153 results about "Candida pelliculosa" patented technology

The invention discloses a microbial deodorizing inoculant which is used in refuse sites, public toilets and livestock / poultry cultivation farms. The microbial deodorizing inoculant contains Candida utilis of which the collection number is ACCC 20060, Lactobacillus plantarum of which the collection number is ACCC 11016, lactic streptococci of which the collection number is ACCC 10653, cerevisiae fermentum of which the collection number is ACCC 21135, Streptococcus thermophilus of which the collection number is ACCC 10651, Bacillus licheniformis of which the collection number is ACCC 01870, and Bacillus natto of which the collection number is ACCC 19833. The microbial deodorizing inoculant absorbs and degrades generated malodorous gas hydrogen sulfide and ammonia gas, eliminates the malodor of the garbage and garbage leachate, inhibits the putrefaction bacteria from growth, basically prevents the malodorous gas from generation, and forms a disease-spread-free refuse piling environment capable of eliminating the malodor.

Methods and compositions useful in the detection and identification of species of Candida are disclosed. These species include Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, each of which is a causative agent for vaginal candidiasis. The compositions of the invention are combinations of oligonucleotides. These oligonucleotides include pairs of forward and reverse primers for polymerase chain reactions, wherein each primer pair is capable of priming the synthesis of an amplicon specific to one of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, but preferably is not capable of priming the synthesis of an amplicon specific to any of the other three species. In preferred embodiments, the forward primers of the primer pairs have identical sequences, while each reverse primer of the primer pairs has a unique sequence relative to all of the other reverse primers; or the reverse primers of the primer pairs have identical sequences, while each forward primer of the primer pairs has a unique sequence relative to all of the other forward primers. These unique primer sequences account for the species specificity of the resultant amplicons. The oligonucleotides also include probes capable of detecting these amplicons, and sequencing primers for determining, in primer extension reactions, the nucleotide sequences contained within the amplicons. In preferred methods of the invention, a biological sample is tested for the presence of at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis by isolating nucleic acid from the sample, attempting a polymerase chain reaction in a mixture containing this nucleic acid and a plurality of these primer pairs, ascertaining whether any amplicon is produced in the mixture using an oligonucleotide probe, and determining the sequence of any resultant amplicon using the sequencing primers. The detection of an amplicon indicates that the sample contains at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, or Candida tropicalis, and the nucleotide sequence data is used to determine which of these four Candida species is present.

The invention discloses a candida lipolytica strain and a method for preparing long chain dicarboxylic acid by using the candida lipolytica strain, belonging to the field of microbial fermentation. The candida lipolytica strain is candida lipolytica CX-998 CCTCC NO:M2011094. The method for preparing the long chain dicarboxylic acid comprises the following steps of: in a culture medium in which C10-C18 n-alkane is used as a substrate, using the candida lipolytica strain to perform fermentation; and separating, extracting and recycling the fermentation product to obtain the long chain dicarboxylic acid which is the same as an n-alkane substrate chain. According to the method, all sorts of single and mixed binary acids of C10-C18 can be produced, wherein in a 10-liter fermentation tank, when DC11 is produced from nC11 through fermentation transformation, the DC11 output in 144 hours reaches 165g / L, and the after-treatment yield is 88%; and when the purity of the nC11 is more than 99%, the purity of the DC11 reaches 99%.

The invention discloses an ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin. The ecological restoration microbial inoculum consists of bacillus subtilis, rhodopseudomonas palustris, enterococcus faecalis, wautersiellfalsenii and candida utilis which are mixed together. A preparation method of the ecological restoration microbial inoculum comprises the following steps: inoculating strains to corresponding sterilized culture medium for fermentation; performing three-stage amplification culture; and finally, adsorbing and solidifying on straw charcoal to prepare a solid microbial inoculum. The ecological restoration microbial inoculum disclosed by the invention has the advantages of being high in density and activity and excellent in characteristic. The bacillus subtilis, the rhodopseudomonas palustris, the enterococcus faecalis and the newly separated wautersiellfalsenii have an effect of inhibiting mildews and degrading mycotoxin, and particularly by cooperation with the candida utilis, the effect of degrading mycotoxin is obviously increased. The ecological restoration microbial inoculum can be used as a feed additive to solve the problem of mildew and mycotoxin pollution in the feed industry, and can also be used as a soil ecological restoration agent to solve the problem of the spreading of a continuous cropping disease in plantation of ginseng and notoginseng.

The invention relates to a composite fungicide for treating sludge and a sludge treatment method. The composite fungicide is prepared from the following components in parts by weight: 3 to 8 parts of purple sulfur bacteria, 3 to 8 parts of green sulfur bacteria, 3 to 8 parts of rhodopseudomonas, 3 to 8 parts of rhodopseudomonas capsulata, 3 to 8 parts of rhodococcus rhodochrous, 3 to 8 parts of white-rot fungi, 3 to 8 parts of phosphorus-accumulating bacteria, 3 to 8 parts of anaerobic ammonium oxidation bacteria, 3 to 8 parts of lactobacillus plantarum, 3 to 8 parts of streptomycete, 3 to 8 parts of candida utilis, as well as saccharomyces cerevisiae, lactobacillus lactis, lactobacillus acidophilus, aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus licheniformis.

The invention provides Candida with a nematocidal activity as well as a preparation method and application of Candida and belongs to the technical field of microbial pesticides. The Candida is Candida krusei TY9 and is preserved in the typical culture preservation center in China, and the preservation number of the Candida is CCTCCNO: M2014435. The Candida krusei TY9 strain is prepared by fermentation culture of conventional liquid, is applied to prevention and control of plant-parasitic nematodes, and particularly has a good effect of preventing and controlling panagrellus redivivus and / or bursaphelenchus xylophilus. The Candida has the advantages of low cost, simple preparation process, easiness in raw material obtaining, good in effect of killing nematodes and the like, and is suitable for industrial popularization and application.