30 results about "3-Hydroxyhexanoate" patented technology

Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.

The present invention relates to a film comprising a biodegradable copolymer, wherein the copolymer comprises at least two randomly repeating monomer units (RRMU) wherein the first RRMU monomer unit has the structure and the second RRMU has the structure wherein at least 50% of the RRMUs have the structure of the first RRMU. The present invention further relates to an absorbent article comprising a liquid pervious topsheet, a biodegradable liquid impervious backsheet comprising the above film, and an absorbent core positioned between the topsheet and the backsheet.

An elastomer composition is provided which contains a matrix of poly-(3-hydroxybutyrate-co-3-hydroxyhexanoate) produced from microorganisms and crosslinked natural rubber particles dispersed like islands in the matrix. An eraser using the elastomer composition is also provided. The present invention is capable of providing an elastomer composition and eraser using the same, which can be recycled, are friendly to the environment, and corresponds to the recycling society, without relying on fossil resources.

Several novel PHA polymer compositions produced using biological systems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3-hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition. Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, 63: 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.

The invention discloses an absorbable suture containing medium-chain PHA (Polyhydroxyalkanoate) (PHBV (Polyhydroxylbutyrate Valerate) or PHBHHx (poly (3-hydroxybutyrate-co-3-hydroxyhexanoate))). The invention specifically discloses a PHBHHx monofilament fiber, a PHBHHx / PLA (Poly Lactic Acid) monofilament fiber and a PHBV / PLA bunched fiber and preparation methods thereof. Blending with the PLA is performed, so that a spinning process is simple, complementary material performance is achieved, and the PHBHHx and the PHBV fibers are good in handfeel. Compared with the conventional material, the absorbable suture has the advantage of higher biocompatibility in the field of nerve and vascular repair due to the use of the PHA material. Moreover, the PHA is high in mechanical strength and slow in degradation speed, so that the PHA is excellent in application effect on the suture repair of cartilages and bone tissues. On the aspect of novel minimally invasive surgical repair, the PHBHHx and the PHBV contain nontoxic and non-irritant degradation products with high compatibility, so that surgical scars can be effectively reduced, and the absorbable suture has an application prospect in esthetic restoration.

The invention relates to the production of polyhydroxyalkanoate copolymer with high 3-hydroxyhexanoate monomer content through recombinant gene expression.

The present invention intends to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material. In accordance with the present invention, there is provided a method of producing a microorganism that produces poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material, by introducing a gene encoding R-hydratase that converts a fatty acid β-oxidation system intermediate to a monomer, (R)-3-hydroxyacyl-CoA [R-3HA-CoA], into a recombinant Cupriavidus necator strain that was conferred an ability of producing P(3HB-co-3HHx).

An objective of the present invention is to provide a biodegradable polyester fiber which has excellent thermal stability and excellent fiber strength. Another objective of the present invention is to provide a method for producing a biodegradable polyester fiber which has excellent mechanical properties, especially excellent thermal stability. The present invention relates to a biodegradable polyester fiber which is composed of a poly(3-hydroxybutylate-co-3-hydroxyhexanoate) that has a 3HH molar fraction of 2-9% by mole. The present invention also relates to a method for producing the biodegradable polyester fiber, which is characterized by comprising a fiber formation step wherein a poly(3-hydroxybutylate-co-3-hydroxyhexanoate) is formed into fibers at a temperature that is not less than the glass transition temperature of the poly(3-hydroxybutylate-co-3-hydroxyhexanoate) but not more than 70 DEG C during the melt extrusion of the poly(3-hydroxybutylate-co-3-hydroxyhexanoate).

The invention discloses an absorbable suture containing medium-chain PHA (Polyhydroxyalkanoate) (PHBV (Polyhydroxylbutyrate Valerate) or PHBHHx (poly (3-hydroxybutyrate-co-3-hydroxyhexanoate))). The invention specifically discloses a PHBHHx monofilament fiber, a PHBHHx / PLA (Poly Lactic Acid) monofilament fiber and a PHBV / PLA bunched fiber and preparation methods thereof. Blending with the PLA is performed, so that a spinning process is simple, complementary material performance is achieved, and the PHBHHx and the PHBV fibers are good in handfeel. Compared with the conventional material, the absorbable suture has the advantage of higher biocompatibility in the field of nerve and vascular repair due to the use of the PHA material. Moreover, the PHA is high in mechanical strength and slow in degradation speed, so that the PHA is excellent in application effect on the suture repair of cartilages and bone tissues. On the aspect of novel minimally invasive surgical repair, the PHBHHx and the PHBV contain nontoxic and non-irritant degradation products with high compatibility, so that surgical scars can be effectively reduced, and the absorbable suture has an application prospect in esthetic restoration.

An object of the present invention is to improve spinnability, productivity, and tensile strength of a polyester fiber containing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).By spinning the polyester resin containing the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) at a high spinning velocity, the spinnability, productivity, and tensile strength of the polyester fiber can be improved.

Polyhydroxyalkanoate (PHA) synthase gene (SEQ ID NO:2) isolated from fresh water Chromobacterium sp. USM2, its amino acid sequence (SEQ ID NO: 1) and its use to produce polymers incorporating C3 to C7 monomer units, including poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) random copolymer P(3HB-co-3HHx), poly(3- hydroxybutyrate-co-3-hydroxycalerate) random copolymer P(3HB-co-3HV) and P(3HB-co-3HV-co-3HHx) terpolymer. Production of polymer by expression of SEQ ID NO:2 in recombinant hosts including microorganisms such as Cupriavidus or Pseudomonas.

An object of the present invention is to improve spinnability, productivity, and tensile strength of a polyester fiber containing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).By spinning the polyester resin containing the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) at a high spinning velocity, the spinnability, productivity, and tensile strength of the polyester fiber can be improved.

The invention provides a poly(3-hydroxybutyrate-3-hydroxyhexanoate)-carboxymethyl cellulose-straw powder composite full biodegradable material. The material is prepared by the following steps: mixingpoly(3-hydroxybutyrate-3-hydroxyhexanoate) (PHBHHx) and straw powder, adding a certain amount of carboxymethyl cellulose, phosphate coupling agent and polyoxyethylene, putting all the materials into athree-dimensional mixing machine for mixing, transferring the mixture into a mould, and putting the mould onto a plate vulcanizing machine for compression molding, so as to obtain a composite material. Compared with a poly(3-hydroxybutyrate-3-hydroxyhexanoate) product, a product prepared from the poly(3-hydroxybutyrate-3-hydroxyhexanoate)-plant fiber composite material has great advantages that the hydrophilicity of the material is improved, the mixing efficiency of the composite material is increased, and the processing property of the composite material can be obviously improved; the mechanical property and the mechanical property of the prepared product are greatly improved; the prepared composite material is high in tensile strength and strong in impact toughness, therefore, the composite material is a full degradable material with good environmental protection value and economic value.