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Method for producing glutathione through Candida utilis fermentation

A technology of Candida utilis and glutathione, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc.

Active Publication Date: 2010-11-10
CHENGDU ZIHAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Chinese patent CN200810019761.6 is a glutathione fermentation process, it also discloses a method for producing glutathione by using genetically engineered methanol-utilizing yeast. The glutathione output can reach 6.6g / L, but it is different from There is still a long way to go for the international level of 7-10g / L (Kyowa Fermentation in Japan)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] According to the GSH fermentation conditions described in the manual, no protein hydrolyzate was added to the culture medium of the shake flask fermentation, and the experimental results of not adding complex vitamin B and L-methionine or L-cysteine:

[0050] GSH production rate 26.0mg / L / h;

[0051] GSH content 2.0g / L

[0052] Reduced GSH85%.

Embodiment 2

[0054] According to the GSH fermentation conditions described in the instructions, 20 g / L of protein hydrolyzate (10 g / L of animal protein hydrolyzate and 10 g / L of plant protein hydrolyzate) were added to the culture medium of shake flask fermentation, and complex vitamin B and Experimental results of L-methionine or L-cysteine ​​2g / L:

[0055] GSH production rate 129.0mg / L / h;

[0056] GSH content 9.7g / L

[0057] The reduced GSH is 98.6%.

Embodiment 3

[0059] According to the GSH fermentation conditions described in the manual, no protein hydrolyzate was added to the culture medium of the shake flask fermentation, and the experimental results of adding 1g / L of complex vitamin B and 2g / L of L-methionine or L-cysteine ​​were added dropwise :

[0060] GSH production rate 45.0mg / L / h;

[0061] GSH content 3.5g / L

[0062] The reduced GSH is 96%.

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Abstract

The invention discloses a method for producing glutathione through microbial fermentation, which is a method that protolysate and vitamin B complex are added in fermentation medium, and inorganic sulfur source is modified into organic sulfur source to greatly improve the yield of glutathione. The invention adopts Candida utilis as glutathione to produce bacterial strain, wherein the yield of glutathione in the fermentation medium containing protolysate, vitamin B complex and organic sulfur reaches 7 to 10 g / L, and the reduced form is larger than 98 percent. The invention has the advantages that the method greatly improves the yield of glutathione under the same other culture conditions, greatly reduces the production cost, and can change the situation of dependence of imported glutathione.

Description

technical field [0001] The invention relates to a method for fermenting and producing glutathione by using Candida utilis. technical background [0002] Glutathione (GSH) is a tripeptide compound composed of glutamic acid, cysteine ​​and glycine. It is an important physiologically active substance in organisms, especially playing a very important role in maintaining the redox environment in organisms. role. Glutathione is a small molecular peptide substance containing sulfhydryl groups, which has two important antioxidant effects and integrated detoxification effects. The sulfhydryl group on cysteine ​​in the molecular structure of glutathione is its active group (so glutathione is often abbreviated as G-SH), and it is easy to react with iodoacetic acid, mustard gas (a poisonous gas), lead, mercury, arsenic and other heavy metal salt complexes, and has an integrated detoxification effect. Glutathione (especially glutathione in liver cells) has a very important physiologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/72
Inventor 何璧梅汤华钊刘忠荣肖友元吴四灵李玲
Owner CHENGDU ZIHAO PHARMA
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