Calorimetric method for detecting pesticides by using acetylcholin esterase

Abstract

The invention relates to a calorimetric method for detecting pesticides by using acetylcholin esterase. According to the technical scheme, an enzymatic inhibition method and cascade reaction are adopted; the acetylcholin esterase is suppressed by pesticides, so that the enzymatic reaction rate is reduced; the quantity of choline products is reduced and is in direct proportion to the suppression degree; and furthermore, the amount of the choline can be obtained by cascade reaction heat of choline oxidase and catalase. By using the cascade reaction, the intensity of an original thermal signal as well as the detection sensitivity and the detection resolution are greatly improved, the detection limit is reduced, and the detection accuracy is effectively improved; a pesticide detection calorimetric method based on the acetylcholin esterase is realized; the advantages of high sensitivity of the acetylcholin esterase to organophosphorus and carbamic acid ester type pesticides and no interference caused by electrochemical active substances or colors and turbidity of the sample on the calorimetric method are integrated; the in-site quick pesticide residue detection is facilitated; and the large-scale application is facilitated.

Description

technical field [0001] The invention relates to a calorimetric method for rapidly detecting pesticides by using acetylcholinesterase, in particular to an acetylcholinesterase calorimetric analysis method capable of realizing thermal amplification, and belongs to the field of pesticide detection. Background technique [0002] Calorimetry is analyzed by measuring the heat change in the biochemical reaction process. Compared with optical and electrochemical methods, it has strong versatility and is not interfered by the color, turbidity, and electrochemical properties of the sample itself, and can meet the requirements of actual samples. It is especially suitable for rapid on-site testing, and the detection principle is simple and reliable, which is conducive to the development of micro-systems. [0003] Acetylcholinesterase (AchE) is essential for the normal nerve conduction activities of organisms. Because organophosphate and carbamate pesticides can inhibit the normal functi...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the heat that acetylcholinesterase enzymatic reaction produces is less, be used for the shortcoming that calorimetry detection difficulty is big and detection sensitivity is low; Realize the high sensitivity of acetylcholinesterase to organic phosphorus and carbamate pesticides and The calorimetry method is suitable for the common advantages of rapid detection of actual samples on the spot; it provides a calorimetric method for pesticide detection based on the large heat release of acetylcholinesterase and is easy to measure to meet the needs of rapid on-site measurement and large-scale application

Images