Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting PC content in crude oil through electrochemical double-enzyme sensor

An enzyme sensor, electrochemical technology, applied in the direction of material electrochemical variables, material analysis by electromagnetic means, measuring devices, etc., can solve the problems of high cost, long detection time, complicated operation, etc.

Inactive Publication Date: 2019-11-15
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection methods have disadvantages such as complex operation, long detection time, and high cost. Therefore, the detection of phospholipids in crude oil should start with a single PC, and gradually apply to crude oil containing multiple components.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0006] The method for detecting PC content in crude oil with a self-made electrochemical double-enzyme sensor is realized through the following steps: Step 1: a self-made electrochemical double-enzyme sensor. Take 0.15 g of chitosan (CS) powder into a 250 mL flask, add a 0.3% CS / acetic acid transparent solution, and stir with an electric stirrer for 30 min to promote the full dissolution of CS. A mixture of 2.5 mg of tin dioxide (SnO2) and 5 mg of multi-walled carbon nanotubes (MWCNTs) powder was added to 5 mL of CS / acetic acid transparent solution, and sonicated for 60 min to obtain MWCNTs-SnO2-CS nanocomposite. Store for later use; Step 2: Prepare a PBS buffer solution containing 100 mg·L-1PC as a test base solution. 2.5U horseradish peroxidase solution was drop-coated on the surface of GCE, and left to dry at 4 °C. When the surface became a film, 5 μL of glutaraldehyde was added dropwise to the surface of the electrode, cross-linked at 37 °C for 30 min, and then used Wash ...

specific Embodiment approach 2

[0008] The difference between this embodiment and the first embodiment is that in step one, a CS / acetic acid transparent solution with a concentration of 1% is added, and other steps are the same as those of the first embodiment.

specific Embodiment approach 3

[0010] The difference between this embodiment and the first embodiment is that the crosslinking temperature in the second step is 25° C., and other steps are the same as the first embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting PC content in crude oil through an electrochemical double-enzyme sensor. Choline oxidase and horseradish peroxidase are immobilized on a GCE (glass carbonelectrode) with MWCNTs / SnO2 / CS as a modification material to prepare a double-enzyme GCE; under the conditions that the adding amount of ChOx is 2.5U, the adding amount of HRP is 2.5 U and pH is 7.5,the double-enzyme GCE has remarkable electrochemical response to PC (phosphatidylcholine) and has linear relation with peak current when the PC content is in the range of 10-300 mg / L, the reproducibility RSD of the double-enzyme GCE is lower than 1%, the current value of the double-enzyme GCE after 45 d of preservation is still 93% of initial current, and the prepared double-enzyme GCE has betterreproducibility and stability and is expected to provide a theoretical basis for rapid detection of the content of PC in the crude oil.

Description

technical field [0001] The invention relates to the field of electrochemical rapid detection, in particular to a method for rapidly detecting PC content in crude oil through an electrochemical double-enzyme sensor, that is, a self-made electrochemically modified double-enzyme glassy carbon electrode sensor is used to detect the content of PC in crude oil. PC for efficient and fast detection. Background technique [0002] Crude oil has the highest content of phospholipids in vegetable oils, and PC is the main component. During the oil refining process, if the degumming is insufficient and the minimum standard of phospholipid residue is not reached, it will have a huge impact on the remaining refining process, which will affect the shelf life of the oil and bring about a series of safety issues. Therefore, it is particularly important to detect the content of phospholipids in crude oil. [0003] At present, the traditional detection methods of phospholipids include ultraviol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 于殿宇于长华王旭李丹岳莹雪江连洲王立琦吴非潘明喆
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com