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Chemical luminous sensor and method for detecting glucose by employing chemical luminous sensor

A chemiluminescence and sensor technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems of reduced enzyme activity and inability to fix on the same column, so as to achieve simple operation and broaden the scope of chemistry. Luminescence reaction conditions and effects of cost reduction

Active Publication Date: 2015-04-15
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the reaction pH values ​​of the two are different, if the activity of glucose oxidase is ensured, the use of luminol cannot be ensured. If the condition of luminol is guaranteed, the activity of the enzyme will be greatly reduced, so the two cannot be fixed on the same surface. on a pillar

Method used

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  • Chemical luminous sensor and method for detecting glucose by employing chemical luminous sensor
  • Chemical luminous sensor and method for detecting glucose by employing chemical luminous sensor
  • Chemical luminous sensor and method for detecting glucose by employing chemical luminous sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation method of chemiluminescence sensor:

[0020] A: Weigh 0.03mol of magnesium nitrate and 0.015mol of aluminum nitrate, mix them, add 60mL of pure water, and prepare salt solution; weigh 0.0075mol of sodium carbonate and 0.12mol of sodium hydroxide, mix, add 60mL of pure water, Prepared into lye. Put the above two solutions drop by drop into the four-neck flask, stir, and control the pH value of the mixture between 9.0-10.5. After the dropwise addition, the four-necked bottle was placed in a 60°C water bath for 24 hours of crystallization. The product was centrifuged, washed and dried to obtain magnesium aluminum carbonate hydrotalcite ([Mg 4 Al 2 (OH) 12 ]CO 3 ·yH 2 o).

[0021] B: Take 0.1 g of the above-mentioned magnesium aluminum carbonate hydrotalcite and add 5 mL of luminol solution with a concentration of 0.0002 mol / L, absorb for 10 min, filter, and dry at room temperature to obtain a yellow-green product. The mass ratio of magnesium aluminum ca...

Embodiment 2

[0027] Preparation method of chemiluminescence sensor:

[0028] A: Weigh 0.045mol of magnesium nitrate and 0.015mol of aluminum nitrate, mix them, add 60mL of pure water, and prepare salt solution; weigh 0.0075mol of sodium carbonate and 0.12mol of sodium hydroxide, mix, add 60mL of pure water, Prepared into lye. Put the above two solutions drop by drop into the four-neck flask, stir, and control the pH value of the mixture between 9.0-10.5. After the dropwise addition, the four-necked bottle was placed in a 60°C water bath for 24 hours of crystallization. The product was centrifuged, washed and dried to obtain magnesium aluminum carbonate hydrotalcite ([Mg 6 Al 2 (OH) 12 ]CO 3·yH 2 o).

[0029] B: Take 0.1 g of the above-mentioned magnesium aluminum carbonate hydrotalcite and add 15 mL of luminol solution with a concentration of 0.0002 mol / L, absorb for 10 min, filter, and dry at room temperature to obtain a yellow-green product. The mass ratio of magnesium aluminum c...

Embodiment 3

[0034] Preparation method of chemiluminescence sensor:

[0035] A: Weigh 0.06mol of magnesium nitrate and 0.015mol of aluminum nitrate, mix them, add 60mL of pure water to prepare a salt solution; weigh 0.0075mol of sodium carbonate and 0.12mol of sodium hydroxide, mix them, add 60mL of pure water, Prepared into lye. Put the above two solutions drop by drop into the four-neck flask, stir, and control the pH value of the mixture between 9.0-10.5. After the dropwise addition, the four-necked bottle was placed in a 60°C water bath for 24 hours of crystallization. The product was centrifuged, washed and dried to obtain magnesium aluminum carbonate hydrotalcite ([Mg 8 Al 2 (OH) 12 ]CO 3 ·yH 2 o).

[0036] B: Take 0.2 g of the above-mentioned magnesium aluminum carbonate hydrotalcite and add 25 mL of luminol solution with a concentration of 0.0002 mol / L, absorb for 10 min, filter, and dry at room temperature to obtain a yellow-green product. The mass ratio of magnesium alumi...

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Abstract

The invention relates to a chemical luminous sensor and a method for detecting glucose by employing the chemical luminous sensor and can be applicable to clinical glucose detection. According to the method, glucose oxidase, a chemical luminous agent (luminol) and a catalyst are simultaneously fixed, and the luminous reaction conditions of the luminol are widened, so that the traditional alkaline substrate is expanded to a weakly acidic substrate, and the sensitivity and service life of the chemical luminous sensor are guaranteed; and moreover, the chemical luminous sensor has the characteristics of easy operation, low cost and wide linear range.

Description

Technical field: [0001] The invention relates to a chemiluminescence sensor and a method for detecting glucose by using the sensor. It can be applied to the detection of glucose in clinical practice. Background technique: [0002] Chemiluminescence (CL) refers to the phenomenon that certain chemical reactions emit light, usually redox reactions. Chemiluminescence analysis does not require external excitation light sources, so there is no background interference, which makes it highly sensitive and the detection limit can reach 10 -21 -10 -12 mol. Moreover, the method has a wide linear range (3-6 orders of magnitude), simple equipment, convenient operation, low cost, and easy automation. It has become a very active research hotspot in analytical chemistry. In recent years, due to the advantages of chemiluminescent sensors, which can improve the stability and catalytic activity of catalysts to a certain extent, and are easy to operate, simple in instruments, environmentall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 王志华刘芳吕超
Owner BEIJING UNIV OF CHEM TECH
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