The invention relates to a calorimetric method for detecting pesticides by using acetylcholin
esterase. According to the technical scheme, an enzymatic inhibition method and
cascade reaction are adopted; the acetylcholin
esterase is suppressed by pesticides, so that the enzymatic
reaction rate is reduced; the quantity of
choline products is reduced and is in direct proportion to the suppression degree; and furthermore, the amount of the
choline can be obtained by
cascade reaction heat of
choline oxidase and
catalase. By using the
cascade reaction, the intensity of an original thermal
signal as well as the detection sensitivity and the detection resolution are greatly improved, the
detection limit is reduced, and the detection accuracy is effectively improved; a
pesticide detection calorimetric method based on the acetylcholin
esterase is realized; the advantages of high sensitivity of the acetylcholin esterase to organophosphorus and
carbamic acid ester type pesticides and no interference caused by electrochemical active substances or colors and
turbidity of the sample on the calorimetric method are integrated; the in-site quick
pesticide residue detection is facilitated; and the large-scale application is facilitated.