59 results about "Choline oxidase" patented technology

The invention relates to a kit for rapidly detecting organophosphorus pesticide residues and an application method thereof. The kit comprises a C enzyme bottle, a T enzyme bottle, a C substrate reaction cup and a T substrate reaction cup, wherein the C enzyme bottle and the T enzyme bottle are sealed by using enzyme bottle sealing covers and are filled with an enzyme lyophilized three-enzyme mixing agent; the C substrate reaction cup and the T substrate reaction cup are provided with lyophilization and water-absorption cushions containing acetyl choline, luminol and dimethylbenzidine. The invention further relates to a method for applying the kit to rapidly detecting the organophosphorus pesticide residues. The kit takes acetylcholin esterase, choline oxidase and peroxidase as a catalysis system; a product obtained by hydrolyzing and oxidizing acetylcholine chloride and luminol have a chemiluminescence effect. A method for flexibly testing organophosphorus pesticides in agricultural products is established. The kit has the advantages of high detection speed, simple operation process, good stability and repeatability, no need of expensive instruments and the like, and is very suitable for carrying out field monitoring on the organophosphorus pesticides in farm markets.

The invention discloses a chemiluminescence sensing detection method for organophosphorus pesticide residues and application thereof. The method is characterized by comprising the following steps: by taking a chemiluminescent probe as an indicator, indicating the organophosphorus pesticides to inhibit the degree of reducing choline produced by acetylcholine chloride subjected to catalytic hydrolysis of acetylcholin esterase, coupling choline oxidase to catalyze choline to generate hydrogen peroxide, then oxidizing a luminescence probe to generate chemiluminiscence by virtue of hydrogen peroxide, carrying out a multi-step coupling reaction to realize sensing detection of the organophosphorus pesticide residues by utilizing a chemiluminescence apparatus. According to the sensing method, 96 samples can be detected within 20 minutes, and dichlorvos is taken as an example, the detection limit reaches 1nmol / L. The method is sensitive and rapid and can be used for blood sample monitoring of patients suffering from poisoning of foods, water samples and organophosphorus poisons.

A method for measuring sphingomyelin and phosphatidylcholine comprising incubating sphingomyelin and phosphatidylcholine with bacterial sphingomyelinase and bacterial phospholipase D, alkaline phosphatase, choline oxidase, peroxidase, N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline, and 4-aminoantipyrine, preferably for about 45 minutes. A blue dye is generated.

An optical fibre bio-sensor for distriminating the nature of peripheral nerve fibres is composed of optical fibre and sensing membrane which is fixed to the end of optical fibre by co-valent binding or physical adsorption. Said sensing membrane is prepared from high-molecular film containing oxygen indicator, choline oxidase and high-molecular membrane by one of three methods. Its advantages include no need of cutting nerve fibre, high speed and correctness and no poison and no wound to human body.

The invention relates to the fields of nanometer catalysis, environmental protection and analytical chemistry, and specifically includes a test strip for rapidly detecting pesticides. In-situ generation of iron ferric oxide nanoparticles with imitation peroxidase activity on test strips and modification of acetylcholinesterase and choline oxidase, when organophosphorus pesticides exist, it will irreversibly inhibit the activity of acetylcholinesterase , so that the amount of choline generated by the hydrolysis of acetylcholine is reduced, resulting in the reduction of hydrogen peroxide generated through the cascade reaction, and the color development of the chromogenic substrate becomes lighter. Moreover, the chromatographic phenomenon of the test paper can eliminate the interference of other substances in the sample. The convenient and quantitative detection of acetylcholine can be realized through mobile phone camera and mobile phone software analysis. The invention can be used in the fields of pesticide residue analysis, biomedicine, environmental monitoring and the like.

The invention provides an electrochemical detection method for fast identifying peripheral nerve bundle characteristic, which is an electrochemical detection method for qualitatively detecting acetylcholinesterase in peripheral nerve bundle which is based on detecting oxidative signal on electrode surface produced by enzyme reaction products. The method has the following steps: first using a polymer gold nanoparticle complex to modify electrode, which is then incubated in choline oxidase solution and acetylcholinesterase solution orderly, through good adsorption force of protein on complex film, the two kinds of enzymes enrich on the electrode surface, through three-electrode system, detecting the ampere response of substrate acetylcholine based on two enzymes system, then the detection of acetylcholinesterase is indirectly realized (detecting concentration range is: 1.0-2.0unit / ml). The invention is a breakthrough in the field of identifying clinical differential peripheral nerve bundle characteristic for incubating in nervous homogenates instead of standard acetylcholinesterase solution, according to whether acetylcholine has response; the invention realizes fast identification of motor tract and sensory tract.

The invention relates to a kit for rapidly detecting organophosphorus pesticide residues and a method for using the kit; the kit includes: C enzyme bottle, T enzyme bottle, C substrate reaction cup, T substrate reaction cup, the C enzyme bottle , T enzyme bottles are all sealed with enzyme bottle sealing caps, and are equipped with enzyme freeze-dried three-enzyme mixture; the C substrate reaction cup, T substrate reaction cup are equipped with acetylcholine, luminol and dimethyl Freeze-dried absorbent pads for benzidine. The present invention also relates to a method for rapidly detecting organophosphorus pesticide residues using the aforementioned kit. The invention uses acetylcholinesterase, choline oxidase and peroxidase as a catalytic system to hydrolyze and oxidize the acetylcholine chloride product with luminol to produce a chemiluminescent effect. Establish a method for sensitive testing of organophosphorus pesticides in agricultural products. The invention has the advantages of fast detection speed, simple operation process, good stability and repeatability, and does not require expensive instruments, and is very suitable for on-site monitoring of organophosphorus pesticides in farmers' markets.