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Luminol chemiluminescence system based on paper matrix and method for detecting phenolic antioxidant

A phenolic antioxidant and luminol chemistry technology, applied in the field of chemiluminescence, can solve the problems of unstable hydrogen peroxide, easy decomposition, large environmental impact, etc., achieving less sample demand, high sensitivity, and less environmental pollution Effect

Active Publication Date: 2020-06-26
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional chemiluminescent system, this method still needs to introduce an additional oxidant hydrogen peroxide, however, hydrogen peroxide is unstable, easy to decompose when heated, and has a relatively large environmental impact

Method used

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  • Luminol chemiluminescence system based on paper matrix and method for detecting phenolic antioxidant
  • Luminol chemiluminescence system based on paper matrix and method for detecting phenolic antioxidant
  • Luminol chemiluminescence system based on paper matrix and method for detecting phenolic antioxidant

Examples

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Embodiment 1

[0027] The luminol chemiluminescent system of this embodiment is composed of 2.5mg / mL CoFe PBA NAFSs aqueous solution, 1.4mmol / L luminol working solution and a paper matrix containing hydrophilic and hydrophobic regions. The luminol working solution is made of Luminol Minol is prepared by sodium hydroxide aqueous solution with a pH value of 11.6, and the paper substrate is made of Whatman No. 1 filter paper.

[0028] Such as figure 1 As shown, the method for detecting caffeic acid using the above-mentioned luminol chemiluminescent substance is as follows:

[0029] 1. Add 20μL 10ng / mL, 20ng / mL, 40ng / mL, 60ng / mL, 80ng / mL, 100ng / mL, 200ng / mL, 400ng / mL, 600ng / mL, 800ng / mL caffeic acid solution (prepared from 95% volume concentration of ethanol aqueous solution), after mixing evenly, take 2.5μL dropwise on the hydrophilic area of ​​the paper substrate, and then add 15μL 1.4mmol / L Lu Minol working solution is injected into the hydrophilic area of ​​the paper substrate by static in...

Embodiment 2

[0031] The luminol chemiluminescence system of this embodiment is composed of 2.0mg / mL Co Fe PBA NAFSs aqueous solution, 1.0mmol / L luminol working solution and a paper matrix containing hydrophilic and hydrophobic regions. The luminol working solution is made of Luminol Minol is prepared by sodium hydroxide aqueous solution with a pH value of 10.0, and the paper substrate is made of Whatman No. 1 filter paper.

[0032] The method for detecting caffeic acid using the above-mentioned luminol chemiluminescent system is the same as that in Example 1.

Embodiment 3

[0034] The luminol chemiluminescent system of this embodiment is composed of 3.0mg / mL Co Fe PBA NAFSs aqueous solution, 1.5mmol / L luminol working solution and a paper matrix containing hydrophilic and hydrophobic regions. The luminol working solution is made of Luminol Minol is prepared by sodium hydroxide aqueous solution with a pH value of 11.6, and the paper substrate is made of Whatman No. 3 filter paper.

[0035] The method for detecting caffeic acid using the above-mentioned luminol chemiluminescent system is the same as that in Example 1.

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Abstract

The invention discloses a luminol chemiluminescence system based on a paper matrix and a method for detecting a phenolic antioxidant. The chemiluminescence system is composed of a Co Fe PBA NAFSs aqueous solution, a luminol working solution and a paper matrix containing hydrophilic and hydrophobic regions, wherein the Co Fe PBA NAFSs are KCoFe self-assembled nano particles with an anisotropic cubic frame structure, and the structural formula of the Co Fe PBA NAFSs is shown in the specification; the concentration of the Co Fe PBA NAFSs aqueous solution is 0.5-3.5 mg / mL, and the luminol workingsolution is a 0.4-1.8 mmol / L luminol solution prepared from a sodium hydroxide aqueous solution of which the pH value is 9.5-12.2. The chemiluminiscence system can catalyze luminol to generate strongchemiluminiscence on a paper substrate without hydrogen peroxide, when the phenolic antioxidant exists in the system, the chemiluminiscence system has an inhibiting effect on chemiluminiscence intensity, and the chemiluminiscence system can be used for detecting the phenolic antioxidant. The chemiluminiscence system has the advantages of rapidness, simplicity, convenience, high sensitivity, smallreagent dosage, small pollution and the like when being used for detecting the phenolic antioxidant.

Description

technical field [0001] The invention belongs to the technical field of chemiluminescence, and in particular relates to a new chemiluminescence system and a method for detecting phenolic antioxidants by using the chemiluminescence system. Background technique [0002] As antioxidants, phenolic compounds are widely found in vegetables and fruits, such as tomatoes, onions, apples and strawberries, as well as beverages such as wine, tea and fruit juices, which are important for improving the antioxidant level in human blood and neutralizing free radicals Yes, it can weaken the destructive effect of active oxygen on the human body. Caffeic acid, also known as 3,4-dihydroxycinnamic acid, as the most important phenolic compound, has been extensively studied for its functions such as antioxidant, anti-inflammatory, antibacterial and immune regulation. Due to these many functions, it has more applications in medicine, food and health products, which can help prevent cancer, heart di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N31/10
CPCG01N21/76G01N31/10
Inventor 刘伟张柳侯越吕聪聪
Owner SHAANXI NORMAL UNIV
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