38 results about "Luminol chemiluminescence" patented technology

The invention relates to a gold nanoflower preparing method and the application of gold nanoflowers. The preparing method comprises the following steps that 1) under room temperature, polyphenol is added to water solution with the pH value of 7-12 to obtain mixed solution; 2) chloroauric acid and hydrogen peroxide are then added in sequence, shaking up is carried out to obtain reaction solution; 3) the reaction solution is centrifuged, supernatant is abandoned, and the gold nanoflowers are obtained after washing. The particle diameters of the gold nanoflowers obtained under different pH values are 50-200nm, stability is good, the gold nanoflowers can be used in a biological body as a medicine carrier and have good biocompatibility, the gold nanoflowers can be used in chemiluminescence analysis, the signal intensity of a luminal chemiluminescence system can be obviously enhanced, the gold nanoflowers can be used in surface-enhanced Raman scattering (SERS) analysis, and the sensitivity is better than that of spherical gold nanoparticles. The gold nanoflower preparing method has the advantages that raw materials are easy to get, reaction conditions are mild, efficiency is high, speed is high, and batch preparing and producing are benefited.

The invention relates to a kit for rapidly detecting organophosphorus pesticide residues and a method for using the kit; the kit includes: C enzyme bottle, T enzyme bottle, C substrate reaction cup, T substrate reaction cup, the C enzyme bottle , T enzyme bottles are all sealed with enzyme bottle sealing caps, and are equipped with enzyme freeze-dried three-enzyme mixture; the C substrate reaction cup, T substrate reaction cup are equipped with acetylcholine, luminol and dimethyl Freeze-dried absorbent pads for benzidine. The present invention also relates to a method for rapidly detecting organophosphorus pesticide residues using the aforementioned kit. The invention uses acetylcholinesterase, choline oxidase and peroxidase as a catalytic system to hydrolyze and oxidize the acetylcholine chloride product with luminol to produce a chemiluminescent effect. Establish a method for sensitive testing of organophosphorus pesticides in agricultural products. The invention has the advantages of fast detection speed, simple operation process, good stability and repeatability, and does not require expensive instruments, and is very suitable for on-site monitoring of organophosphorus pesticides in farmers' markets.

The invention discloses a method for enhancing luminol chemoluminescence by using copper oxide nanoparticles and is characterized in that the method comprises the following steps of: firstly pumping a copper oxide nanoparticle colloid solution and a hydrogen peroxide solution into a mixer for mixing through peristaltic pumps respectively, then mixing with a luminol solution, loading the resulting mixture to a flow cell for reaction, and detecting chemoluminescence intensity by a photomultiplier tube. The copper oxide nanoparticles are added to the luminol-hydrogen peroxide system, so as to significantly enhance chemoluminescence signals. The luminol-hydrogen peroxide-copper oxide nanoparticle flow injection chemiluminescence detection can be applied to glucose content determination, with a linear range of 5 mumol / L to 60 mumol / L.

The invention discloses a paper chip with the function of prolonging chemiluminescence. After the reaction, the water-soluble metal-organic complex Cu-MOF was obtained by freeze-drying as a catalyst, and the filter paper was used as a carrier. Cu-MOF was dissolved in a BR buffer solution with a pH of 8-10, and then the solution was dropped on the filter paper. , dried naturally to obtain paper chips. The present invention is aimed at traditional luminol-H that occurs in solution 2 o 2 The chemiluminescence system, by changing the carrier of the reaction—making the reaction occur on the surface of the solid-phase paper, with the help of the unique capillary structure of the paper, affects the contact time between the luminescence system and the catalyst, thereby prolonging the time for the chemiluminescence intensity to reach the maximum value, Realize the effect of delayed glow. The preparation method of the paper chip of the present invention is simple, low in cost, green and environment-friendly, and can be used in the fields of underwater lighting, emergency rescue and the like.

The invention relates to a chemical luminous sensor and a method for detecting glucose by employing the chemical luminous sensor and can be applicable to clinical glucose detection. According to the method, glucose oxidase, a chemical luminous agent (luminol) and a catalyst are simultaneously fixed, and the luminous reaction conditions of the luminol are widened, so that the traditional alkaline substrate is expanded to a weakly acidic substrate, and the sensitivity and service life of the chemical luminous sensor are guaranteed; and moreover, the chemical luminous sensor has the characteristics of easy operation, low cost and wide linear range.

The application provides a luminol chemiluminescence system, which includes luminol, thiourea dioxide and a solvent. The application provides a novel and efficient chemiluminescence system, in which thiourea dioxide and luminol can react to produce chemiluminescence. Thiourea dioxide has good thermal stability, is safe and non-toxic, has no pollution during production and use, has fast chemiluminescent reaction with luminol, and has high luminous intensity. The experimental results show that, compared with the luminol-hydrogen peroxide chemiluminescence system, the maximum luminous intensity of the luminol-thiourea dioxide chemiluminescence system is increased by 20 times.

The invention discloses an enhanced chemiluminescence reagent which is composed of a solution A and a solution B. The solution A comprises 8-15 mmol / L luminol or luminol derivatives, 1-5 mmol / L sodium hydroxide, 0.1-1.5 mmol / L 4-[1H-1,2,4-triazole-1-based methyl] aniline hydrochloride, 0.1-0.8 mmol / L 1-naphthyl boric acid and a 0.1 mol / L Tris-HCl buffer solution with the pH ranging from 7.5 to 10. The solution B comprises 5-10 mmol / L hydrogen peroxide, 0.01%-0.05% of glutamic acid by volume and a 0.1 mol / L Tris-HCl buffer solution with the pH ranging from 7.5 to 10. The enhanced chemiluminescence reagent can remarkably improve the luminous intensity of a luminol chemiluminescence system and prolong the duration of luminescence.