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Test strip for rapidly detecting pesticides

A technology for test strips and pesticides, applied in the field of analytical chemistry, can solve rare problems, and achieve the effects of improving stability and simplifying preparation steps

Active Publication Date: 2019-01-08
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some urine sugar test strips, HIV test strips, and drug detection test strips have been reported, but there are few reports on test strips that generate nanozymes in situ and combine them with smartphones for pesticide detection

Method used

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  • Test strip for rapidly detecting pesticides
  • Test strip for rapidly detecting pesticides
  • Test strip for rapidly detecting pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Pesticide Test Strip

[0033] (1) Use a paper cutter to cut the filter membrane into a filter strip with a size of 0.2×2.5 cm;

[0034] (2) Use clamps to fix both ends of the filter membrane strip, and leave a 2 mm gap at 1-2 cm on the bottom edge of the filter membrane strip for subsequent preparation of test strips;

[0035] (3) Add 10% bovine serum albumin (BSA) solution, 1 mmol / L NHS, 0.5 mmol / L EDC equal volume mixed solution dropwise into the gap of the test strip (BSA is used to improve the nylon filter membrane Adsorption capacity and used as a template for the next reaction, EDC / NHS is to activate the carboxyl group on BSA), after incubating for 30 min at room temperature, washing and drying;

[0036] (4) Add 4 mmol / L FeCl 3 , 2 mmol / L FeCl 2 The same volume as 16 mmol / L ammonia water is uniformly dispersed in the gap of the filter membrane obtained in the previous step in order to generate in situ the ferroferric oxide mimic enzyme material (Fe 3 O 4 @B...

Embodiment 2

[0039] The detection area in the test strip has AchE, ChOx and peroxidase activity under weak acid conditions. AchE can catalyze Ach to produce choline, and ChOx can catalyze choline to H 2 O 2 , Imitation peroxidase catalyzes H 2 O 2 It reacts with organic color reagents. When the party pesticides are present, it will irreversibly inhibit the activity of AchE, leading to the H generated by the cascade reaction. 2 O 2 Decrease, the color of the organic developer becomes lighter, see the attached manual for the detection diagram figure 1 .

[0040] Test strip verification of the inhibitory effect of active pesticides:

[0041] Experimental system a: The catalytic reaction system is a sample solution containing Ach (0.1 mmol / L), the detection test strip fragments obtained in Example 1, the organic color developer TMB (0.5 mmol / L) and acetate buffer (pH 4.0, 100 mmol / L). After immersing the test strip in a buffer solution containing Ach and TMB for 5 min at room temperature (25°C), th...

Embodiment 3

[0045] Qualitative analysis of OP

[0046] Specific system: Prepare two test strips prepared earlier, one of which is immersed in a weakly acidic solution containing Ach (1mM) and TMB (0.5 mmol / L), and the other is immersed in the lower edge of the test strip To the weakly acidic solution containing OP (50 pmol / L), Ach (1 mmol / L) and TMB (0.5 mmol / L), after 2-5 min, observe the colors of the two test papers. As attached image 3 As shown, the color of the test strip without OP is darker than the color with OP, indicating that the test strip prepared in this scheme can be used for qualitative detection of OP.

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Abstract

The invention relates to the field of nano catalysis, environment protection and analytical chemistry, in particular to a test strip for rapidly detecting pesticides. Ferroferric oxide nanoparticles with pseudo peroxidase activity are in-situ generated on the test strip, and acetylcholin esterase and choline oxidase are modified on the test strip; when organophosphorus pesticides exist, the activity of the acetylcholin esterase is irreversibly inhibited to ensure that the amount of choline generated by acetyl choline hydrolysis is reduced, so that the hydrogen peroxide generated through cascade reaction is decreased and the developing of chromogenic substrates becomes light. Through a chromatography phenomenon of test paper, interferences of other substances in samples can be eliminated. Though mobile phone photographing and mobile phone software analysis, convenient quantitative detection for acetyl choline can be realized. The test strip can be used for the fields of pesticide residual analysis, biological medicine, environment monitor and the like.

Description

Technical field [0001] The invention relates to the field of analytical chemistry, and specifically includes a test strip for rapid detection of pesticides. technical background [0002] Organophosphorus Pesticide (OP) is the most widely used agricultural pesticide and plays an important role in improving agricultural productivity and preventing pests and diseases. However, organophosphorus pesticides can inhibit the activity of acetylcholinesterase in the human body and cause a variety of human diseases, such as Alzheimer's disease, Parkinson's disease and stroke. At the same time, due to the high residue characteristics of organophosphorus pesticides and the severe overuse or irregular operation in agricultural production, the high content of pesticide residues in agricultural products is caused, which seriously threatens the ecological environment balance. Therefore, the construction of a highly sensitive detection platform to achieve simple, rapid and highly sensitive detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 李峰吴加慧韩磊
Owner QINGDAO AGRI UNIV
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