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Compound microbial inoculum for deodorization

A technology of deodorant and agricultural microorganisms, applied in the direction of microorganisms, microorganism-based methods, deodorization, etc., can solve the problems of difficult large-scale application, high cost, sacrifice, etc., and achieve less environmental impact, good deodorization performance, deodorization, etc. Obvious effect of smelly effect

Active Publication Date: 2011-06-15
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because physical deodorization and chemical deodorization have the disadvantages of high cost, sacrificing part of fertility or difficulty in large-scale application, biological deodorization has become an important method to control stench

Method used

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  • Compound microbial inoculum for deodorization
  • Compound microbial inoculum for deodorization
  • Compound microbial inoculum for deodorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Preparation of culture medium and strains

[0045] 1. Preparation of culture medium

[0046] Acetobacter liquid culture medium (pH 6.8): composed of 100 g of glucose, 10 g of yeast extract, 20 g of calcium carbonate and 1000 ml of distilled water (15-20 g of agar is added to the solid medium).

[0047] GPYA liquid medium: It consists of 40 g of glucose, 5 g of peptone, 5 g of yeast extract and 1000 ml of distilled water (15-20 g of agar is added to the solid medium).

[0048] MRS modified liquid medium (pH 6.1-6.3): made of beef peptone 10g, yeast extract 5g, sodium acetate 5g, magnesium sulfate 0.1g, dipotassium hydrogen phosphate 2g, MnSO 4 .H 2 O 0.05g, sucrose 20g and distilled water 1000ml composition (solid medium added agar 15-20g).

[0049] Potato liquid culture medium (PDA): It is composed of 200 g of potatoes (peeled potatoes are cut into small pieces, boiled for 30 minutes and filtered to remove the filtrate), 20 g of glucose and 1000 ml of dist...

Embodiment 2

[0054] Example 2. Mutual antagonism experiment between strains

[0055] Activated Trichoderma viride (ACCC30166), Acetobacter pasteuri (ACCC10600), Candida utilis (ACCC20060), Saccharomyces cerevisiae (ACCC20065), Lactobacillus plantarum (ACCC11016) and Lactobacillus japonicus J were used for mutual antagonism experiments between strains , two or two combinations, there are 30 combinations in total, and each combination is three parallel.

[0056] The results are shown in Table 1.

[0057] Table 1 Antagonistic effect between bacterial species

[0058]

[0059]

[0060] Note: "-" indicates negative antagonistic response, "+" indicates positive antagonistic response

[0061] The test results showed that lactic acid bacteria J had inhibitory effect on Acetobacter pasteurii and Lactobacillus plantarum, and Acetobacter pasteurii had inhibitory effect on lactic acid bacteria J.

Embodiment 3

[0062] Embodiment 3. Determination of fermentation deodorization ability of single strain

[0063] The activated Trichoderma viride (ACCC30166), Acetobacter pasteuri (ACCC10600), Candida utilis (ACCC20060), Saccharomyces cerevisiae (ACCC20065), Lactobacillus plantarum (ACCC11016) and Lactobacillus J were cultured in liquid medium for 3 (Lactobacillus plantarum and Lactobacillus J were cultured at 37°C, and other strains were cultured at 28°C), and the bacterial liquid of each strain was obtained.

[0064] The bacterial liquid of each strain is respectively carried out the following experiments: the bacterial liquid is connected in the fermentation deodorization matrix, and mixed in a 500mL beaker (each part of the fermentation deodorization matrix is ​​inoculated with 10mL bacterial liquid), as an experimental sample, the fermentation deodorization matrix is ​​set. As a control sample, each sample was sealed with kraft paper and a layer of plastic paper. Three parallel treatm...

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Abstract

The invention discloses a compound microbial inoculum for deodorization, which comprises the following active ingredients: Trichoderma viride, Candida utilis, Saccharomyces cerevisiae and Lactobacillus plantarum. As a green technology, the microbial deodorization technology has the advantages of low environmental impact and ecological sustainability, and can not produce secondary pollution. The microbial inoculum disclosed by the invention has the advantages of favorable deodorization performance, obvious deodorization effect and high practical value.

Description

technical field [0001] The invention relates to a composite microbial inoculum for deodorization. Background technique [0002] In recent years, environmental problems have attracted more and more attention, especially the environmental problems caused by solid waste have become increasingly prominent. Solid waste includes livestock and poultry manure, domestic waste, etc. No matter in developed or developing countries, livestock and poultry manure has become the main source of solid waste, causing serious pollution to the atmosphere, water and soil, and endangering human life and health. In 2000, the annual output of livestock and poultry manure in my country reached 1.73 billion tons, which was 2.7 times that of industrial waste. Due to the relatively weak solid waste deodorization treatment system in my country, a large amount of solid waste emits unpleasant odors and breeds mosquitoes and flies, which seriously damages people's living environment and even poses a threat...

Claims

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Application Information

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IPC IPC(8): C12R1/885C12R1/25C12R1/865C12N1/14A61L9/01C12N1/20C12N1/16C12R1/72
Inventor 顾金刚李世贵龚明波姜瑞波张晓霞阮志勇马晓彤张瑞颖
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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