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Method for preparing autolysate of candida utilis

A technology for producing Candida utilis and autolysate, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low degree of hydrolysis of proteins and nucleic acids, and achieve the effect of major application prospects.

Inactive Publication Date: 2007-10-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The above method only uses the yeast's own enzyme system, and the degree of hydrolysis of protein and nucleic acid is not high, so exogenous enzymes need to be added. At the same time, because no alcohol is added, the flavor of the autolyzate has a certain yeast odor, so it is necessary to Development of Yeast Extract Products with Good Flavor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Medium preparation:

[0029] Soybean juice slant medium composition: Weigh a certain amount of soybeans, add 10 times the mass of water to soak overnight, boil for 2 hours, and filter with gauze to obtain soybean juice; add sucrose to a final concentration of 30g / L, and agar to a final concentration of 20g / L. Soybean juice slant medium.

[0030] The composition of the wort slant medium: Weigh a certain amount of malt granules, add 4 times the quality of water to soak overnight, crush and refine the pulp, saccharify in a constant temperature water bath at 72°C for about one hour, and test with iodine solution until the saccharification is complete , boiled for 30 minutes to inactivate the enzyme, centrifuged to obtain wort, and then add agar with a final concentration of 20g / L to be the wort slant medium.

[0031] Composition of wort liquid medium: Weigh a certain amount of malt granules, add 4 times the mass of water to soak overnight, crush and refine the pulp, sacchari...

Embodiment 2

[0034] The final concentration of the symbiotic medium consists of: bran 10g / L, soybean meal 30g / L, wort 100g / L, yeast extract 1.5g / L, KH 2 PO 4 4g / L, MgSO 4 ·7H 2 O 1.1g / L; Liquid volume 300mL in 1.5L Erlenmeyer flask, adjust pH7.0, sterilize, cool to room temperature, insert 60mL of the Aspergillus oryzae spore suspension obtained in Example 1, 30 ℃, 120r / min shaking culture 48h Then, insert 25 mL of the Candida utilis seed solution obtained in Example 1, and culture it with shaking at 30° C. and 180 r / min for 24 hours. After the fermentation is finished, filter and collect the mycelium by centrifugation, and the endogenous protease activity is 85 U / g. bacteria.

Embodiment 3

[0036] Get 20g of the mycelium obtained in Example 2 (endogenous enzyme activity 82U / g dry bacteria) and place it in an insulated container with stirring, adjust the solids concentration to 20%, adjust the pH to 5.8, keep the temperature at 50 ± 2°C, add 2.5g sodium chloride, and add 5g rice wine (commercially available Shaoxing rice wine containing 16% alcohol), and 0.1g thiamine and 2g glucose, autolyze 24 hours under stirring, obtain described Candida utilis autolysis Soluble matter, wherein protein peptide content is 38%, and free amino acid content is 8.5%. The total content of four kinds of nucleotides is 3.1%, respectively: guanylic acid (G) = 1.4%, inosinic acid (I) = 1.2%, adenine nucleotide (A) = 0.3%, cytosine nucleoside Acid (C) = 0.19%.

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Abstract

This invention provides a method for preparing Candida utillis autolysate. The method comprises: placing the co-cultured product (endogenous enzyme activity is 60-100 U / g dry fungi) of Candida utillis and Aspergillus oryzae into an insulating container with a stirrer, adjusting the concentration of the solid to 10-30 wt.% of the yeast solution, adding 0.2-2.5 wt.% of NaCl into the yeast solution at a pH value of 4.5-6.8 and a temperature of 45-55 deg.C, then adding 1-5 wt.% of yellow rice wine or white spirit, 0.1 wt.% of thiamine and 2 wt.% of glucose into the yeast solution, and autolyzing under stirring for 24 h to obtain Candida utillis autolysate. The obtained Candida utillis autolysate is a kind of natural flavoring, and can be widely used in foods, healthcare products and industrial culture media.

Description

(1) Technical field [0001] The present invention relates to a kind of preparation method of Candida utilis autolysate, especially a kind of not adding protease and nuclease hydrolase, utilizing the endogenous enzyme system produced by yeast and Aspergillus oryzae symbiosis to prepare yeast effective degradation product (such as nucleus Nucleotides, peptides and amino acids, etc.) (2) Background technology [0002] Yeast is rich in protein, amino acid, multivitamins and trace elements. It is a safe microorganism that has been used in the food industry for a long time. Physiologically active substances such as proteases, digestive enzymes, nucleotides, and B vitamins secreted by yeast; yeast autolysates contain a variety of amino acids, peptides, flavor nucleotides, B vitamins, unique aromatic substances and The main components of the cell wall, such as dextran and mannan oligosaccharides, have two functions of nutrition and seasoning. Therefore, yeast autolysate has a wide ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/16C12R1/69C12R1/72
Inventor 丁玉庭聂小华刘书来
Owner ZHEJIANG UNIV OF TECH
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