Optical fibre bio-sensor of acetylcholinesterase and its preparing process

Abstract

An optical fibre bio-sensor for distriminating the nature of peripheral nerve fibres is composed of optical fibre and sensing membrane which is fixed to the end of optical fibre by co-valent binding or physical adsorption. Said sensing membrane is prepared from high-molecular film containing oxygen indicator, choline oxidase and high-molecular membrane by one of three methods. Its advantages include no need of cutting nerve fibre, high speed and correctness and no poison and no wound to human body.

Description

1. Technical field [0001] The invention relates to a sensor for detecting the properties of peripheral nerve bundles and a preparation method thereof, in particular to an acetylcholinesterase optical fiber biosensor and a preparation method thereof. 2. Background technology [0002] In the prior art, there are mainly the following methods for judging the properties of nerve bundles: [0003] 1. Anatomical method: The localization map of the nerve bundles in the nerve trunk has been studied. Many scholars have analyzed the peripheral nerves (such as the median nerve, radial nerve, ulnar nerve trunk, sciatic nerve trunk, etc.) respectively, and observed the nerve trunk. The location, shape, and number of bundles of the nerve were measured, and the local location map of the nerve bundle was drawn. However, in clinical practice, the two ends of the nerve are often twisted after nerve injury, which changes the original normal anatomical position. Clinical application is limited....

AI Technical Summary

Problems solved by technology

However, this method needs to keep the patient awake, and is limited to fresh injuries, individual differences are large, and it is impossible to distinguish whether the muscle contraction caused by stimulation is the result of nerve conduction or current diffusion.

Therefore, the clinical application value is not large

[0005] 3. Radiobiochemical method: use radioactive isotope to label the substrate of the enzyme, and then measure the activity of the enzyme according to the radioactive product produced by the enzyme reaction, and infer the nature of the nerve bundle, but this method involves isotope technology, high equipment requirements, and radiation The substance is harmful to both the patient and the operator, so it is not very practical

However, there are problems with this method: (1) the staining time is long (about 50 minutes), and the process is complicated; (2) two nerve segments need to be cut out, and in situ staining cannot be performed

However, the problem with this method is that the sensor needle must be replaced after each nerve bundle is detected, and cannot be reused. It still takes a few minutes to detect a nerve bundle, which is a bit too long

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