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Candida lipolytica strain and method for preparing long chain dicarboxylic acid by using candida lipolytica strain

A technology of Candida lipolytica and long-chain dibasic acid, applied in the field of microbial fermentation

Active Publication Date: 2011-09-21
山东华星环保集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In a series of patent documents on long-chain dibasic acids at home and abroad represented by Chen Yuantong, Institute of Microbiology, Chinese Academy of Sciences, there are many uses of Candida tropicalis to produce twelve-carbon, thirteen-carbon, fourteen-carbon, fifteen-carbon, sixteen-carbon and Heptadecadioic acid has been reported, but there are few reports on microbial fermentation conversion of high-yield undecanedioic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Take an inoculated Candida lipolytica CX-998 strain, spread it on the wort solid slope of ￠15×180 large test tube and cultivate it at 28°C for 44 hours; In a 500ml Erlenmeyer flask with 300ml of wort juice (12Be' brix) seed medium, cultivate it on a rotary shaker at 200rpm at 28°C for 2 days.

[0017] (2) in 18ml containing C 11 In the 500ml Erlenmeyer flask of the mixed solution of n-alkane and fermentation medium, insert 4ml above-mentioned seed solution, ferment on the rotary shaker of 200rpm for 4 days, adjust pH to 7.5 once every 24 hours with NaOH solution. The C 11 The composition of the mixture of n-alkane and fermentation medium is: nC 11 200ml / L (purity>99%), NaAC 12g / L, KH 2 PO 4 9g / L, NaCl 1.1g / L, yeast extract 3g / L, corn steep liquor 2.5g / L, urea 1.2g / L, sucrose 13g / L, vitamin C lg / L, vitamin B 20.02g / L, prepared with tap water, pH7.2, sterilized at 110°C for 30 minutes. After the fermentation, the pH was adjusted to 3 with HCl. Extract twice wit...

Embodiment 2

[0019] nC 13 (purity is 99.0%), all the other steps are with embodiment 1, result DC 13 Yield is 65.4g / L, DC 13 The purity is 98.5%.

Embodiment 3

[0021] nC 15 (purity is 98.5%), all the other steps are with embodiment 1, result DC 15 Yield is 54g / L, DC 15 The purity is 98.3%.

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Abstract

The invention discloses a candida lipolytica strain and a method for preparing long chain dicarboxylic acid by using the candida lipolytica strain, belonging to the field of microbial fermentation. The candida lipolytica strain is candida lipolytica CX-998 CCTCC NO:M2011094. The method for preparing the long chain dicarboxylic acid comprises the following steps of: in a culture medium in which C10-C18 n-alkane is used as a substrate, using the candida lipolytica strain to perform fermentation; and separating, extracting and recycling the fermentation product to obtain the long chain dicarboxylic acid which is the same as an n-alkane substrate chain. According to the method, all sorts of single and mixed binary acids of C10-C18 can be produced, wherein in a 10-liter fermentation tank, when DC11 is produced from nC11 through fermentation transformation, the DC11 output in 144 hours reaches 165g / L, and the after-treatment yield is 88%; and when the purity of the nC11 is more than 99%, the purity of the DC11 reaches 99%.

Description

technical field [0001] The invention relates to a Candida lipolytica strain and a method for preparing long-chain dibasic acids, especially a method for preparing undecane dibasic acids, belonging to the field of microbial fermentation. Background technique [0002] Long-chain dibasic acids are important raw materials for chemical synthesis of high-grade spices, high-performance nylon engineering plastics, hot-melt adhesives, coatings, lubricating oils, high-temperature dielectrics, medicines and pesticides. DC 11 (Undecane dibasic acid) is an important raw material for the synthesis of high-performance nylon 1112, nylon 1110, nylon 1111, advanced fully synthetic lubricants and green pesticides. [0003] DC 11 It does not exist alone in nature and cannot be synthesized by chemical methods. Therefore, the special oxidase activity of microorganisms is used to ferment and convert n-undecane (nC 11 ) produces DC 11 , opens up the DC 11 The new source of Nylon 1111 and Nylon...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/44C12R1/73
Inventor 赵友谊
Owner 山东华星环保集团有限公司
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