux disease, vaginal yeast infections, impaired hearing, chronic ear infections, seasonal allergies, Fibromyalgia syndrome, Crohn's disease, colitis, irritable bowel syndrome, interstitial cystitis, acne, sinusitis, rheumatoid arthritis, chronic fatigue syndrome, asthma, attention deficit disorder, attention deficit / hyperactivity disorder, rosacea, multiple sclerosis, hyperglycemia, or Ménière's disease, by administration of an anti-fungal composition that includes at least one of the bacilli (1) Bacillus subtilis, (2) Lactobacillus sporogenes, and (3) Streptococcus faecalis.
Owner:COBB AND CO
Compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof
InactiveCN106434430AEliminate pollutionAchieve biodegradableFungiBacteriaEcological environmentBacillus megaterium
The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.
Owner:中山市润泽生物科技有限公司
Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
Owner:LEGEND WIN FINANCE
Micro-ecological preparation and application thereof
The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.
Owner:BEIJING DABEINONG TECH GRP CO LTD +1
Treatment of autism using probiotic composition
A method of treating autism using a probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders.
Owner:COBB & ASSOCS
Antimicrobial compounds from Bacillus subtilis for use against animal and human pathogens
ActiveUS7247299B2Enhanced inhibitory effectRetain viabilityAntibacterial agentsBiocideBacillus perfringensClostridium difficile (bacteria)
Antimicrobial compounds from Bacillus subtilis for use against animal and human pathogens. A novel strain of Bacillus subtilis was isolated from the gastrointestinal tract of poultry and was found to produce a factor or factors that have excellent inhibitory effects on Clostridium perfringens, Clostridium difficile, Campylobacter jejuni, Campylobacter coli, and Streptococcus pneumoniae. The factor(s) retain full viability and antimicrobial activity after heat treatment. The invention provides a method of treatment of pathogenic microorganisms including C. perfringens.
Owner:KEMIN IND INC
Anti-bacterial water-based paint and preparation method thereof
InactiveCN102702889AImprove aging resistanceImprove the pulverization performanceBiocideAntifouling/underwater paintsWater basedEscherichia coli
The invention relates to anti-bacterial water-based paint and a preparation method thereof. The paint comprises the following components in parts by weight: 0.2-11 parts of anti-bacterial agent, 8-33 parts of nano material, 23-64 parts of water-based resin dispersoid and 0.75-18 parts of adhesive resin or plasticizer. The preparation method comprises the following steps: firstly preparing a nano silver anti-bacterial agent; mixing deionized water, the anti-bacterial agent, a wetting agent, a dispersing agent and a defoaming agent and uniformly mixing, adding the nano material, uniformly dispersing to obtain the water-based dispersoid; adding the obtained water-based dispersoid to the mixed emulsion or water-based resin dispersoid, then adding the adhesive resin or plasticizer and various conventional assistants, stirring and dispersing evenly; adding pigments or colorant; and supplementing water to obtain the anti-bacterial water-based paint. The long-acting broad-spectrum antibacterial water-based paint has high fungicidal efficiency (more than 99%) on escherichia coli, staphylococcus aureus, black varietas of bacillus subtilis and the like and can reduce the high concentrate of organic matters of formaldehyde to the range of specified concentration index.
Owner:ANHUI JINDUN PAINT
Functional microbial fertilizer production by utilizing agricultural wastes
InactiveCN101665374ASuitable physical and chemical propertiesPromote fermentation and maturityFungiBio-organic fraction processingBacillus licheniformisBiotechnology
The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g / T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g / T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.
Owner:THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
Two-effects microbiological additives of forage specially used for ruminants
InactiveCN1559261AImproves rumen fermentationImprove payAnimal feeding stuffFood preparationBranFeed additive
A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.
