CoOOH NPs-based AChE activity detection test strip and preparation method thereof
A technology of activity detection and test strips, which is applied in the field of biosensors, can solve the problems of high AChE concentration requirements, cumbersome test methods, and low detection sensitivity, and achieve the effects of low cost, easy operation, and simple construction process
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Embodiment 1
[0016] Example 1: Synthesis of CoOOH NPs
[0017] 300 μL NaOH deionized aqueous solution (1.0mol L -1 ) into 1000μL CoCl 2 (10mmol L -1 ) in deionized aqueous solution, the mixture was sonicated for 1 minute; 50 μL NaClO (0.9mol L -1 ) solution was added to the above NaOH and CoCl 2 The mixed solution was sonicated for 10 minutes. Then 650 μL of deionized water was added, and after centrifugation for 10 minutes, the CoOOH NPs were collected and weighed as 1.5 mg after drying. The obtained CoOOH NPs were washed 3 times with deionized water. Finally, the CoOOH NPs were diluted to 25 μg mL with deionized water -1 spare.
Embodiment 2
[0018] Embodiment 2: the making of test strip
[0019] Immerse the test strip (Hangzhou Fuyang Special Paper Co., Ltd., 1cm*1cm) into 25 μg mL dissolved in deionized water -1 CoOOH NPs, 50mmol L -1 ACh and 2.0U mL -1 In the mixed solution of CHO for 3 hours, the AChE activity detection test strip based on CoOOH NPs of the present invention was obtained.
[0020] Take out the test strip and then add it to a concentration of 20mU mL -1 AChE deionized water solution for 15 minutes, finally take out the test strip and dry it, when drop 50μL color developer TMB (1mg mL -1 ), the color reaction is triggered, and the test strip turns blue and can keep the color stable within 25 minutes. The production of reagent strips and the color development process are as follows: figure 2 (a) shown.
Embodiment 3
[0021] Embodiment 3: the application of test strip to the detection of AChE activity
[0022] The present invention has prepared AChE with different concentrations (0,1.25,2.5,5.0,10 and 20mU mL -1 ) of CHO / CoOOHNPs / ACh system test strips, and then drop 50μL 1mg mL -1 After TMB chromogen, the color of the test paper changes from colorless to dark blue with the increase of AChE concentration, as can be observed with the naked eye. figure 2 (b) shown.
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