Application of bsacillus subtilis, microbial inoculum thereof and preparations thereof to field of fruit freshness keeping
A Bacillus subtilis, preservation technology, applied in the direction of fruit and vegetable preservation, application, fungicide, etc., can solve the problems of rot loss, fruit eating safety impact, economic loss, etc., and achieve significant control effect
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Embodiment 1
[0030] A Bacillus subtilis, which is characterized in that it is named Bacillus substilis J18, and is preserved in a preservation unit designated by the State Intellectual Property Office, the preservation name is China General Microorganism Culture Collection Management Center, and the preservation number is CGMCC No: 3665.
[0031] Production method of J18 microbial fermentation liquid
[0032] (1) Seed activation: Bacillus subtilis J18 strain was transferred from preserved glycerol bacteria into LB liquid medium, and cultured at 30° C., 150-250 r / min for 18 hours. Take the cultured bacterial suspension and dilute it, evenly spread it on the LB solid plate medium, cultivate it in a 25°C incubator for 48 hours, pick a single colony with a toothpick and inoculate it in the LB liquid medium test tube again, at 30°C, 150~250r / m Min culture overnight, transfer 5 mL of cultured bacterial suspension to a 500 mL Erlenmeyer flask filled with 150 mL of LB liquid medium and place in a ...
Embodiment 2
[0050] Production method of J18 microbial fermentation liquid
[0051] (1) Seed activation: Bacillus subtilis J18 strain was transferred from preserved glycerol bacteria into LB liquid medium, and cultured at 30° C., 150-250 r / min for 18 hours. Take the cultured bacterial suspension and dilute it, evenly spread it on the LB solid plate medium, cultivate it in a 25°C incubator for 48 hours, pick a single colony with a toothpick and inoculate it in the LB liquid medium test tube again, at 30°C, 150~250r / m Min culture overnight, transfer 5 mL of cultured bacterial suspension to a 500 mL Erlenmeyer flask filled with 150 mL of LB liquid medium and place in a shaker at 30°C for shaking culture at a speed of 150-250 r / min.
[0052] The LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, water 1000mL.
[0053] (2) Seed culture: Inoculate the activated bacterial suspension into LB liquid medium, culture at 150-250 r / min, 30° C. on a shaker for 16 hours.
[0054] (3...
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