Owner:INNER MONGOLIA SILONG BIOTECH
Method for preparing health-benefiting fermented soybean meal
ActiveCN103283961AIncrease nutritionHas antibacterial effectAnimal feeding stuffAntibiotic YHealth benefits
The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg / g, the reducing sugar reaches 20-80mg / g, the GABA reaches 0.317-0.5mg / g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu / g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU / g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.
Owner:ZHEJIANG UNIV OF TECH
Charcoal-based microbial soil conditioner and preparation method thereof
ActiveCN104789226AReduce hydrogen ion levelsRaise the pHAgriculture tools and machinesOther chemical processesAureobasidium sp.Bacillus thuringiensis
The invention discloses a charcoal-based microbial soil conditioner. The charcoal-based microbial soil conditioner comprises, by weight, 80-85 parts of charcoal, 10-13 parts of a composite microbial flora and 1-2 parts of an oxidized starch adhesive, and the composite microbial flora comprises photosynthetic bacteria, actinomyces, lactic acid bacteria, microzyme, Bacillus subtilis and Bacillus thuringiensis. The invention also discloses a preparation method of the charcoal-based microbial soil conditioner. The combined effect of charcoal and microbes alleviates soil acidification, reduces the unit weight of soil and increases the permeability and the water content of soil; soil organic matter formation is promoted to improve the validity of mineral elements; and the abundance of microbes at the rhizosphere of crops is cultivated to improve the rhizospheric environment of plants in order to make pathogen microbes difficultly grow and breed, so the insect diseases of crops is reduced, and soil heavy metal pollution is alleviated and restored.
Owner:迪斯科科技集团(宜昌)有限公司
Special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof
InactiveCN101775359AGood degradation activityEasy to storeFungiBacteriaBacillus licheniformisVaccination
The invention discloses a special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing one or a plurality of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaerieus, clostridium sporogenes, clostridium acetobutylicum, clostridium barati, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichodermareesei and aspergillus niger. Simultaneously, by taking the special microorganism composite bacterial agent as a vaccination bacterial agent, the invention provides an application method for decomposing the crops straws and accelerating the ferment to generate marsh gas; and the microorganism composite bacterial agent-treated crops straws have fast ferment speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no faeces of poultry, and have very wide application range.
Owner:刘相梅 +1
Preparation method and usage for novel lipopeptide type biosurfactant surfactin
InactiveCN101041846AStable fermentation processNo pollution in the processBiocideCosmetic preparationsFreeze-dryingSolvent
The invention discloses a preparing method of new type lipopeptide biosurfactant surfactin and appliance, which comprises the following steps: utilizing bacillus subtilis strain E8 (conserved in Chinese microbe conservative council common microbe center, the keeping number at 1107) to proceed microbe liquid ferment prepare surfactant surfactin; proceeding carbon source, nitrogen source and inorganic salt optimize for the needed liquid culture medium; getting fermentation liquor; centrifuging to remove thallus; acidifying supernatant; extracting rough extract; further-extracting through dissolvent; acidifying; collecting deposition; centrifuging; vacuum-drying; getting pre-refined product; freeze-drying through drainage pillar and FPLC extraction; getting product with high purity; making the output of acidifying extraction at 24-56 h reach 5-13g / L. The dominant fermentate molecular weight of high-yield strain E8 can reach 1022Da and CMC can reach 1 mu M, which is the lowest of reported surfactin CMC value.
Owner:INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
Fermented feed additive, preparation method and application
ActiveCN102550815ASlow down digestionPromote digestion and decompositionFood processingAnimal feeding stuffMonosodium glutamateBacillus licheniformis
The invention discloses a fermented feed additive, a preparation method and application thereof. The fermented feed additive is prepared by fermenting mixed strains and mixed auxiliary materials consisting of monosodium glutamate protein, bean pulp, bran, cottonseed meal, corn fibers, rice bran and powdered rice hulls, wherein the mixed strains are obtained by mixing activated strains of lactobacillus acidophilus, lactobacillus planetarium, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis in a proportion of (1-5): (1-5): (1-5): (1-5): (1-5): (1-5); and the dosage of the fermented feed additive is 5 to 100 percent of the weight of the animal feed when used for the animal feed. According to the fermented feed additive, the bean pulp, the bran, the cottonseed meal and other raw auxiliary materials are sufficiently utilized, so that the food consumption in the animal feeding process is reduced; the fermented feed additive can be used to partiallyor completely replace the animal feed, and antibiotic medicaments can be reduced or prevented from being used; and no medicament is left, so that the feeding cost of farmers is reduced.
Owner:WEIFANG ZHONGKEJIAYI BIO FODDER TECH
Solid microbe agent for degrading petroleum pollution, and petroleum products, and preparation method
ActiveCN101050435AEasy to makeLow costBacteriaContaminated soil reclamationMicroorganismMicrobial agent
This invention discloses a solid microbial agent for degrading petroleum pollutants and petroleum products. The solid microbial agent is prepared by: mixing primary seed solutions of Bacillus subtilis and Sphingobacterium multivorum at a volume ratio of (0.5-20):1, fermenting to obtain mixed bacterial fermentation solution, and mixing the mixed bacterial fermentation solution and turfy soil at a weight ratio of (0.1-1):1. The active living bacteria number of Bacillus subtilis is 106-1010 / mL, and that of Sphingobacterium multivorum is 107-1012 / mL. The solid microbial agent has such advantages as high activity in soil, rapid growth and no secondary pollution to environment, and can be used for biological bioremediation of petroleum polluted soil and petroleum product leakage fields.
Owner:山东百科利生态科技有限公司
Microecological preparation, preparation method, and applications thereof
ActiveCN103497906AImprove the body's immunity and disease resistancePlay a synergistic roleBacteriaBacteria material medical ingredientsLactobacillus acidophilusGrouper
The invention discloses a microecological preparation, a preparation method and applications thereof, and relates to the field of aqua culture. The microbiological preparation comprises at least one component selected from bacillus subtilis and bacillus pumillus, and at least one component selected from lactobacillus plantarum and lactobacillus acidophilus; wherein the bacillus subtilis comprises Bacillus subtilis 1A05680, the bacillus pumillus comprises Bacillus pumilus 1A08151, the lactobacillus plantarum comprises Lactobacillus plantarum 1A07806, and the lactobacillus acidophilus comprises Lactobacillus acidophilus PL7. The microecological preparation can be applied to preparation of preparations, which are used for promoting growth of prawn or grouper, improving immunity and disease resistance of prawn or grouper, preventing and treating diseases, increasing the survival rate, and purifying the culture water.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Microbial fertilizer producing by using agricultural wastes and preparation thereof
InactiveCN102515900AEasy to eliminateLight weightBio-organic fraction processingOrganic fertiliser preparationBiotechnologyBacillus licheniformis
The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g / T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W / W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g / T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.
Owner:斯华四
Compound probiotics preparation for biological organic fertilizers and preparation method thereof
InactiveCN102229900APromote growthImprove firmnessBio-organic fraction processingFungiAureobasidium sp.Streptomyces variabilis
The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.
Owner:周剑平 +1
Biological preparation capable of preventing and treating cruciferae club root and use thereof
InactiveCN101416641AGood control effectSolve prevention and control problemsBiocideBacteriaSucroseRussulaceae
The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.
