Enzymatic methods for measuring plasma and tissue sphingomylelin and phosphatidylcholine

A technology of phosphatidylcholine and tissue sheath, which can be used in the test method of plasma SM and PC measurement, and the field of enzymatic measurement method, which can solve the problems of time-consuming and insensitive

Inactive Publication Date: 2009-01-28
THE RES FOUND OF STATE UNIV OF NEW YORK
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is time consuming and insensitive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzymatic methods for measuring plasma and tissue sphingomylelin and phosphatidylcholine
  • Enzymatic methods for measuring plasma and tissue sphingomylelin and phosphatidylcholine
  • Enzymatic methods for measuring plasma and tissue sphingomylelin and phosphatidylcholine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] Traditionally, SM and PC are measured in 4 steps: 1) lipid extraction; 2) thin-layer chromatography (TLC); 3) extraction of SM and PC from corresponding spots on the TLC plate, and 4) in individual extracts Quantitative phosphate. The whole process is time-consuming and insensitive. Although a simple method exists (Wako Pure Chemical) for measuring total phospholipids including choline (PC+SM), there is no corresponding method for direct measurement of SM and PC. The present invention relates to two rapid, specific and sensitive assays for the measurement of SM and PC in plasma.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for measuring sphingomyelin and phosphatidylcholine comprising incubating sphingomyelin and phosphatidylcholine with bacterial sphingomyelinase and bacterial phospholipase D, alkaline phosphatase, choline oxidase, peroxidase, N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline, and 4-aminoantipyrine, preferably for about 45 minutes. A blue dye is generated.

Description

Background technique [0001] In addition to cholesterol and triglycerides, lipoproteins also contain phospholipids, of which phosphatidylcholine (PC) and sphingomyelin (SM) are the two main components, the former accounting for about 70% of the total phospholipids and the latter about 20% of total phospholipids. In human case-control studies, it has been shown that both plasma SM and SM / PC ratio are independent risk factors for coronary heart disease. [0002] It has been known for some time that SM accumulates in atherosclerosis in humans and animal models. Low-density lipoprotein (LDL) extracted from human atherosclerotic lesions is much more abundant in SM than LDL from plasma. Plasma SM levels in apoE knockout (apoE KO) mice were 4-fold higher than in wild-type mice, which may partly explain the increased atherosclerosis in these animals. The SM / PC ratio was 5-fold higher in VLDL from hypercholesterolemic rabbits. [0003] Recently, it has been demonstrated that adminis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28
CPCC12Q1/44G01N2405/04G01N2333/916C12Q1/28G01N2405/08C12Q2326/96C12Q1/42
Inventor 江贤成穆罕默德·礼萨·霍贾提
Owner THE RES FOUND OF STATE UNIV OF NEW YORK
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap