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Method for determining organophosphorus

A technology of organophosphorus and organophosphorus pesticides, which is applied in measuring devices, fluorescence/phosphorescence, instruments, etc., can solve unseen problems and achieve the effects of simple operation, simple modification, and good speed

Inactive Publication Date: 2020-11-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these sensors are all based on a single-enzyme reaction, and there is no method to combine a dual-enzyme cascade reaction with upconversion fluorescence quenching for the detection of OPs in food.

Method used

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  • Method for determining organophosphorus

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1. Preparation of Upconversion Nanomaterials

[0039] (1) NaYF coated with oleic acid 4 : Preparation of Yb, Er up-conversion nanomaterials (OA-UCNPs) The specific method for synthesizing OA-UCNPs by high temperature pyrolysis is as follows: Accurately weigh YbCl 3 ·6H 2 O (0.18mmol), YCl 3 ·6H 2 O (0.8mmol) and ErCl 3 ·7H 2 Add O (0.02mmol) into a 100mL three-necked flask, then add 6mL oleic acid (OA) and 15mL octadecene (1-ODE), and gradually raise the temperature to 160°C under the condition of blowing nitrogen and stirring until the reagents are dissolved evenly Then cool down to room temperature naturally. Weigh 4mmol NH 4 F and 2.5mmol NaOH were dissolved in 10mL of methanol solution, and were added dropwise into a three-necked flask, first heated to 60°C and magnetically stirred for 30min, and then gradually heated to 110°C for evaporation to remove methanol and residual water. Add a condensing reflux device, raise the temperature to 300°C, and continue t...

Embodiment 2

[0054] 1. Preparation of Upconversion Nanomaterials

[0055] (1) NaYF coated with oleic acid 4 : Preparation of Yb, Er up-conversion nanomaterials (OA-UCNPs) The specific method for synthesizing OA-UCNPs by high temperature pyrolysis is as follows: Accurately weigh YbCl 3 ·6H 2 O (0.18mmol), YCl 3 ·6H 2 O (0.80mmol) and ErCl 3 ·7H 2 Add O (0.02mmol) into a 100mL three-neck flask, then add 6mL oleic acid (OA) and 14mL octadecene (1-ODE), and gradually raise the temperature to 150°C under the condition of blowing nitrogen and stirring until the reagents are dissolved evenly Then cool down to room temperature naturally. Weigh 4mmol NH 4 F and 2.5mmol NaOH were dissolved in 10mL of methanol solution and added dropwise to a three-necked flask, first heated to 55°C and magnetically stirred for 45min, then gradually heated to 110°C for evaporation to remove methanol and residual water. Add a condensing reflux device, raise the temperature to 305°C, and continue the reaction f...

Embodiment 3

[0061] 1. Preparation of Upconversion Nanomaterials

[0062] (1) NaYF coated with oleic acid 4 : Preparation of Yb, Er up-conversion nanomaterials (OA-UCNPs) The specific method for synthesizing OA-UCNPs by high temperature pyrolysis is as follows: Accurately weigh YbCl 3 ·6H 2 O (0.18mmol), YCl 3 ·6H 2 O (0.8mmol) and ErCl 3 ·7H 2 Add O (0.02mmol) into a 100mL three-necked flask, then add 6mL oleic acid (OA) and 17mL octadecene (1-ODE), and gradually raise the temperature to 155°C under the condition of blowing nitrogen and stirring until the reagents are dissolved evenly Then cool down to room temperature naturally. Weigh 4mmol NH 4 F and 2.5mmol NaOH were dissolved in 10mL of methanol solution and added dropwise to a three-necked flask, first heated to 50°C and magnetically stirred for 60min, then gradually heated to 110°C for evaporation to remove methanol and residual water. Add a condensing reflux device, raise the temperature to 310°C, and continue the reaction ...

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Abstract

The invention discloses a method for determining organophosphorus, particularly relates to a method for determining organophosphorus pesticide by double-enzyme mediated Fe<3+> quenching up-conversionfluorescence, and belongs to the technical field of fluorescence analysis. The specific detection method comprises the following steps: uniformly mixing an organophosphorus solution and an acetylcholine esterase (AChE) solution, incubating, adding the acetylcholine (ACh) solution and a choline oxidase (ChOx) solution to obtain an enzyme reaction solution, sequentially adding the enzyme reaction solution and a bivalent iron salt solution into a PAA-UCNPs nano material solution, reacting in a dark place, determining the fluorescence intensity of the reaction solution after the reaction is finished, and determining the organophosphorus content according to the fluorescence intensity. The method is sensitive, reliable and accurate, and has a great application prospect in the field of food safety organophosphorus pesticide detection.

Description

technical field [0001] The invention belongs to the field of food safety detection, relates to a method for measuring organic phosphorus, especially relates to the method of combining double enzyme cascade reaction with Fe 3+ Quenching upconverting fluorescence binding enables quantitative detection of organophosphorus in food samples. Background technique [0002] In industry and agriculture, organophosphorus pesticides (Organophosphorus pesticides, OPs) are widely used to control pests and increase crop yields. OPs have a variety of digestion pathways such as volatilization, photolysis, and microbial degradation, and their residues in the environment have certain persistence. Excessive use and improper disposal of OPs cause excessive residues in the environment, causing air, water and soil pollution. Using fruits and vegetables as food medium, OPs can easily enter the human body through the biological chain. The acute toxicity of OPs is manifested in the inhibition of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N2021/6432
Inventor 吴世嘉林先锋虞倩茹段诺
Owner JIANGNAN UNIV
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