The invention relates to the battery field and in particular relates to a battery for analyzing in-situ spectrum and a using method and application of the battery. The battery comprises a top cover, a sealed sleeve pipe, a shell, a shell liner pipe, an electric conductive compression column, an electric conductive spring, an electric conductive pressure sheet, a working electrode post and a reference electrode post; the assembling steps are as follows: inserting the shell liner pipe into the bottom of the shell, sequentially placing a working electrode, a battery diaphragm and a reference electrode into the shell liner pipe sequentially, enabling the working electrode to be positioned at the bottom of the shell and at the opposite side of a watch window; placing the electric conductive pressure sheet, the electric conductive spring and the electric conductive compression column into the shell liner pipe sequentially, and tightly pressing the electric conductive pressure sheet on the reference electrode; connecting the outer side of the shell and the inner side of the sealed sleeve pipe and connecting the outer side of the sealed sleeve pipe and the inner side of the top cover by threads; screwing all threaded connecting parts to realize sealing of the battery; and connecting the working electrode post and the reference electrode post with the shell and the top cover respectively. The battery can carry out in-situ spectrum analysis research on the surface of the electrode in charging and discharging processes.