The invention belongs to the technical field of
plant microscopic observation and discloses a paraffin sectioning method for
eucalyptus tissue. The method comprises the steps of preparing materials, fixing, washing, dehydrating, performing a transparency treatment,
waxing, embedding, sectioning, displaying the sections, adhering the sections,
roasting the sections, dewaxing and rehydrating,
dyeing, dehydrating, redyeing and enclosing the sections. According to the paraffin sectioning method for the
eucalyptus tissue, utilized novel stationary liquid polyhydroxyl
acrylic acid has high speed, can largely shorten the fixing time and can balance the fixing; meanwhile,
xylene replaces with a safe and nontoxic TO transparent
reagent, the TO transparent agent can be solved with
alcohol of 95% at 25 DEG C or above, can solve paraffin and sealed vegetable glue, has excellent volatility without bad influence on
dyeing of common biological dye, and has relatively better transparency and dewaxing effects, and after the transparency treatment is performed, the tissue is unlikely to embrittle, the sections can keep complete; furthermore, neutral vegetable glue replaces with a section sealing agent CVmount. After the fixing, dehydrating and section sealing steps during optimization of paraffin sections are performed, the section
production cycle is shortened by at least 72 hours on the premise of ensuring the sectioning effect.