Stable and strong anti-interference phospholipid detection reagent and detection method thereof

Abstract

The present invention relates to the technical field of phospholipid detection, particularly to a phospholipid detection reagent. According to the present invention, a sodium citrate buffer solution and a Bis-Tris Propane (1,3-bis[tris(hydroxymethyl)methylamino]propane) buffer solution are respectively used in reagents R1 and R2, wherein the Bis-Tris Propane buffer solution used in the reagent is a biological buffer solution, provides good protection effects for the peroxidase used in the reagent, and does not adversely affect the reaction system, the reagent R1 contains phospholipase D, DAOS, a Triton X-305 solution, Emulgen 707, a reducing protection agent, a chelating protection agent and a preservative, and the reagent R2 contains a buffer solution, 4-aminoantipyrine, peroxidase, choline oxidase, and a preservative; and the phospholipid detection reagent has advantages of good stability and low cost, and can be widely used in the field of phospholipid detection.

Description

Technical field [0001] The invention relates to the field of phospholipid detection, in particular to a detection reagent and a detection method for detecting phospholipids using an oxidase method. Background technique [0002] Phospholipid, also known as phospholipid and phospholipid, refers to lipids containing phosphoric acid and is a compound lipid. Phospholipids are the main components of biological membranes. They are divided into glycerophospholipids and sphingomyelin, which are composed of glycerol and sphingosine respectively. Phospholipids are amphiphilic molecules with a hydrophilic nitrogen or phosphorus-containing head at one end and a hydrophobic (lipophilic) long hydrocarbon-based chain at the other end. For this reason, the hydrophilic ends of the phospholipid molecules are close to each other, and the hydrophobic ends are close to each other. They often form a lipid bilayer together with other molecules such as proteins, glycolipids, and cholesterol, that is, th...

AI Technical Summary

Problems solved by technology

Among them, chromatography and mass spectrometry detection methods are the most accurate, but these two methods have high requirements for instruments and operators, and the cost of use is too expensive, so they are not suitable for large-scale promotion

The chemical cost method is relatively low, but this method is not accurate and is susceptible to interference

Enzyme method is relatively common in the market. This method is mainly carried out by means of TRINDER reaction, using phospholipase and choline oxidase to generate H2O2, and using chromogen to perform color formation reaction. This method is widely used, cheap and easy to operate. It is suitable for the supporting use of automatic biochemical analysis instruments, but because this method involves many enzymes and the inherent weak anti-interference ability of the TRINDER reaction itself, most of the existing phospholipid detection reagents on the market are unstable and Problems such as poor anti-interference ability affect normal use

Images