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A kind of luminol chemiluminescence system and the determination method of luminol, thiourea dioxide, cobalt ion concentration

A technology of thiourea dioxide and luminol chemistry, which is applied in the field of chemical analysis, can solve the problems of unstable hydrogen peroxide, slow chemiluminescence reaction, and low luminescence intensity, and achieves improved luminescence intensity, good thermal stability, and high luminescence intensity. Effect

Inactive Publication Date: 2016-09-28
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing luminol chemiluminescence system, the most commonly used oxidant is hydrogen peroxide, but hydrogen peroxide is unstable, the chemiluminescent reaction with luminol is slow, and the luminous intensity is low

Method used

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  • A kind of luminol chemiluminescence system and the determination method of luminol, thiourea dioxide, cobalt ion concentration
  • A kind of luminol chemiluminescence system and the determination method of luminol, thiourea dioxide, cobalt ion concentration
  • A kind of luminol chemiluminescence system and the determination method of luminol, thiourea dioxide, cobalt ion concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] use figure 1 The system shown measures the concentration of luminol

[0071] Prepare 40mmol / L thiourea dioxide solution, sodium carbonate-sodium hydroxide buffer solution with a pH value of 11.9 and a concentration of 1×10 -9 mol / L, 2×10 -9 mol / L, 5×10 -9 mol / L, 1×10 -8 mol / L, 2×10 -8 mol / L, 5×10 -8 mol / L, 1×10 -7 mol / L, 2×10 -7 mol / L, 5×10 -7 mol / L, 1×10 -6 mol / L, 2×10 -6 mol / L, 5×10 -6 mol / L, 1×10 -5 mol / L, 2×10 -5 mol / L, 5×10 -5 mol / L, 1×10 -4 mol / L luminol solution.

[0072] Add 50 mL of 40 mmol / L thiourea dioxide solution to the first container, add 50 mL of sodium carbonate-sodium hydroxide buffer solution with a pH value of 11.9 to the second container, start the first and second pumping units, and set the flow rate to 1.25 mL / min. In turn, the concentration was 1×10 -9 mol / L, 2×10 -9 mol / L, 5×10 -9 mol / L, 1×10 -8 mol / L, 2×10 -8 mol / L, 5×10 -8 mol / L, 1×10 -7 mol / L, 2×10 -7 mol / L, 5×10 -7 mol / L, 1×10 -6 mol / L, 2×10 -6 mol / L, 5×10 -6 mo...

Embodiment 2

[0076] use figure 1 The system shown measures the concentration of thiourea dioxide

[0077] Prepare an alkaline solution of luminol (10 μmol / L, pH 11.9) and a concentration of 0, 2×10 -6 mol / L, 5×10 -6 mol / L, 1×10 -5 mol / L, 2×10 -5 mol / L, 5×10 -5 mol / L, 1×10 -4 mol / L, 2×10 -4 mol / L, 5×10 -4 mol / L, 1×10 -3 mol / L solution of thiourea dioxide.

[0078] Add 50 mL of an alkaline solution of luminol to the first container, add 50 mL of water to the second container, start the first and second pumping units, and set the flow rate to 1.25 mL / min. Set the concentration to 0, 2×10 in turn -6 mol / L, 5×10 -6 mol / L, 1×10 -5 mol / L, 2×10 -5 mol / L, 5×10 -5 mol / L, 1×10 -4 mol / L, 2×10 -4 mol / L, 5×10 -4 mol / L, 1×10 -3 50 μL of mol / L thiourea dioxide was injected into the injection valve, and the chemiluminescence detector recorded the luminous intensity under different thiourea dioxide concentrations (the voltage of the photomultiplier tube PMT was 1000V).

[0079] Take the c...

Embodiment 3

[0082] use figure 1 The system shown measures cobalt ion concentration

[0083] Preparation of alkaline solution of luminol (10 μmol / L, pH11.9), 0.1mol / L thiourea dioxide solution and concentrations of 0.5nmol / L, 5nmol / L, 10nmol / L, 50nmol / L, 100nmol / L, 200nmol / L, 400nmol / L, 600nmol / L, 800nmol / L, 1000nmol / L cobalt chloride solution, 10μL of the thiourea dioxide solution and 1mL of water were mixed to obtain a blank control sample, and 10μL of the Thiourea dioxide solution and 1 mL of the cobalt chloride solution were mixed to obtain a series of mixed solutions.

[0084] Add 50 mL of an alkaline solution of luminol to the first container, add 50 mL of water to the second container, start the first and second pumping units, and set the flow rate to 1.25 mL / min. The blank control sample and the mixed solution were sequentially injected into the injection valve, each injection volume was 50 μL. The chemiluminescence detector records the luminous intensity under different cobalt ...

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Abstract

The application provides a luminol chemiluminescence system, which includes luminol, thiourea dioxide and a solvent. The application provides a novel and efficient chemiluminescence system, in which thiourea dioxide and luminol can react to produce chemiluminescence. Thiourea dioxide has good thermal stability, is safe and non-toxic, has no pollution during production and use, has fast chemiluminescent reaction with luminol, and has high luminous intensity. The experimental results show that, compared with the luminol-hydrogen peroxide chemiluminescence system, the maximum luminous intensity of the luminol-thiourea dioxide chemiluminescence system is increased by 20 times.

Description

technical field [0001] The invention belongs to the field of chemical analysis, and in particular relates to a luminol chemiluminescence system and a method for measuring the concentrations of luminol, thiourea dioxide and cobalt ions. Background technique [0002] Chemiluminescence refers to the phenomenon of emitting visible light in some special chemical reactions. The luminescence mechanism is that some substances in the reaction system absorb the energy released by the reaction and transition from the ground state to the excited state, and return the energy from the excited state to the ground state. Released in the form of radiation, resulting in luminescence. For a chemical reaction to produce chemiluminescence, the following conditions must be met: first, the reaction must provide sufficient excitation energy, and it must be provided by a certain step alone, because the energy released by the previous step reaction will disappear in the solution due to vibrational re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 徐国宝高文跃
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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