CoOOH NPs-based AChE activity detection test strip and preparation method thereof

A technology of activity detection and test strips, which is applied in the field of biosensors, can solve the problems of high AChE concentration requirements, cumbersome test methods, and low detection sensitivity, and achieve the effects of low cost, easy operation, and simple construction process

Active Publication Date: 2018-12-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of AChE activity detection based on CoOOH NPs in order to overcome the problems of long test time, large error, high requirement for AChE concentration, cumbersome test method and low detection sensitivity in the prior art. test strip

Method used

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  • CoOOH NPs-based AChE activity detection test strip and preparation method thereof
  • CoOOH NPs-based AChE activity detection test strip and preparation method thereof
  • CoOOH NPs-based AChE activity detection test strip and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: Synthesis of CoOOH NPs

[0017] 300 μL NaOH deionized aqueous solution (1.0mol L -1 ) into 1000μL CoCl 2 (10mmol L -1 ) in deionized aqueous solution, the mixture was sonicated for 1 minute; 50 μL NaClO (0.9mol L -1 ) solution was added to the above NaOH and CoCl 2 The mixed solution was sonicated for 10 minutes. Then 650 μL of deionized water was added, and after centrifugation for 10 minutes, the CoOOH NPs were collected and weighed as 1.5 mg after drying. The obtained CoOOH NPs were washed 3 times with deionized water. Finally, the CoOOH NPs were diluted to 25 μg mL with deionized water -1 spare.

Embodiment 2

[0018] Embodiment 2: the making of test strip

[0019] Immerse the test strip (Hangzhou Fuyang Special Paper Co., Ltd., 1cm*1cm) into 25 μg mL dissolved in deionized water -1 CoOOH NPs, 50mmol L -1 ACh and 2.0U mL -1 In the mixed solution of CHO for 3 hours, the AChE activity detection test strip based on CoOOH NPs of the present invention was obtained.

[0020] Take out the test strip and then add it to a concentration of 20mU mL -1 AChE deionized water solution for 15 minutes, finally take out the test strip and dry it, when drop 50μL color developer TMB (1mg mL -1 ), the color reaction is triggered, and the test strip turns blue and can keep the color stable within 25 minutes. The production of reagent strips and the color development process are as follows: figure 2 (a) shown.

Embodiment 3

[0021] Embodiment 3: the application of test strip to the detection of AChE activity

[0022] The present invention has prepared AChE with different concentrations (0,1.25,2.5,5.0,10 and 20mU mL -1 ) of CHO / CoOOHNPs / ACh system test strips, and then drop 50μL 1mg mL -1 After TMB chromogen, the color of the test paper changes from colorless to dark blue with the increase of AChE concentration, as can be observed with the naked eye. figure 2 (b) shown.

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Abstract

The invention relates to a CoOOH NPs-based AChE activity detection test strip and a preparation method thereof, and belongs to the technical field of a biological sensor. The test strip is loaded witha CoOOH NP material, choline oxidase, acetylcholine and a color developing agent. The acetylcholine can be catalyzed to be hydrolyzed by the AChE to generate choline, the choline is further oxidizedto generate hydrogen peroxide by the choline oxidase, the formed hydrogen peroxide is catalyzed and oxidized to generate hydroxyl radical by CoOOH, and the color developing agent is further developed.By the test strip built according to the principle, the AChE can be rapidly detected, the test strip has the advantages of high sensitivity, low cost and the like and is simple and convenient to operate, and a new method is provided for immediate test on the activity of the AChE.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to an acetylcholinesterase (AChE) activity detection test strip based on cobalt oxyhydroxide nanosheets (CoOOHNPs) and a preparation method thereof. Background technique [0002] Acetylcholinesterase is an important serine esterase in the human body, widely present in neuromuscular junctions and cholinergic chemical synapses. As a degrader, it can specifically regulate the level of neurotransmitter acetylcholine. Low levels of acetylcholine can lead to the termination of neurotransmission at cholinergic synapses, leading to the development of Alzheimer's and Parkinson's diseases. Acetylcholinesterase plays an important role in the degradation of the above-mentioned cholinergic system, so highly sensitive assays and on-site screening of acetylcholinesterase have received increasing attention in the field of disease prevention. Then many detection methods came into being, such as...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 卢革宇金蕊孙鹏闫旭刘方猛王晨光高原梁喜双刘凤敏
Owner JILIN UNIV
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