Owner:YUNNAN AGRICULTURAL UNIVERSITY
Application of bsacillus subtilis, microbial inoculum thereof and preparations thereof to field of fruit freshness keeping
ActiveCN101870959AGood control effectBiocideFruit and vegetables preservationTreatment effectFermentation
The invention relates to bsacillus subtilis and microbial inoculum using the bsacillus subtilis as active ingredients, which are applied to the field of fruit freshness keeping. The bsacillus subtilis related by the invention is named as Bsacillus subtilis J18, which is preserved in China General Microbiological Culture Preservation and Management Center at present with the preservation number of CGMCC NO: 3665. Strains are prepared into fermentation agents after fermentation culture, and the fermentation agents are respectively mixed with calcium chloride and chitosan solution to be prepared into dry powder preparations and fruit glaze agents with obvious prevention and treatment effects on pear melasma after picking, black rot of pear, grape grey mould, watermelon wilt and the like. The bsacillus subtilis J18 belongs to a strain of antagonistic bacteria with wide-spectrum resistance on fruit and crop diseases.
Owner:GENLIDUO BIO TECH CO LTD
Bacillus subtilis capable of effectively degrading vomitoxin and application of bacillus subtilis
ActiveCN103243047AImprove degradation efficiencyStrong specificityBacteriaHydrolasesAdditive ingredientBacillus subtilis
The invention provides Bacillus subtilis ANSB471 capable of effectively degrading vomitoxin in feed and an application of the Bacillus subtilis. The invention also relates to a cultural method of the Bacillus subtilis strain and a method for applying fermentation liquor of the strain to degradation on vomitoxin in the feed. After reacting for 72 hours, degradation rate of the bacillus subtilis ANSB471 on the vomitoxin in the feed is 95%, and the degradation rate on the vomitoxin is still about 90% after subculture. The strain also can secrete amylase and is high in enzyme activity, and utilization rate of animals on starch in the feed can be increased. The Bacillus subtilis ANSB471 has high vomitoxin degradation activity, strong specificity and mild effect and can not damage nutritional ingredients in the feed.
Owner:河南亿万中元生物技术有限公司
Bacillus subtilis for efficient exogenous protein expression and high density culture
Belonging to the technical field of bioengineering, the invention discloses a bacillus subtilis for efficient exogenous protein expression and high density culture. According to the invention, a screening and separation method is employed to acquire a bacillus subtilis with high protein expression quantity, and the six genes srfC, spoIIAC, amyE, nprE, aprE and bamA are knocked out to obtain B. subtilis WS5 with a preservation number of CCTCC M 2016536. The bacillus subtilis WS5 is adopted as the expression host for construction of a beta-CGTase genetically engineered bacterium. The beta-CGTase genetically engineered bacterium is subjected to 3L fermentor culture, foams produced in the fermentation process are obviously decreased, a mirror does not detect spore production, almost no extracellular amylase and protease exist in the culture process, after 70h of fermentation culture, the beta-CGTase enzyme activity reaches 270U / ml, and DCW reaches 70g / L.
Owner:JIANGNAN UNIV
High-efficiency ferment for fermenting bean pulp and bean pulp fermentation technology using the ferment
The invention relates to a high-efficiency ferment for fermenting bean pulp, belonging to the biologic enzymolysis fermentation technical field. The high-efficiency ferment is formed by mixing bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus casei, saccharomyces cerevisiae and candida utilis. The fermentation technology comprises the following steps: crushing the bean pulp and corn; activating the strains; uniformly mixing the bean pulp and the fermentation liquor using a mixer; packing the mixed bean pulp in fermentation bag; fermenting the packaged bean pulp and processing fermented bean pulp: drying-crushing-detecting-packaging-finished product. The technology is simple and the product quality is stable and the content of the anti-nutrient factor in the bean pulp is eliminated and obviously reduced, therefore the bean pulp is a safe plant protein feeder for infant animal with balanced nutrient, good quality and low price and help for health growth of the infant animal, in replace of animal protein feeder of bone powder, fish powder or the like.
Owner:MINXIONG BIOTECH CO LTD LONGYAN FUJIAN
Controlling plant pathogens with bacterial/fungal antagonist combinations
InactiveUS20050096225A1Extended shelf lifeEffective controlBiocideClimate change adaptationPhytophthora sp.Trichoderma sp.
Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.
Owner:NOVOZYMES BIOAG AS
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