Paraffin sectioning method for eucalyptus tissue

Abstract

The invention belongs to the technical field of plant microscopic observation and discloses a paraffin sectioning method for eucalyptus tissue. The method comprises the steps of preparing materials, fixing, washing, dehydrating, performing a transparency treatment, waxing, embedding, sectioning, displaying the sections, adhering the sections, roasting the sections, dewaxing and rehydrating, dyeing, dehydrating, redyeing and enclosing the sections. According to the paraffin sectioning method for the eucalyptus tissue, utilized novel stationary liquid polyhydroxyl acrylic acid has high speed, can largely shorten the fixing time and can balance the fixing; meanwhile, xylene replaces with a safe and nontoxic TO transparent reagent, the TO transparent agent can be solved with alcohol of 95% at 25 DEG C or above, can solve paraffin and sealed vegetable glue, has excellent volatility without bad influence on dyeing of common biological dye, and has relatively better transparency and dewaxing effects, and after the transparency treatment is performed, the tissue is unlikely to embrittle, the sections can keep complete; furthermore, neutral vegetable glue replaces with a section sealing agent CVmount. After the fixing, dehydrating and section sealing steps during optimization of paraffin sections are performed, the section production cycle is shortened by at least 72 hours on the premise of ensuring the sectioning effect.

Description

technical field [0001] The invention belongs to the technical field of plant microscopic observation, in particular to a method for paraffin sectioning Eucalyptus (Eucalyptus) tissue. Background technique [0002] The application of paraffin section technology has a history of more than 300 years, and it is one of the most widely used methods in the routine preparation technology of plant histology. Paraffin section can not only be used to observe the morphological structure of normal plant cells and tissues, but also the main method for observing, researching and judging the morphological changes of cells and tissues in pathology and forensic science, and it has also been widely used in basic biology and other fields. research in the subject area. In recent years, with the development of biological science and technology, and the rise of plant ecological anatomy and molecular biology, paraffin section technology has been more actively used in the field of plant science res...

AI Technical Summary

Problems solved by technology

However, xylene is a highly volatile and highly toxic chemical reagent, which has a strong toxic effect on human breathing, hematopoiesis and nervous system, and is also one of the carcinogens, seriously endangering the health of those who have been engaged in tissue section experiments for a long time; in addition, two When toluene is used for slicing, it has a strong ability to penetrate tissue, and it is difficult to grasp the clearing time. It is easy to make the sliced ​​tissue shrink, harden and become brittle, which increases the difficulty of slicing. It is easy to form waste pieces and waste materials.

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