56 results about "Recombinant Human Endostatin" patented technology

The invention was involved in that target protein expression strain and molecular partner were expressed in E.coli.at the same time at low temperature to prevent the formation of target protein inclusion body effectively and increase yields of soluble production largely. Soluble recombinant human endostatin and human angiostatin were increased largely by the technique. It provided a new simple and cheap technique to produce soluble recombinant protein with biological activity. It was applied in bioengineering pharmaceutical factory, gene engineering, biochemistry and molecular biology with high efficiency, cheap price, wide application and strong promotion.

The invention discloses a recombinant human endostatin liposome microbubble, which is prepared by a method in the following steps: dispersing DSPE-PEG (distearoyl phosphoethanolamine-polyethylene glycol) 2000-Biotin and DSPC (disaturated phosphatidylcholine) in a molar ratio of 1:(0.5-4) in a phosphate buffer; introducing inert gas, mechanically oscillating, adding streptavidin, and incubating at room temperature, wherein the molar ratio of the streptavidin to the DSPE-PEG2000-Biotin is 1:(1-2); and adding biotinylated recombinant human endostatin, and incubating at room temperature to prepare the recombinant human endostatin liposome microbubble, wherein the molar ratio of the streptavidin to the biotinylated recombinant human endostatin is 1:(1-2). The invention also discloses application of the recombinant human endostatin liposome microbubble in preparing a medicament for inhibiting tumor angiogenesis under ultrasound mediation. The recombinant human endostatin liposome microbubble has a tumor inhibition rate of 41.3 percent, which is remarkably superior to the inhibition effect of a single recombinant human endostatin liposome microbubble.

The invention discloses an induced expression method of recombinant human endostatin. The method comprises the following steps: fermenting engineering bacteria until the OD600 value of fermentation liquor is 10-15, adding an inducer isopropyl-beta-d-thiogalactoside (IPTG), a carbon source substance and a nitrogen source substance, so that the concentration of the IPTG in the fermentation liquor is 0.2-0.4mmol / L; cultivating at 30-37 DE C for 2.5-3.5 hours; adding the inducer IPTG, so that the concentration of the IPTG in the fermentation liquor is 0.1-0.3mmol / L, and continuing to cultivate for 3-5 hours. By adopting the induced expression method of the recombinant human endostatin disclosed y the invention, the requirements of large-scale production of the recombinant human endostatin can be met, and efficient induced expression is achieved at high cell concentration.

The invention relates to a method for preparing recombinant human endostatin (rh-endostatin) nanoparticles for injection. The nanoparticles comprise chitosan and the rh-endostatin. A coacervation process is adopted for preparing the nanoparticles, namely a coagulant is added into chitosan solution containing the rh-endostatin, the mixture is subjected to stirring, ultrasonic sound treatment and centrifugation, and finally the deposit is re-mixed and suspended in water to form the nanoparticles. The preparation method is simple, and the particle size of the obtained nanoparticles is 400 to 900nanometers.

The invention relates to a method to rebuilding human vivo skin chalone strain and the solubility expression that belongs to gene engineering field. The strain contains expression carrier pET-Endo that could be gained by inserting the human vivo skin chalone into carrier pET43.1a. For cultivating the strain, it is induced by using IPTG at the concentration of 0.1-1.0mmol / L and the temperature in 25-30 degree centigrade, and for 6-10 hours time period; the external albumen solubility expression quantity could reach over 80% of the total expression quantity. And the total expression quantity isabout 50% of the thallus total albumen quantity. Thus, the invention conquers the disadvantage of expressing the human vivo by inclusion body mode. It could be used to rebuild the human vivo skin chalone and the other solubility expression of rebuilding albumen.

The invention discloses a method for preparing a recombinant human endostatin sustained release microsphere. In the method, recombinant human endostatin is solved in buffer solution containing protein stabilizer to obtain an inner water phase; lacto-glycolic acid copolymer is solved in mixed organic solvent to obtain an inner oil phase; the mixed solution of the inner water phase and the inner oil phase is dispersed fast to obtain primary emulsion; the obtained primary emulsion is arranged in a stirring vessel which is filled with plant oil or mineral oil containing emulsifier to be stirred into water-in-oil type compound emulsion; the water-in-oil type compound emulsion is stirred continuously to volatilize the mixed organic solvent in the inner oil phase and then is filtered and separated by a microporous filtering film after the microsphere is solidified; the residual plant oil or mineral oil on the surface of the microsphere is washed by the solvent; and the microsphere finished product is obtained after vacuum drying. The microsphere prepared by the method has high drug loading quantity, high entrapment rate, proper burst release quantity and long sustained release period.

Disclosed is a recombinant human endostatin injection liquid, mainly consisting of a stabilizer, a buffer solution, and a recombinant human endostatin.