30 results about "Polymyxin E sulfate" patented technology

The invention relates to a method for separating and purifying polymyxin E methanesulfonic sodium from a mixture containing small-molecular weight impurities. The method adopts an ultrafiltration membrane with the cutoff molecular weight ranging from 1,000 to 10,000 to carry out separation and purification. The invention also relates to a method used for preparing high-purity E methanesulfonic sodium by controlling reaction conditions and making use of ultrafiltration membrane separation technology. The method comprises the following steps: (a) adding water to dissolve polymyxin E sulfate andthen adding formaldehyde solution in the solution to carry out reaction; (b) adding sodium bisulfite solution in the reaction products of step (a) to carry out reaction continuously; and (c) taking the reaction solution of step (b) and adopting the ultrafiltration membrane to carry out separation and purification. The method of separating and purifying the polymyxin E methanesulfonic sodium has the advantages of low pollution, low energy consumption, simple process, good repeatability and high purity and activity of obtained products.

The invention provides a culture medium for fermenting polymyxin B sulfate. Each 100ml of the culture medium is prepared from the following components: 6g to 12g of flour, 0.03g to 0.06g of high-temperature amylase, 0.4g to 2g of cottonseed meal, 0.3g to 1g of corn pulp, 0.01g to 0.05g of dipotassium phosphate, 0.2g to 0.6g of ammonium sulfate, 0.2g to 0.6g of calcium carbonate, 0.02ml to 0.08ml of a de-foaming agent and the balance of water. A method for fermenting and producing the polymyxin B sulfate comprises the following the steps: inoculating the culture medium with strain seed liquid for producing the polymyxin B sulfate; fermenting and culturing fermentation liquid at 26 DEG C to 32 DEG C, wherein the pH (Potential of Hydrogen) value is 6.7 to 7.4 at the beginning of fermenting; controlling the pH value of the fermentation liquid to be natural during a fermentation process, wherein the dissolved oxygen volume concentration of the fermentation liquid is 10 percent to 30 percent. All the components of the culture medium are cooperatively matched and the content of impurities B2P is controlled to be lower than 1.5 percent.

The invention discloses an external gel for skin wounds, which is made of the following raw materials in parts by weight: 1-3 parts of active component A, 4-6 parts of active component B, 10-10 parts of calendula extract 14 parts, 16-20 parts of Centella asiatica extract, 60-80 parts of auxiliary materials; the active component A is at least two of polymyxin B sulfate, neomycin sulfate and bacitracin; the active component Part B is at least one of lidocaine hydrochloride, pramoxine hydrochloride, procaine hydrochloride and menthol; the auxiliary materials include the following raw materials in parts by weight: 0.5-3 parts of carbomer, organic solvent 40‑75 parts, 20‑50 parts of purified water. The gel for external use of the present invention has a preventive effect on bacterial infection of skin wounds, and has analgesic and hemostatic effects on skin wounds; it can also promote the formation of wound capillaries and collagen fibers, reduce the days of wound healing, and can be used for burns, cuts, For the healing of skin wounds such as burns, the healed skin is smooth, without scars, and the healing effect is good.

The invention provides an anhydride crystal of polymyxin sulfate B1, B2 or mixture thereof as well as a preparation method of the crystal. The preparation method comprises the following steps: (1) adding water into the polymyxin sulfate B1, B2 or the mixture thereof to enable the solid to be completely dissolved to obtain a saturated solution; (2) adding an organic solvent into the saturated solution slowly and dropwise, or adding the saturated solution into the organic solvent slowly and dropwise, separating out the solid and controlling the temperature of the dropwise adding process to be within the range of 0 to 60 DEG C, wherein the organic solvent is selected from one or more of C1-C4 alcohol, C3-C4 ketone or ethyl acetate, and butyl acetate; (3) filtering out the solid and vacuum-drying to obtain the anhydride crystal of the polymyxin sulfate B1, B2 or the mixture thereof, wherein the polymyxin sulfate B1 has a crystal form 1 and a crystal form A. The anhydride crystal particlesof the polymyxin sulfate B1, B2 or the mixture thereof, prepared by the method, are loose, non-sticky and particularly favorable for industrialized production of medicine products. In addition, by themethod, impurities in the medicines can be effectively removed, higher purity and clarity are achieved, and the quality of the medicine products is greatly improved.

The invention discloses lactating sow feed which specifically comprises the following components in percentage by weight: 47 to 59 percent of corn, 15 to 21 percent of bean pulp, 1.5 to 2.3 percent of fish meal, 7.2 to 14 percent of bran, 8.5 to 12 percent of wheat middling, 1 to 2 percent of plant oil and 3.5 to 6 percent of a pre-mixed material, wherein the pre-mixed material consists of an additive and a pre-mixing agent; the additive comprises the following components in percentage by weight: 0.2 to 0.3 percent of a trace element, 0.03 to 0.04 percent of various vitamins, 0.05 to 0.1 percent of 10 percent of polymyxin E sulfate, 0.01 to 0.02 percent of complex enzyme, 0.01 to 0.02 percent of an acidifying agent, 0.05 to 0.1 percent of sodium bicarbonate, 0.1 to 0.25 percent of diaminocaproic acid, 0.05 to 0.1 percent of methionine, 0.2 to 0.3 percent of choline, 0.5 to 1.2 percent of codonopsis pilosula and 0.4 to 1.2 percent of astragalus mongholicus; and the pre-mixing agent comprises the following components in percentage by weight: 0.8 to 1.2 percent of mountain flour, 0.75 to 1 percent of calcium hydrophosphate and 0.25 to 0.35 percent of edible salt. The codonopsis pilosula and the astragalus mongholicus are added in the lactating sow feed to serve as traditional Chinese medicine additives, so that the lactating sow feed has the functions of supplementing qi, activating the blood, promoting the lactation and the like; the galactopoiesis of a lactating sow can be promoted; the feed utilization rate is improved; side effects are avoided; and the health of the lactating sow is guaranteed.

The invention discloses a blood purification material based on mussel biomimetic chemistry and a preparation method thereof. The blood purification material is composed of a porous adsorption base material, an adsorption layer and a coating film in sequence from the inside to the outside, and the adsorption layer is Polymyxa sulfate. One or more of B, polylysine hydrochloride, polyethyleneimine or polydopamine, and is wound on the porous adsorption substrate in the form of molecular chains; the coating film is polyethylene Butyral-gallic acid film or polyvinyl butyral film. The blood purification material is designed based on mussel biomimetic chemistry, and has the advantages of simplicity and convenience, mild conditions, environmental protection, and basically no influence on the material body, and the blood purification material is used in the field of blood purification medical devices.