Method for preparing animal source-free sauce-fragrant meat flavor from soybean hydrolysate
A technology of soybean hydrolyzate and meat flavor essence, which is applied in the field of soy sauce flavor meat flavor essence, can solve the problems that no patent publications have been found, and achieve the effect of simple method, convenient operation and strong meat flavor
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Embodiment 1
[0023] A method for preparing soy-flavored meat flavor without animal origin by using soybean hydrolyzate, the steps are as follows:
[0024] (1) Preparation of soybean hydrolyzed concentrated solution: use a pulverizer to break soybean meal into a fine powder of 60 mesh, add a 1mol / L hydrochloric acid solution 3 times the weight of the soybean meal powder, treat at 121°C for 2h, and use 5mol / L hydrogen after cooling down to room temperature Adjust the pH of sodium oxide solution to 4.5, add 1% (based on the weight of soybean meal powder) cellulase and 1% (based on the weight of soybean meal powder) hemicellulase, enzymatically hydrolyze at 50°C for 2 hours, and heat up to 90°C to inactivate the enzyme for 15 minutes; Then adjust the pH to 6.0 with 5mol / L sodium hydroxide solution, add 1% (calculated by the weight of soybean meal powder) papain and 1% (calculated by the weight of soybean meal powder) flavor protease, enzymatically hydrolyze at 55℃ for 3h, heat up to 90℃ Enzyme fo...
Embodiment 2
[0028] A method for preparing soy-flavored meat flavor without animal origin by using soybean hydrolyzate, the steps are as follows:
[0029] (1) Preparation of soybean hydrolyzed concentrate: use a pulverizer to pulverize soybean meal into a fine powder of 80 mesh, add 0.5 mol / L hydrochloric acid solution 5 times the weight of soybean meal powder, treat at 115°C for 2.5 hours, and use 5 mol / L after cooling down to room temperature Adjust the pH to 5.0 with L sodium hydroxide solution, add 2% (based on the weight of soybean meal powder) cellulase, enzymatically digest at 55°C for 2.5 hours, heat up to 95°C to inactivate the enzyme for 10 minutes; then adjust the pH with 5mol / L sodium hydroxide solution To 6.5, add 1% (based on the weight of soybean meal powder) alkaline protease and 1% (based on the weight of soybean meal powder) flavour protease, enzymatically hydrolyze at 50°C for 4 hours, heat up to 95°C to inactivate the enzyme for 10 minutes, to obtain soybean hydrolysate;
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Embodiment 3
[0033] A method for preparing soy-flavored meat flavor without animal origin by using soybean hydrolyzate, the steps are as follows:
[0034] (1) Preparation of soybean hydrolyzed concentrate: use a pulverizer to crush soybean meal into a fine powder of 70 mesh, add 1.5mol / L hydrochloric acid solution 4 times the weight of soybean meal powder, treat at 118°C for 3h, and use 5mol / L after cooling down to room temperature Adjust the pH to 5.5 with sodium hydroxide solution, add 2% (based on the weight of soybean meal powder) hemicellulase, enzymatically hydrolyze at 45°C for 3 hours, heat up to 85°C to inactivate the enzyme for 15 minutes; then use 5mol / L sodium hydroxide solution to adjust the pH to 7.0, add 1% (based on the weight of soybean meal powder) papain and 1% (based on the weight of soybean meal powder) compound protease, enzymatically hydrolyze at 60°C for 3.5 hours, and heat up to 85°C to inactivate the enzyme for 15 minutes to obtain soybean hydrolysate;
[0035] The soy...
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